XB-CLONE-2354732
Clone Name: IMAGE:3745226
|
Gene:
sinhcaf.L
|
Species: Xenopus laevis
|
|
|
Sequence:
Full length sequence: BC100243 [+]
ccgcggatcagtttgcagatcagtcagttgcgggtcaggtccggactttccctcgctgagagccgataggcccccgtgacaattgcaccttcacagcaat
gaccttgggtgccgctggccaggctttgggtgtacagaacgttcaagcaagagatggggtatgtaggacagaaaatagacatccaccaaaagtcttaacc
tacttgaccttcaagatgatctttcaggaaaaaagacatccaacatcttatcttaactggccttcaaacttggcattcaggggaacccaggagtagggcc
gccatcagggggggacaggaggtacgagtggcccgggcctgaaggggggggggggcggcagtgcttccaaaaacatttgctagaattcctaaacccgcct
ccctgtgcccgctggagatcggccatctacggaactgatcggaatgtctcggcttcttccgcgacgtgggggaccgagtaaataaacgagagagagctgg
agagcccactaatgatgacatcacttcctgctttatggtggtcagcgtgtcgcggatcagtttgcagatcaggcagttgcgggtcagaaaacaccacgag
gaaaaaacgccaacaactggaagtaccgagaggctccggtgaaggatccaaagaaaacccgacatctatcagcgcaactcccgtgtgctaaaaataaaag
attgtctcccacaaaccggcattacagcaatggattccccttggcaacaggcagcgacatgtacaaccggcaacaagagacgatcgctactaccaatcag
gactgtgcgtataattcattaagggccgactgcatgtgagagtcgctaagtgtaattacccgcagacgattcttcaattcctgacggtacttcactctcc
tcagtttaaagcaacaggtacagtgagcgctggggacagacggaagacgagccctgctcccatataaaaggcaaacacatgggcatctaagtcccacttc
tgatgacgtcacttcctgtttccagcaacggcacttcctgctttatggtggtcagcttgtcgcggatcagtttgcagatcaggcagttgcgggtcagtga
ggcgtgggacgccaagagaatccagcgcccagagacagatccatgggccgcctgtattgcaaagaaggctagcggtccggacttcccccgctgagagccg
ataggcccccgtgacaattgcaccttcacagcaatgaccttgggtgccgctggccaggctttgggtgtacagaacgttcaagcaagagatggggtatgta
ggacagaaaatagacatccaccaaaaggcttcacctacttggccttcaagatgatctttcagtgatatttacgaaaaaagacacccaacatcttatccta
actgggtgtcaagctctacagtctgggaacctcagataagaacccaacacaactttgccacacacacaccatcggaaaccacagcgcgtctctgagcaac
gtttgcacggatagaagactaaaagctcattagaatctcccggcagaataattatttccatgttattgcgtgtgattattgagtgggagtccatagagca
gacctacaaggattcacttctatcagcaaagacacgttttctcctccacaataaatggcccacttctgatgacgtcacttcctgtttccagcaacggcac
ttcctgctttatggtggtcagcttttcgtggatcagtttgcagatcaggcagttgcgggtcagcccacttctgatgacgtcacttcccatttccagcaac
agtacttcctgcttcatggtggtcagcgtgtggcggatcagtttgcagatcaggcagttgcgggtcagagccttccctatctgttccttctcctctggac
taccggccagctgaagattccagggcaactgttctattcactgctgcctcagcaacgctggaattcccacattgtgttccaagcctcactttggcgctgg
ttggttgatatcagtaagccccgcccatcgccgaggagcaggagctgcacgtggagcaggtgagggggaggtggctgtaaagttgcagatggaaatattg
gggacaagagaagatattggaggacacagaagggattttcgggtgcaggagaagattctttttcggggtctcagccagagaagaacaagtgacattggta
taaaacatcccaacactcttcgttctcagtttggagcgggacctcttggaactgcgttatagcttggtgagagacgcgtctatggacaggtgacaagagc
tggtcttgccgaagctgtacgtctgacaggcctgttcgttgacggtgtcgaaatcgtatcctttcttgggcggatgctcgctgtgcggggaggacaccat
gaagtggccgctgtggatgatctgcgcctcggagctgtccgactttttaagcagtaattcaggatcgtccgttgtcagtcatcgtccggctcttgcttga
ccatgatgctggggtgtgacagacgcaccggtagcatttcagccatcatcacccctcccgttcccagccccagaaaggcgaatgaggccgggggagagtt
agacgtcacgcaaaagcccaaccaatggagaagttggaggcgctgtcactgggcgtggcttagcgtgattggtttacaggctgaccggccttcgacgacg
cccactcgatgacgttgccagtaggggtgtctgggttgttggtgaaggcgcgttgggatgctggagctgggcggaacgaatgaaagaaaattgctccctg
ccaatgtataaccttgatgacgctcctgacccagttgccaaggatcaagtagtccgagagtcagatttgttattaaactccatggatcacggtgacgcca
aactcaatgggcagttggaagcctcgttccccggctgcttccgtcatggctaaagttactgatcaactcgcagttcaagagctcgagtggtcgatgcccg
aggagggcccaacctgaaagcaatgaggatacgtcccaaaaagatccgaccctttgcctcgcgaaggcatcaaagcaagcaagtggcggagctgaagagc
ggtatctcaagtctatactgtggagcatcgagcacctccccagagcattcctcgtcctgcagcaatcactcccatactgacaccgacactgaactctgca
tccagcaccgactccggcttctctttcctacaccccgcctactggaaaagtgactacagtttgacatttcagcagctatctggttgctagggtactgctt
atccttaacaaccgcccatcggttggagtggctggaaggtgaatagggacaatgaccatttagcctcacaaagctgctggggtcggtgaccccgttggga
ctctgataagaagcagaagaccagttgaaaggttgctaagaagaggactttctataacatacttatgttaatgtaaagcagcactggccctttaaagaag
tggatcccatggggcagactgtaatatgtgtaatattaaatgcatggggtttactgtacacaccacgttgtgcctcaatatgcagagggtgcctctaaat
aaatgggggggagcctttgttagcatttattaagctattatttatacttcccctttatcctgtgaacccaaatcctcctcattagggttggactcagtgt
gtatatacagtatattcaggcagctattaatacttctggatactggaagtatgggagatttctttggggtgtatatgagaccccccctgaatcttttaac
cgtctaatctccgtgtgagatttgtgacgctcgtgtttgtacatggatgttttataatgtagattttgtacctctgtatagggattattattctttctac
aataaactttatatacttgtaaaaaaaaaaaaaaaaaaa
5' EST: BE680187 [+]atcagtttgcagatcagtcagttgcgggtcaggtccggactttccctcgctgagagccgataggcccccgtgacaattgcaccttcacagcaatgacctt
gggtgccgctggccaggctttgggtgtacagaacgttcaagcaagagatggggtatgtaggacagaaaatagacatccaccaaaagtcttaacctacttg
accttcaagatgatctttcaggaaaaaagacatccaacatcttatcttaactggccttcaaacttggcattcaggggaacccaggagtagggccgccatc
agggggggacaggaggtacgagtggcccgggcctgaaggggggggggggcggcantgcttncaaaaacatttgctagaattcctaaacccgcctccctgt
gcccgctggagatcggccatctacggaactgatcggaatgtctcggcttcttccgcgacgt
3' EST: BE678749 [+]aatgaggaggatttgggttcacaggataaaggggaagtataaataatagcttaataaatgctaacaaaggctcccccccatttatttagaggcaccctct
gcatattgaggcacaacgtggtgtgtacagtaaaccccatgcatttaatattacacatattacagtctgccccatgggatccacttctttaaagggccag
tgctgctttacattaacataagtatgttatagaaagtcctcttcttag
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: