XB-CLONE-2333238
Clone Name: IMAGE:3747187
|
Gene:
chaf1a.L
|
Species: Xenopus laevis
|
|
|
Sequence:
Full length sequence: BC097864 [+]
CGTCGGATCCTGTACTAGGTTCGAACATGCTTGAAGGGCACATAAAAGACATTGGTGTACCAGAGCGCAAAAAGTTTGGGAGAATGAAATTACTACAGTT
TTGTGAGAATCACCGACCTGCCTACTGGGGCACATGCAATAGAAGGAGCAGGGTAATCAACTCACGAAAGCCATGGGCTCAGGACACAGGCATGCTAGAC
TATGAAGTGGACAGTGATGAAGAGTGGGAAGAGGAAGAGCCAGGGGAGTCTCTGTCACACAGTGAGGGGGAGAATGATGATGATCCCAAAGAAGATGACG
AAGATGATGATGGCTTCTTTGTTCCTCATGGATACTTATCTGATGATGAAGGGGTATCTGATGAGGAATGTACAGATCCAGAGAACCAAAAATTTAGACA
GAAGCTGAAGGCTAAGGAGTGGTATGAGTTGCAAACAAACGGCAAGAAGATCCGTGCAATGCAGCCTGTAGTGATTGGTTGTGTGTGGTGGGACAGTAAA
GCTTCAGAGATTAGTCTTCTGCAAAAATTTTCTGCCTGTATTCTGGAGTCACCTGCAGTTGATGAAGAGCTTGCTCAAGAGATCAGCTCTGCTCAAAGCC
TTAAGGACAGGCAAATACTCTCCAAACTTGTTCCTCTACTTCATGGGAATGTCAATGGAAGTAAGATAATGATACAAGAATTTCAGGAGTACTGTCGCCG
GGGCCTATTCTTGGAGGATAATGCAAGTGATGCTGCAGGTAATGAGTCCACCAGCCCCAACGTCACACCGCAAACACCAAGCAATATTATAGTCCCTTCT
AAAGCTCGCTTGAAGCGATTAATCTCAGAAAACTCAGTGTATGAAAAGCGCCCAGACCACCGTATGTGTTGGTATGTGCATTCTGATGTCTTAAAGGGCC
TTCAACAAGACAATCTTCCTGTACCCTGCCAGTGGACTTATATCACTCAAGTTAACTCTGTAGCCAAGGAAGATAATGGTGCAAATGGAGGATCATTACA
ATCCCTGCCTTTGTCTGGCAAGAGGAAGTCTGCAGGGAGCATGCCAATCACAAAGTTCATGAAGAGAGCAAAGGACCTTGAAACGGCTATAAATACAGAT
ATGGATGGCTTCCAGGCAGACAATGAAGAGGATGATGATGACTGTATGATTCTAGAGGACCAACAAGCTAAAGATGCAGAGGACTCTACCATAGAATGCA
AAATAAACCTGAATGATTCTGCTGTCCTTGCCTCCTGCCAAAACTAAAATAAAAATTAAGCAATGCGTGTATATGTATGTAGATTTTTATATATAAAGAT
TTTTAAAAAAACTTGTGGCAGTTTGAAACTGTAAATTAGCCTTCTGTGTACAGATTGCACTTTCATCTGCAAGATTATTAAAAAGAAATCCTTAACCCAA
ACTGTAAGAAGACCACCAATTAGTTTCTAATAAAGCATACTTCAAATGAAAAAAAAAAAAAAAAA
5' EST: BF614880 [+]tgtactaggttcgaacatgcttgaagggcacataaaagacattggtgtaccagagcgcaaaaagtttgggagaatgaaattactacagttttgtgagaat
caccgacctgcctactggggcacatgcaatagaaggagcagggtaatcaactcacgaaagccatgggctcatgacacaggcatgctagactatgaagtgg
acagtgatgaagagtgggaagaggaagagccaggggagtctctgtcacacagtgagggggagaatgatgatgatcccaaagaagatgacgaagatgatga
tggcttctttgttcctcatggatacttatctgatgatgaaggggtatctgatgaagaatgtacagatccagagaaccaaaaatttacacagaagctgaag
gctaatgagtggtatgagttgcaaacaaacggctagaagatccgtgctatgctgcctgtagtgattggttgtgtg
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: