XB-CLONE-2322414
Clone Name: IMAGE:3746813
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Gene:
utp18.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC097862 [+]
CCTCAGGCGCCGTAGCTTCCGGTGGCCTGTAGGTATTGCGGAAGTACTTTAAACTTTAGAAAGTGCACAAAAACGTGTTGAGTCGCAGCGGGTTGAAGTA
AGCTATACGAATATACAAGTGTAAGCGCACTCGAAAGATCCTGCAGTTGAAATGCTTTCTGTTGAGAATACAAATGAAAGCAAAAATGCTTCTTATAAGA
AAAGACAAAGAGATAAATCTCCAGAACAAGATCAAAACTTGAGAGAGGAGGAGGAACTGAGAGCCAAACATTTAAAGGCTTTGGCAGACAAACCAGAGAC
AGAAATGATTTTAGAAGAATTCATTTTTGGGGGTGAAGATGAACTTGTGGAAAGGCTTATTGGTCATTCAAAGGCAAGGCATACTGTAAGTGGAGATCTT
CTAGAGAGCGACTCAGAAGAAGAGACAAGTAAGAATGCAGCACCTTTGGCAAGGAAACCAGCTTGGGTGGATGAAGAAGACGAGGATGAAGAAAATGTTG
AGATGACTCACCGATATCGCAAAGATATGATGAAAAGTAAATCTGAAAAGATTCTTACCAAGGAAAAGCTTCAAACTAGGCTGCAAGAACAGTTTCAGAA
AGCTATGGGAGGAGTGCCTTCCTGGGCTTGCCAAGACCGTAAGAAGAGGCAATCAAAAGAAGACGAGGACAGTGAAGAAGAAAATGAAGATAACTTGCTG
AGTAAGACTGGTAATTTACTGGCCCAATCAAGTTACCTTCCTAAAGGTATTATTCAGATAAGACAATGCTTGAATGCAAACAATGAAAGGATGTCTCCGG
TACCGCTAACAACTGTCCAGTTTCACCCATTGGCACAAGTTGTCATGACTGCAGGATTTGACCGTTCAGTATCATTATTCCAGGTTGATGGAAAGCGCAA
TCAGAAGATCCAAAGCATTCATTTGGAAAAGTTCCCCATTTACAAAGCACAGTTTAGTGCTGATGGCGAACAGGTTATAGCTACAGGACTTCGAAGTAAA
ATGTTTTACATATACGACATGATGGGAGGAAACATTATTCCTGTGCCCAGGATTAGAGGCCTCGAAGAGAAAGCCATTCAGCAATTTGAAGTATCTCCAG
ATGGCTCTATTCTCCTGCTAAACGGATCAGCAGGGTACTTACATTTACTGTCTATGAAGACAAAGGAGCTTATAGGAAGTATGAAAATGAATGGGAAGTC
TGTGAAAGCTGTTTTTTCTTCAGATAGCAGCAAATTGTTTTCAAATTCAGATGATGGATTTGTGTATGTTTGGGATGTGAAAAGTAGGAGATGTCTGAAC
AAGTTTACTGATGATGGCAGTATATGTGGCACATCAATTGCTTTGTCTAGAGATGGGCGATATGTATCATGTGGGTCAAACTCTGGTGTTGTTAATGTAT
ACAACCATGAAGACTGCTTGCAGCAAACAAACCCCAAGCCACTGAAAGCTCTAATGAACCTGGTGACAACGGCTTCATCGCTAATTTTCAATCCACAAAC
TGAACTTTTGGCAGTGGCCTCCAATAAGACTGATGATGCTGTAAAACTGGTGCATATTCCTTCCTTTAGTGTTTTCTCAAACTTTCCAGTACAACATAAA
AAATCCATTCATCTGCCCCGTGCAATGGACTTCTCACCACGGAGTGGCTACTTTTCTATAGCCAACAACAAAGGCGAAGCTTTATTGTACAGGTTGAAAC
ATTATTCTGATTTCTAAAACAGAATATTTAAAGGGTCATGAGCATGAAGATACCTGCTTGGACAGAAATTTCATATGGAGCTAGTGTGAACTTCTTTGAA
TATACCATGTATATTGAGCATTAAACTGTAATGTTTTATACACTTGTTAACAAGAAGAAATAATTAAATAGGTCTTTGTTCTAAAAAAAAAAAAAAA
5' EST: BF427818 [+]cgccgtagcttccggtggcctgtaggtattgcggaagtactttaaactttagaaagtgcacaaaaacgtgttgagtcgcagcgggttgaagtaagctata
cgaatatacaagtgtaagcgcactcgaaagatcctgcagttgaaatgctttctgttgagaatacaaatgaaagcaaaaatgcttcttataagaaaagaca
aagagataaatctccagaacaagatcaaaacttgagagaggaggaggaactgagagccaaacatttaaaggctttggcagacaaaccagagacagaaatg
attttagaagaattcatttttgggggtgaagatgaacttgtggaaaggcttattggtcattcaaaggcaaggcatactgtaagtggagatcttctagaga
gcgactcagaagaagagacaagtaagaatgcagcacctttggcaaggaaaccagcttgtgtggatgaagaagacgaagatgaagaanatgttgagatgac
tcaccgatatcgcanagatatgatgaaaagtaaatctgaaaagattcttaccaaggaaaagcttcaaactaggctgcaagaacagtttcagaaagctatt
gggagagtgccttcctgggct
3' EST: BG021864 [+]ttagaacaaagacctatttaattatttcttcttgttaacaagtgtataaaacattacagtttaatgctcaatatacatggtatattcaaagaagttcaca
ctagctccatatgaaatttctgtccaagcaggtatcttcatgctcatgaccctttaaatattctgttttagaaatcagaataatgtttcaacctgtacaa
taaagcttcgcctttgnngnnggctnnnnaaaagtagccactccgtggtgagaagtccattgcacggggcagatgaatggattttttatgttgtactgga
aagtttgagaaaacactaaaggaaggaatatgcaccagttttacagcatcatcagtcttattggaggccactgccaaaagttcagtttgtggattgaaaa
ttagcgatgaagccgttgtcaccaggttcattagagctttcagtggcttggggtttgtttgctgcaagcagacntcatggttgtatacattaacaacacc
agagtttgacccacatgatacatatcgcccatctctagacaaagcaattgatgtgccacatatact
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: