XB-CLONE-2343342
Clone Name: IMAGE:3744793
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Gene:
pus7.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC100242 [+]
CCTTGTCTACGCTGCTCTACCACGTGTTTGGCCGCTAGATGCTAAGGTTCCTGTCGCGAGTCGCATTTTGCAGTTACAGGCATCTTGTTTTTGGTTATTT
GAAACCCTTTCATCGTGCCTTAAAAGCAATGGAATCCATCGAAATGGATTCTGTTTCTCTAAAACGGCCTATTTCTAGCAAAGACCTTAGTGGTGATTCT
GTAAAAAGGCAAAAAGTCTCGGACAATTTGTTCTCAACAGAGACTTCTTTAAAAGCACAGGATGTGGAACCTACTTCAGGGATTCAGAATGAAAATGCCT
TAGATAGAGAACTTACACTTGAAGTGCCAGGAGAGAAAAATGAAGAAGATTTTGAGAAAGACGAGCTTCTGGATGGGGAAACTGAAGAAGAAAGTCCAGA
GAGCTTTGCAGACATGATGAAGCATGGCCTTACTGAAATTGATGTTGGCATTACTAAATTTGTAAGCACTCATGAAGGGTTTTCTGGAATTCTGAAAGAA
AGATACTCAGACTTTGTTGTCAATGAAATAGGGAAAGAAGGCAAGATCCTTCATTTAAATGATGTTTCAGTACCAGCAGATGAAGAGGATCCTTCTGAAG
ATATATATTCTGTTCTCTCACCTGATGATAAAAAGCGTTTGGAAGATCTTCAGCTGTTTAAGAATAGGGAAGATAATGTTGCGATTGAGGTTGTTGAAGA
CACTAAAGAAAAAAGAACACTTATCCACCAAGCTGTAAAGGGTCTGTTTCCTGGGATAGAGACTAAAACAGAGGAACGAGAAGGGAAGAAATACATCATA
GCTTACCATGCCGCTGGCAAGAAAGCATTGTCAAATCCTCGGAAGCATTCCTGGCCAAAAGCCAGAGGAAGCTACTGCCATTTTGTTTTGTACAAAGAAA
ACAAAGACACCATGGATGCCATAAACGTGCTCTCAAAATTTTTAAGAATCAAACCAAATATATTTTCCTATATGGGTACCAAAGATAAAAGGGCTATCAC
TGTTCAAGAAATTGCAGTTCTTAAAATTACAGCACAAAGGCTAGCTCATCTAAACAAGTGCTTGATGAACTTCAAATTAGGTAACTTCACTTACCAAAAA
AGCCCGCTAAAATTAGGTGAACTTCAAGGAAATCATTTTACTGTGGTGTTAAGAAATATTACTGGGACAAGTGAACAAGTAGTGCAAGCCATGACTTCTC
TCAGGGATATTGGCTTTATAAATTACTATGGAATGCAAAGATTTGGAACCACAGCTGTACCTACATACCAAGTTGGAAGGGCTATTCTTCAAAGTAACTG
GAATGAGATGGTAGATCTAATATTAAAACCAAGGCCTGGAGCAGAGAAAGGTTACCTGGTTCAGTGCAGAGAAGAGTGGGCTAAAATCAAGGACCCAGAG
GCTGCTCTTAAAAAGTTACCTGTTAAGAGATGTTTGGAAGGTCAGCTGCTGCGGGGACTTGCAATGTACGGTCTCAAAAACATAGTTTCTGCATTTGGCA
TAATCCCAAGAAACAACCGTTTAATGTACATCCACAGTTATCAGAGTTATGTTTGGAATAACATGGTAAGCAAGAGAATCGAGGAGTTTGGACTCAAAGT
TGTTCCCGGAGACCTAGTGCTCAAAGGAGACACTGCTGTTCAAGTGGAAGAGGGCAATGTTGATAATTACACAATAAACGACATAGTAATGCCGTTGCCA
GGCTTTGACGTGATCTACCCTAAGCACAAGATCGCTGCAGCTTACGAGGAAATTCTTGCAGCAGACAATCTTGATATCAAAAACATGAGGCACAGAGTTA
AGGATTATTCTTTATCTGGAGCCTATAGGAAAATAGTTATCTGTCCACAAGATGTTAGCTGGGAAGTTGTATCCTATGATGACCCTAAAATACCACTAGT
TCAAACAGATCTGGATAAATTGGAAGGGAAGGCGCTTCCAGAATATAACAAAGAAGGGAAATACAGAGCACTGAAGATGGAATTCTCATTGCCACCTTCT
ACATATGCGACAATGGCCATCAGAGAAGTTCTTAAAATGGACACCAGTATCAAAAACCAGACCCAGCTGAACACTGTCTGGCTTCGCTAAATTTTCTTAT
GAACTCACTGCTGTCCTTTCAGTGTCCCTTTTGGCACTTGTGATGACCATTTTATTGTTTAAAAAGATCTATTGGTTTCAAAAAGTCTTTTAATTTGAGA
GCTGTTTTTGTTACAGATTTGCAGTGCACAAAAATACATGATAAATTCAAAAATGACTGAAGAATATCTATAATACACTGGTCATTTTTACTGTTCTATA
AGTTGTATTTCTCCTTTTTTTCAAAGGTGTACACATAACTAGGTAGTAACTTAAGTTAAACCAGACTGAGTTGAAAGAACATGTAAAATTTTAACTTGAA
TAGGATAGTTCTATTTTTTTTTTTTTATAAATAGATTGTGGTAATGTGGATTGCAGTTCCTTGTTTCTGTTGTGGAACTGCAATATTCTGAGCTTAAGAT
TGTACTATATTATATTAACATGAAGGCTAATTTTCCGTGATAAAATAACACAATTCAAATTGCTTTAAGAGACTCTACAATTTACATTTTATTTTGAATT
TGAGACTATATTAGACAAGTTTATAATTTAATTTTATGATTTTTTTAGAGCTTCCTTGAGTGCTTTTGGAGAAGAGAAGGACAGTATCTTAAAAAAAATA
AAAAAAAATTAAATGTAAATCAGTTATCCAAATGAGAACAGATTTTTTTTTTTAAGCAAAATATTCAACATTAATAGCTGCAGAAAACAAACTCATCTCC
ATGGATTTCCCTGAGCAGAACAAGCAAACGCTATATATGGAGGTCTTTACTTAGTAATCAGGGTTTACTAGAGGTCTAGATGTCTTTACCACATTAAAAC
ATTTTCACAGCTGGAAACACGTTATACATTCTTCTGTTTTGTAATAATTTGGATGTAAGAAAAAAGAAAAAACTACCAAAGTTTGAACTTAAGTGCAGAA
ACTCTTGGATTGGTGCTGTTTATTAAGGAAGCGTTGCAGTACCCCAGAAAGCCATGTGAGTTTTCATGGGTCTGGAAAGCGTTATTTAGTGCCGTTATTT
TCTTAATTTGAGAATTAACCTATGCCATTTTATTCTGTATGCTCTATGATACATAATAAGTACATATGCTAATCACCTGAACAATATTCTGTTGGATCCT
TTTTGTTAATAAAATTGTGTATATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: BF426650 [+]ggaattcccttgtctacgctgctctaccacgtgtttggccgctagatgctaaggttcctgtcgcgagtcgcattttgcagttacaggcatcttgtttttg
gttatttgaaaccctttcatcgtgccttaaaagcaatggaatccatcgaaatggattctgtttctctaaaacggcctatttctagcaaagaccttagtgg
tgattctgtaaaaaggcaaaaagtctcggacaatttgttctcaacagagacttctttaaaagcacaggatgtggaacctacttcagggattcagaatgaa
aatgccttagatagagaacttacacttgaagtgccaggagagaaaaatgaagaagattttgagaaagacgagcttctggatggggaaactgaagaagaaa
gtccagagagctttgcagacatgatgaagcatggccttactgaaattgatgttggcattactaaatttgtaagcactcatgaagggttttctggaattct
gaaagaaagatactcagactgtgttgtcaatgaaatagggaaagaaggcaagatccttcatttaaatgatgtttcagtaccagcagatgaagggatc
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. 500,000 unamplified cDNAs were mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine), also referred to as Xenopus_laevis_unfertilized_egg_cDNA_library. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: