XB-CLONE-2354640
Clone Name: IMAGE:3745285
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Gene:
arhgap5.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC133756 [+]
GCTTCCGAGGCGGTCGCCATTACGGATTAGTGGGAAAAAAGCTGTGAGGAAGCGAAGAGGTACCGGAGCCGGGGAAAGAGAGAAAAAGCCGGAGCCCAGC
CGGTGACGAGAAAGGAGAAAGAAAGCGGAGAGATATTAGGGAAGAAAGTGCGTGATGAGGCCAAGGAAGGCGATGGATTCGTGAGACAGAAAAAAAAAAC
CAAGCAATTAAATCCACCAACACGAAGGAACTGGGAGAGCAACTACTTTGGAAAGCCGCTGCATGAGCTTGTGAGCCCTGAGAAACCTATTCCAGTCTTT
GTGAAGAAGTGCGTGGAATACATTGAGGAGACAGGGCTGAGTGCAGAAGGTCTGTACAGAGTCAGTGGTTACAAGACTGATCAAGATAACATACAGAAAC
AGTTTGATCAAGATAACAATCTTAATCTGGCTTCGATGGAGGTGACTGTGAATGCAGTGGCGGGTGCACTAAAGGCGTTTTTTGCAGACTTGCCCGCGCC
TCTCATACCCTATAACCTTCACCTCGATCTGCTTGATGCCTCAAAAGTCCCAGAGAAAATGGAACGGCTGAAAGTGCTAAAGGATATTCTGCGGAGCTTT
CCTCCTGTCAACTATGATGTCCTGAAATTTATCATTGCTCACTTGAACAGAGTCAGCCAGCACAGCAAGACCAATCTGATGACGGCCGATAACCTTTCCA
TCTGCTTTTGGCCCACTCTGATGCGGCCAGACTTTGAAAGCAAGGAGTTTTTCTCCACCACCAAGAACCACCAATCCGTCATTGAGACGTTCATCGTGCA
GTGCCAGTTCTTTTTTTATGAAGGTGACATTGTGGATTCGCCTAGTGCCACCAGCTCTCCGTCAGCCCAGCACTCCAGCCCGGGGCCATTAGCCGCACCC
CTTGTTCCTCTACAGTTACCACCCCCTCTCCAGCCGCAGCTGCTGCAGCCCAACTTGCAGGCAGATGCAATGGGCATTCTATAAATTGCGAAAAAGCTGA
GTGCACTGTTATTTCTTAACCACTGTCTTTGCAAGTTCGATTTGCCACGTAGCAATGGCTTCCCGACGCTCCTGTAGTAATATCTTAATGACGCAGAAGA
TTCAGATTATTTTTAGTTTTTTTTTCTTTTGTATTTTGTAAAAAAAAAAAAAAAATACACCAGGACTTGTAGAGAGAAAGCTGTGGCAAGCCTTGTTTGT
GAAATTTGCTGCTGCATGGAACTTATATAGAAATATTATACTCTTCACATATGGCACTGTCATGCCGTGGATATACTTATCAGTACAGAGGCTTAAACTA
CAGAAGAAAAAGAAAACCGTTCTTTTCTATAGAAAAATCTAATTAAAATAAATTACATTTATGAAGCTGTTAGAGTGCTTTTCCCAGCCCTGCTGCAGGC
TGAGATTTATTTTCACATCAAAGCATCTGTGGTGTGTGTACGTGGGCATGTGTGGGTGTGTGTGGGAGCCTGGCAGTGGGGTGGGCAATTGGAATGGAGC
TTAACATGTAAATGAAACAAAATAGTAATTGTATGTACATATATAAATGTTTACATATTTCAGGAATAAAATCCATCCACTGAAAAAAAAAAAAAAA
5' EST: BE680233 [+]cggtcgccattacggattagtgggaaaaaagctgtgaggaagcgaagaggtaccggagccggggaaagagagaaaaagccggagcccagccggtgacgag
aaaggagaaagaaagcggagagatattagggaagaaagtgcgtgatgaggccaaggaaggcgatggattcgtgagacagaaaaaaaaaaccaagcaatta
aatccaccaacacgaaggaactgggagagcaactactttggaaagccgctgcatgagcttgtgagccctgagaaacctattccagtctttgtgaagaagt
gcgtggaatacattgaggagacagggctgagtgcagaaggtctgtacagagtcagtggttacaagactgatcaagataacatacagaaacagtttgatca
agataacaatcttaatctggcttcgatggaggtgactgtgaatgcagtggcgggtgcactanaggcgtnttttgcagacttgcccgcgcctctcataccc
tataaccttcacctcgatctgcttgatgcctcataagtcccagagaaaatggaacggctgaaagtgcctaaagggaatattctgcgagctttc
3' EST: BE678795 [+]ttcagtggatggattttattcctgaaatatgtaaacatttatatatgtacatacaattactattttgtttcatttacatgttaagctccattccaattgc
ccaccccactgccaggctcccacacacacccacacatgcccacgtacacacaccacagatgctttgatgtgaaaataaatctcagcctgcagcagggctg
ggaaaagcactctaacagcttcataaatgtaatttattttaattagatttttctatagaaaagaacggttttctttttcttctgtagtttaagcctctgt
actgataagtatatccacggcatgacagtgccatatgtgaagagtataatatttctatataagttccatgcagcagcaaatttcacaaacaaggcttgcc
acagctttctctctacaagtcctggtgtattttttttttttttttacaaaatacaaaagaaaaaaaaactaaaaataatctgaatcttctgcgtcattaa
gatattactacaggagcgtcgggaagccattgctacgtggcaaatcgaacttgca
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: