XB-CLONE-2354796
Clone Name: IMAGE:3745143
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Gene:
XB5772073.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC097845 [+]
CGGTCGGAGGGGAATCTCTCCACCCTCTGCCCTTCTGGAAACAACTCGGATAATTCAGCCCATTCCATCTCTCCAGCCGTGAGGACTTAATCAGAAGACC
AACCGCAAGGATTTAATGTAGCGAATGACTCCCTAAAGGCTGCACAGGCATTTCAGGCTGATTGAAGGGACAAGTATTCGGCTTAAAGTCCAAGAGATCT
TTCCCTATGACTGAGAAGGACAAGGATGTCTGGCAGGAGGACCCCGCGGAATACTGTCCAGGTGGTTACCACCCAGTGCAATCTGGAGATATGCTGAACA
GAAGATACCAAGCCATTCACAAGGTGGGATGGGGCTACTTCTCTACTGTTTGGCTGTGTCACGATTTACAAAAAAAGAAGAAGGTGGCAGTGAAAATTTC
CAAAAGTGGACGAAGGTTCAGCGAAGCGGCCCTGGATGAAATCTCTATTCTTAATTGTGTAAATGGTGCACGGAAGAAAGAGTCTCAAGGGGAGAATGTC
ATCCAGCTTTTAGATGACTTTAAATTGATCGGAGAAAACGGCCTCCATGTGTGTCTGGTATTTGAATTGCTGGGACCATCTCTCCTGCATTTAATGAGGA
ATCACGGCCCGGAAGGCCTGCCTCTAACGTGTGTGCGTAGGGTTCTCCAGCAGGTGCTGCAAGGCCTTAACTTTCTTCACAAGAGGTGCCGCATTATCCA
TACAGACATCAAACCAGAAAACATCCTTGTGTGTGTGAAGGCGGACAATCTGCAGCAGTGCATGGCTGAAGCTGCTATCTGGAGCCAGAATAAAGCTGGT
GACCGTACGGAGCATGGTGTTGATGTGAACTTCCTAACTCACCTGTTTGAGACTGGAAATTCCGACATGCTGGGAGTGAAAATCGCAGACCTGGGCAGCA
GTTGCTGGACTTATAAAGCCTTTTCTGAGGAAATCCAAACCCAACAGTACAGGGCACCGGAGGTTCTTTTGGGCTCAACCTATAGCACTTCTGTGGATAT
ATGGAGCACAGCCTGCATGGCATTTGAAATGGCCACGTCATACTATCTGTTTGAGCCCCACGCTGGAAAAACCTTCACCAGAGAGGATGATCACATTGCC
TGTATAATGGAACTGCTGGGGCGCATCCCACCCAAAGTTATTTCTTCTGGGAGAAAATCACCTGCGTTCTTCAATAAACAAGGGGATCTGCTCCGTATCC
CTCAGCTGTACCCCTGTGGGCTTTACGACACCCTCGTGAGAGGGCACAGATGGCAGAAGAATGAGGCCCTTACATTTGCCAGCTTTCTCCTGCCCATGCT
GGAATATGTCTGTGAAAAACGGGCAACCGCTGAAACGTGTCTTCAACACCCGTGGCTCCAGTGCTGAGAGCGAGAGAGACTGTATTTCTCTGCACATCAT
AGGTTATTTTTTTATATGAATCACTCCTTACCTTGGAAGTGGATCTCTCTGCCAGAATGTCATTTGCTTTAATGCACCACGTGAGTGCCAGGATGGTGCC
CTCCCAGCATGCTTAGCAGCCCCGCTATCAGTGATTAGGTGAAATGCAGAAAGGACATCAGAATTGTAGAAGACAAGGCATATAATGAATTTCCCCAGGG
TGCATTGCTGCTAATCATCAAATCATTGTACCTTGCAGGTCACATGGGAATTAGAATTTGTAATATAGGGGTTTTCTCCTGTATATTCTCCACTTGGTTA
AACTTGATGAAACTTTTTCAGCCTTGTCCACTATGTTGTTGTAAATACATTCTTGTAACCAACAGAACCCTATTTTCCATGAAATAACAATAAAGCATCA
TCTCACCGCTTAAAAAAAAAAAAAAAAAA
5' EST: BE680155 [+]ggaatctctccaccctctgcccttctggaaacaactcggataattcagcccattccatctctccagccgtgaggacttaatcagaagaccaaccgcaagg
atttaatgtagcgaatgactccctaaaggctgcacaggcatttcaggctgattgaagggacaagtattcggcttaaagtccaagagatctttccctatga
ctgagaaggacaaggatgtctggcaggaggaccccgcggaatactgtccaggtggttaccacccagtgcaatctggagatatgctgaacagaagatacca
agccattcacaaggtgggatggggctacttctctactgtttggctgtgtcacgatttacaaaaaaagaagaaggtggcagtgaaaatttccaaaagtgga
cgaaggttcagcgaagcggccctggatgaaatctctattcttaattgtgta
3' EST: BE678729 [+]tttttaagcggtgagatgatgctttattgttatttcatggaaaatagggttctgttggttacaagaatgtatttacaacaacatagtggacaaggctgaa
aaagtttcatcaagtttaaccaagtggagaatatacaggagaaaacccctatattacaaattctaattcccatgtgacctgcaaggtacaatgatttgat
gattagcagcaatgcaccctggggaaattcattatatgccttgtcttctacaattctgatgtcctttctgcatttcacctaatcactgatagcggggctg
ctaagcatgctgggagggcaccatcctggcactcacgtggtgcattaaagcaaatgacattctggcagagagatccacttccaaggtaaggagtgattca
tataaaaaaataacctatgatgtgcagagaaatacagtctctctcgctctcagcactggagccacgggtgttgaagacacgtttcagcggttgcccgttt
ttcacagacatattccagcatgggcaggagaaagctggcaaa
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: