XB-CLONE-2327987
Clone Name: IMAGE:3747126
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Gene:
gpr4l.2.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: AJ635202 [+]
CCTTAGAATGGCTCTCAGTGGCTGGCTCTGATGTGCTGTGAAGTCTGGAGCAGAGGAGCTTCATTTGGGTCTGGTCTCAGAGGAACTGAAGTGCAGCTGT
TCCCACTGGAAGAATGTGTAACCAGAGCGTGTCGTGTTCATTCTCTGACAATTATGAAGCCGTCTTATTCCCCATAATCTACAGCAGTGTCTTTATAGTT
GGTTTGCCGAGTAACCTAATAGCTATTGGAGTTATTGTTCAACTAATCAAGAAGGGAAACATCTTGGGCATCTACCTGGCCAACCTTTGTGTATCAGACC
TCATGTACATAAGTACACTTCCTGTCTGGATTGTTTACACCGCAAAGGAGGACTGGCTATTTGGTACCCTCAGCTGCAAAATTGTGGGCTTTTTCTTCAA
TGCCAATCTTTACTCGACTATTTCCTTTCTTAGCTGTATAGCTACAGACCGGTTTGTTGCCATGGTTTTCCCACTCCGTTCAAGGATCATCCGGAGCATG
AAAACTGCTGCTGTGATTTGTGTGATAGTTTGGCTGATCATTTTGGGCTCGCATTTTTATTTTTTAACCAGGGACGATGTTTTTACTTCTATCCAGAATG
TGGAACTTTGTTATGAGAAATATCCCATGGAAAGATGGGTGGCTCCTCTGAAATACTTCCGGATTCCTGGGGGTTTTCTTATCCCATTGTTTCTCTTGTT
ATTTTCCTACTGCTCCATAATTCGAGTCATCTATCGAGCAGCCACGTTGGAAACAGATCAGAAGAGGAAGATCACTGGGCTGCTCATGTCTATGACTGCA
ATATTTGTGGTCTGCTACTTGCCGTATCATGTAGTCCTTTTTATCCGTTCCTATGTGAGTGACTTTGACTACTGCAGCTGTACAATAGAGGAGCGTATTC
GCCCTGCCTACCGGATCTCCTTTGCTCTCACCAGTCTGAGCAGTGCCCTTGACCCTTTCCTAAATATCTTTGTGAGTGAAGGAGTAAAGCGGGAATTGAT
GGTGAAAATAAGGGCTCTTTGGTTTTACCTGGTATTCCGGCGAAAAGAAAAGAGCAAAATTAGATGTCTTAATGATTTATGTGAAGGTAATGTTCAGAGG
AACAGTGGCCTCCTGTGTACACACCAAACCAAAATACAGTCTAGGTTGTAGGTAGTTTTTGGGACTAGAAATTTTCTGATTCTGGTAATGGACATTGGAC
TATACTCCTGAAAGGCTGACCTCAAATGTATTAGTGTTCCCCAAGGAATTAAACATCTTTCAGAAATGGAATGTGCAGCAATGACCAACACATGCAACCC
AAACCCTTGAGCTTCAGCTTTGATCAACTTGCTCGCACCTTCTTTGAGTTGAGTTATGGAAACAATGGCCTTGGTCCTCAATAAGGATAAACAAAATTGG
AAAATGTGGATGGGCACAGGCCTTAAACTTGGATTTTCTATTCACAAAGAGACACTGATCCTGCTGTGCGGGAAAATTATATTATTTAGTATTAACATTA
GTATGGAAATAATAAGAATATACCAAATAGTATGTTGTATAATAAATTGTTGTATTGATAACAAAAAAAAAAAAAAAA
5' EST: BF614739 [+]ggaattcccttagaatggctctcagtggctggctctgatgtgctgtgaagtctggagcagaggagcttcatttgggtctggtctcagaggaactgaagtg
cagctgttcccactggaagaatgtgtaaccagagcgtgtcgtgttcattctctgacaattatgaagccgtcttattccccataatctacagcagtgtctt
tatagttggtttgccgagtaacctaatagctattggagttattgttcaactaatcaagaagggaaacatcttgggcatctacctggccaacctttgtgta
tcagacctcatgtacataagtacacttcctgtctggattgtttacaccgcaaaggaggactggctatttggtaccctcagctgcaaaattgtgggctttt
tcttcaatgccaatctttact
3' EST: BG018027 [+]tttgttatcaatacaacaatttattatacaacatactatttggtatattcttattatttccatactaatgttaatactaaataatataattttcccgcac
agcaggatcagttctctttgtgaatagaaaatccaagtttaaggcctgtgcccatccacattttccaattttgtttatccttattgaggtccaaggccat
tgtttccataactcaactcaaagaaggtgcgagcaagttgatcaaagctgaagctcaagggtttgggttgcatgtgttggtcattgctgcacattccatt
tctgaaagatgtttaattccttggggaacactaatacatttgaggtcagcctttcaggagtatagtccaatgtccattaacagaatcagaaaatttctag
tcccaaaaactacctacaacctagactgtattttggtttggtgtgtacacaggaggccactgttcctctgaacattaccttcacataaatcattaagaca
tctaattttgctcttttcttttc
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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