XB-CLONE-2321994
Clone Name: IMAGE:3749443
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Gene:
sin3b.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC134819 [+]
AGTATGTGGGCGCGCATGGGAGCGCGCACGCAAGGTACGGATCATGGGAAACCTTCCTTCATGTAGCACCGGACGTTGGTTCCATTGCTCAATCTTGCAA
ATATTCACAGCTGTTCCTGGTGTCCAAGGGGAATAATTCTGAAACGATTGCTGGATCATGCTTCCCCAAGTCCAAGTGCTTAAGCCTTTCTTCAGACCCT
TTTTCTGAATAAGCCATTTCACTACCGTACCAAAAACATCTTTTCAAAATGGCAAGTGGCGGAGGTGGTGGTAATTGTAAGCCAAATCCAAACTGGAGCA
AACCTCAAGAAAAACTCCCAGTGCACGTGGAAGATGCGCTTTCTTATTTGGACCAAGTAAAAATCCGTTTTGGAAGTGATCCAGCTACTTATAGTGGATT
CTTGGAGATTATGAAAGAGTTCAAATCTCAGAGCATTGACACTCCTGGAGTTATCCGCAGGGTCTCTCAGCTGTTCCATGAGCATCCAGACCTGATGCTT
GGATTTAATGCCTTTCTACCACTTGGCTACAAAATAGAACTTCCAAAAAATGGAAAAGTGGTTGTTCAGACAACCCTGTCTAGCCCCGTGTCATCAGATG
CAACCACTAGCTCTTTGTCTAGCAGTCGTGTAATTGTGCCCCCTGTTCCTGAAAATGTACTCAGTGAGGTGCCTCAGGAGTCTCGGCAGCAGGAAAAGGA
GAAGCCTCAGCCCCCTCCAGAATCTGACTCACTGGAGTTCCACAATGCTATTAGCTATGTGAATAAAATCAAAACTCGGTTTTTAGACCGGCCTGAAATC
TACAGGTCTTTCCTAGAAATCCTCCACACATACCAGAAGGATCAGCTGAATCCCAAAGGTCGCCCTTTCCGAGGTATGTCAGAAGAGGAGGTATTTACAG
AGGTTGCAAGTCTATTTCATGGACAGGAAGATCTGTTGTCTGAATTTGGTCAGTTCCTTCCAGAGGCAAAGAGATCCTTGTTCACAGGAAATGGAGCTTG
TGATCTTAGTGCCAGGAAACTTGAGTACCAGCGGAACCTAGAACAAAGTCGTAAGAGGTCTAGACCTCTTCTTTTGCGGTCCATGCCTTCTTCCAAGAAG
ATGAAGATGCGAAAGTCCAAGGACCCGTCAATGGCTAGTGTAGGAAAGTATGGCACTCTGCAGGAGTTCTCATTCTTCAATAAGGTCCGCCGTGTTCTTA
AGAGCCAGGAAGTCTATGAAAATTTTCTCCGTTGTATCGCACTTTTCAACCAGGAACTGGTTTCTGGTTCTGAGCTACTGCAGCTGGTCACACCATTTCT
AGGAAAGTTTCCTGAACTCTTTGCACAGTTCAAGTCTTTCTTAGGTGTTAGAGAGCTTTCGTTTGCTTCACCAGTAACAAATGATCGCTCCAATGAGGGC
ATTGGGCGGGAGATCGATTATGCATCTTGCAAGCGCATTGGCTCCAGTTACCGTGCTCTTCCCAAATCTTTTCCACACCCAAAATGCAGTGGGAGGACTG
CACTTTGCAAGGAGGTATTAAATGACACTTGGGTGTCCTTCCCCTCATGGTCTGAAGATTCTACTTTTGTTAGTTCCAAAAAGACTCCATATGAGGAGCA
GCTTCACCGCTGTGAAGATGAGAGGTTTGAGTTGGATGTTGTTTTGGAAACCAATTTGGCTACCATTCGTGTGCTTGAAAGTGTCCAAAAGAAACTCTCC
CGCATGTCTCCTGAAGACCAGGAAAAGTTCAGGCTAGATGACCGCTTGGGAGGCACTTCAGAGGTTATACAACGCAGGGCAATCTACAGAATATATGGGG
ACAAAGCTCCAGATATTGTTGAAAGTCTAAAGAAAAATCCAGTCACTGCTGTACCTGTGGTATTAAAGAGACTGAAAGCAAAAGAGGAAGAGTGGAGGGA
AGCACAGCGTGGCTTCAACAAAATATGGCGAGAGCAGTATGAGCGGGCATACTTGAAGTCTTTAGACCACCAGGCTGTCAATTTTAAGCAAAATGACACC
AAAGCCCTTCGCTCCAAAAGCTTGCTTAACGAGATAGAAAGTGTCTATGATGAGCGGCAGGAACAGCAACCAGATGGCAGGACGACAACATCTAGCGGAC
CCCATTTAATCTACACTTATGAGGATTGGCAAATTCTGGAAGATGCTGCAGCGCTAATAACATTCTATGTGAGTCGGCAACCAACTATCCAAAAGGAAGA
CAAGGACACTATTTGGCAGGTTCTGCACCAGTTCCTTCCTGAGCTGTTTTTCTCATCATCATCATCATCTCCTTCCTGCCAATCTTTATTTGCTGCTTCT
GCCGATGAGACTCCTGTTGAGACTATCCCAGAGTCTTCAGTTACTGAACTGCCAGAACGCAGAAGGAAAGCTGTGACCCCAGAAGTAGTCCCAGAGCCCC
CACCCCCTGTGGAGATACCCCATGAACCTCCTCCAAAGACCATAGATGAAGTTTACAGTTTGTTCTACACCAACAACAACTGGTATTTTTTTCTGCGGCT
ACACCAAACTCTGTGCTCTCGGCTTCTGAAGGTATATCGACAGGCACAGCACCAGCTGATTGAGTACCGGACTGAGAAAAAAAAAAAAAAA
5' EST: BF615431 [+]gcgcgcatgggagcgcgcacgcaaggtacggatcatgggaaaccttccttcatgtagcaccggacgttggttccattgctcaatcttgcaaatattcaca
gctgttcctggtgtccaaggggaataattctgaaacgattgctggatcatgcttccccaagtccaagtgcttaagcctttcttcagacccctntttctga
ataagccatttcactaccgtaccaaaaacatcttttcaaaatggcaagtggcggaggtggtggtaattgtaagccaaa
3' EST: BG022103 [+]ttctcagtccggtactcaatcagctggtgctgtgcctgtccatataccttcagaagccgagagcacagagtttggtgtagccgttgaaaaaaataccagt
tgttgttggtgtagaacaaactgtaaacttcatctatggtctttggaggaggttcatggggtatctccacagggggtgggggctctgggactacttctgg
ggtcacagctttccttctgcgttctggcagttcagtaactgaagacattgggatagtctcaacaggagcttcatcggcagaagcagcaaataaagattgg
caggaaggagatgatgatgatgatgagaaaaacagctcaggaaggaactggngcagaacctgccaaa
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_gastrula_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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