XB-CLONE-2421408
Clone Name: prmt1-a-NP241
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Gene:
prmt1.S
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Species: Xenopus laevis
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Description: EXRC Clone Number NP-241.
Xenopus laevis protein arginine methyltransferase 1.
To generate probe for in situ.
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Sequence:
5' EST: AI031386 [+]
agtgaatggatgggatattgtctattctatgaatcaatgcttaacactgttatctatgcacgtgataaatggctgactccagatggattaatatttccag
atcgtgctacactgtatataacagctattgaggatagacaatacaaagactacaagattcactggtgggaaaatgtttatggctttgatatgtcttgcat
taaggatgtagctatcaaggagcctctggttgatgttgttgaccccaagcaactggtctcaaacgcttgccttataaaggaagttgatatttatactgtc
aaagtggatgacttgagttttacctctccattctgcctgcaagtgaaacgaaatgactacatccatgccctggttgcatatt
3' EST: AI031387 [+]cactgttcactttttaatttcattatgaatgcaaccatataaaaaccataagttatgaaacagtcacaatgggaagctttgaaggatccatgaaacaaat
aaagggcctttccataccaagatctgagaaacttacaaaaacagtagaaaacaccaagccaagtgagtaacccatttgttcctcaataggatggatgtct
gctccacagaagggatagacggcaatattggtgaaaccagcaactgccaacaagacaattcccaaccaaataagaaaaaaaatcctgcaaaaatagtgaa
aaaacgggaatgcacaatttggagcttaattttgctgcc
Library:
Description: Xenopus laevis polyA+ RNA embryonal stage 13 were used as source material, and directionally cloned into pBSKS+. For antisense production, plasmid should be cut using SalI, and RNA should be synthesized using T7 RNA polymerase.Vector Details | Vector Map |
Name: pBluescript KS+
Type: phagemid
5' Restriction Site: SalI
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
SalI |
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Suppliers:
Search for clone at:
EXRC