XB-CLONE-2341595
Clone Name: IMAGE:3746938
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Gene:
dlat.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC099043 [+]
CCTGAGAGGCTTGGTCGGGTCTCCGGCAAATGTTCCACTGCTAAAGGGAGCTTGGAGACAGGGAACGGCCTCTGGAAAGAGATGGTACAGCCTACCTCCC
CACCAGAAGGTGCCTTTGCCTGCCTTGTCTCCCACAATGCAGATGGGAACCATAGCTCGCTGGGAGAAGAAAGAAGGTGATAAAATTAACGAAGGGGATC
TAATAGCTGAGGTTGAAACTGACAAGGCCACTGTCGGTTTCGAAAGTCTGGAGGAGGGTTATATGGCTAAAATCCTTGTGGCAGAAGGAACAAGAGATGT
GCCCATTGGTTCAGTTATTTGTATTACTGTGGACAAGGCAGAATTTATTGATGCTTTTAAAAATTACACATTGGACTCCGCAGCAGCAGCTTCTCCAAGT
GTGGCAGCTGCTACCCCATCACCACCTCCTCAGTCTGCAGTTCAAGCCCCTGGTAGCACTTACCCCAACCATATGAAAATTTGTCTGCCTGCACTTTCTC
CGACCATGACCATGGGTACAGTGCAAAAGTGGGAGAAGAAAGTTGGTGAGAAGCTCAGCGAGGGAGACCTTTTGGCTGAAATAGAGACGGACAAAGCTAC
CATAGGATTTGAAGTGCCAGAAGAAGGTTATTTGGCTAAAATTCTTGTCGCAGAAGGCACACGTGATGTACCCTTGGGCACACCTCTTTGCATTATTGTG
GAGAAAGAGTCTGATATTAGCAGCTTTGCAGATTACAAAGAGTCAACTGGAGTAGTTGATATTAAACCTCAGCATGCACCTCCCACTCCGACTGCAGCTT
CAGTGCCTGTTCCTCCAGTAGCAGTGTCTACCCCTGCTCCTACACCCAGTGCTGCTCCCTCAGCTCCCAAAGGAAGGGTGTTTGTAAGTCCTCTTGCCAA
AAAGCTAGCTGCTGAAAAAGGAATTGATATAAAGCAAGTGAAAGGAAGTGGGCCTGAAGGAAGAATTACAAAGAAAGATATTGACTCATTTGTTCCTCCC
AAAGCTGCTCCAGCTCCAGTTGCAGCCCCAACACCTGCAGTTGCTGTACCATCTCCAGCTGTGGCTGCTGTTCCCAGTGGAGTCTTCACAGATGTGCCCA
TTAGTAATATTCGCAGGGTCATCGCTCAGCGACTCATGCAGTCCAAGCAAACCATACCACACTACTATTTATCCATTGATATAAACATGGGGGAAATCAC
ACAGTTGCGAAAAGAACTCAATGAGGTTACCAAGGCTGACAACATTAAACTGTCATTTAATGACTTCATTATTAAAGCATCAGCTCTGGCATGTTTGAAG
GTCCCAGAAGCAAACTCCTCCTGGATGGACACTGTAATTCGACAGCATCACGTGGTTGATGTAAGTGTGGCGGTAAGCACCCCAGTTGGTCTAATAACTC
CTATTGTGTTCAATGCCCACATCAAGGGGCTGGCATCCATCAGCAAAGATGTGCTGTCTCTAGCAACCCGGGCACGAGAAGGCAAGCTTAAACCTCATGA
ATTTCAAGGTGGAACCTTCACTGTATCCAACTTGGGAATGTATGGAATCAAAAACTTCTCTGCCATAATAAACCCGCCCCAGGCCTGCATCCTTGCTGTA
GGAGGGTCAGAAAATAGACTGATACCTGCAGACAATGAAAAAGGCTTTGACGTTGCCAGTGTGATGTCTGTTACTTTAAGCTGCGATCACCGTGTTGTGG
ACGGGGCAGTTGGAGCACAGTGGCTTGCTGAATTCAAAAAATTCTTGGAGAAACCAACAACAATGCTGCTCTAAATACAACACCTTCCACCTTCAGAGGA
TCAACAATTGCATATCCGAACAAGATATTTATATACGCCTGTCTGCGATGACTCCTAATAAACATGCATCATTCTTTTTTATTTAAAAGAACAACTGCAA
AAAATAGCACCTTGTTTTTTTATTCTCTTAACTGTACAGAGCCATTGTTTCACTTCGGTAACTGTGTACATTTTTGACTGCAAATGCTGTGTTACATGTA
AAACTGTCATCGATATACTAAAGATCTCCTTTTAAAGGTCAACATTTCCTTAACTTGACTGGAAAATAAATCTAGGGTCAGATTAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAA
5' EST: BF427970 [+]cttggtcgggtctccggcaaatgttccactgctaaagggagcttggagacagggaacggcctctggaaagagatggtacagcctacctccccaccagaag
gtgcctttgcctgccttgtctcccacaatgcagatgggaaccatagctcgctgggagaagaaagaaggtgataaaattaacgaaggggatctaatagctg
aggttgaaactgacaaagccactgtcggtttcgaaagtctggaggagggtgtatatggctgaaatccttgtggcag
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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