XB-CLONE-2307969
Sequence:
Full length sequence: BC097844 [+]
CCCTTTGTGACCCGCAGGTAACCACATGATCCCATATCATCTGTTGACCCTGAGTAGAAGCTAATTTGTGGGTGACCAACACACCTATGAGTAGAAACTT
CTTTAGATGTCCGTGGTGGACAGGATATTCTCCCTGGGCCCCCAACCCTTCTGTCAGCAGTTGGAACCCTCACGTAAATCGGAGGCCAAGCTATTCCTCG
AGACCTAGTCGAGCTTCCTCTCACAACCCCCTTGGCATTACCTGGAGCACCCAGGCTTCATGTTGGATTCACATCTCAAACCTTAATATGGCTTCTAAAA
GGGACGAACCATTACCAAAACGTATGCGAACTGGAGAGGAAGAACACGTTGAGCAGAATCCGTCTGAGAAGAAGTCATCCAGTCCTTCTAGGGATGCTCG
AGTCCCACCACTACATGCTCCTCCTGAACCTCCCAAAACTTTAGCAGAAGTCCAAGTATCCACTGCCCAGATGCCCTTTACTGCACGTGATGTGAGGAGA
CAATGGGAAGACTTCAATCTATATTATTCAGAATTATCCAGGTGGAATCATGTGTTTGGGAGAAATCCAGAGAACTTTGACTTTACTGTCCTTTCCTACA
ATATCCTTTCCCAGGACCTCCTGGAGGACAACTCCCATCTGTACAGCCACTGCAGAAGGCCCATCCTGATCTGGAGCTACAGGCTGCCTAATATTCTCAA
AGAGCTGGCAGATATGAATGCAGATATACTTTGTTTGCAAGAGGTTCAAGAAAATCATTACAGAACCCAGATCAAGCCCAGTTTGGAAAGTCTTGGGTAC
CATTGTGAGTACAAGGCTCGAACTGGAGATAAGCCAGACGGCTGTGCCATTTGCTTCAAGTCCGATAAGTTCTCCTTGGTGTCTGTGACCCCCGTGGAAT
ATTACAGGCCTAATATAGCACTGCTCAACAGAGACAACATCGGACTTGTGCTTTTGCTTCAACCTAAATCACAAAGAGCAGCCCCTGTAATCTGCGTGGC
CAACACCCACCTGTTGTATAACCCAAGGCGCGGGGACATTAAGCTGGCACAGCTGGCCATACTCCTTGCTGAGATAGCTAATGTGGCATTTACTAAGGAT
AGAGGATTTTGCCCTATTGTGCTCTGCGGGGACTTCAACTCCGTGCCTGGGTCACCTCTCCACAGCTTTATCAGGGAAGGAAAGCTTAATTATGAGGGCG
TGACTATTGGAAAGGTGTCAGGCCAGGAGCAGTATTCCCGAGGGCAGAGGATACTCTCTTGTCCTATATGGCCGAAAAGTTTGGGCATCTCTCAGAACTG
TGTCTTTGAACCAAAGGAAAAGGCAAGGAAGAGCGGCGAATCATTCAGTGAAACATCTGCTGGGAACTCTGCGCCAAATACACAGGTGCAATCTAGCCTG
TACCATCACTTCAGCCTCTCCTCGGTGTACAGTCATTTCTTTCCTGGATCAGGAGTCCCCGAGGTCACTACCTGCCACTCCAGATGTGCCCTGACAGTTG
ATTACATTTTTTATTCAGCAGCAATGAATGACCTATTTGCACAGCTGGGAACGAATTTCAGCCAGAACGGCCTCCAGCTCCTGGGGAGACTCTCGCTGCT
CACGGAACAAGATCTTTGGAGTGTTAACGGCCTCCCTAACGAGACCAACTCCTCCGATCATCTCTCCTTATTGGCCATGTTTAGGCTGGAGATTTGACCT
TATTTTATTTCTTCAGCCTCTTTATTTAATTCCCCTCCCCCCAATCTCGGGATATTTTAATGAAGCGCATAAACAAAAGCCAGTGAAAAACATAGGTTGG
TTTTTATGAAGATTTTTTTTTCTGCCACGGCATTTTATTTGTGACTGAATTTCATTGATGTTCTGTGTGATTTGTAAATTCTCCATTAATGTAAATATTT
TTTAAACAATAGTAAAAAAAAAAAAAAAAAAA
5' EST: BF426511 [+]ggaattcccctttgtgacccgcaggtaaccacatgatcccatatcatctgttgaccctgagtagaagctaatttgtgggtgaccaacacacctatgagta
gaaacttctttagatgtccgtggtggacaggatattctccctgggcccccaacccttctgtcagcagttggaaccctcacgtaaatcggaggccaagcta
ttcctcgagacctagtcgagcttcctctcacaacccccttggcattacctggagcacccaggcttcatgttggattcacatctcaaaccttaatatggct
tctaaaagggacgaaccattaccaaaacgtatgcgaactggagaggaagaacacgttgagcagaatccgtctgagaagaagtcatccagtccttctaggg
atgctcgagtcccaccactacatgctcctcctgaacctcccaaaac
3' EST: BG234451 [+]ttttttactattgtttaaaaaatatttacattaatggagaatttacaaatcacacagaacatcaatgaaattcagtcacaaataaaatgccgtggcagaa
aaaaaaatcttcataaaaaccaacctatgtttttcactggcttttgtttatgcgcttcattaaaatatcccgagattggggggaggggaattaaataaag
aggctgaagaaataaaataaggtcaaatctccagcctaaacatggccaataaggagagatgatcggaggagttggtctcgttagggaggccgttaacact
ccaaagatcttgttccgtgagcagcgagagtctccccaggagctggaggccgttctggctgaaattcgttcccagctgtgcaaataggtcattcattgct
gctgaataaaaaatgtaatcaactgtcagggcacatctggagtggcaggtagtgacctcggggactcctgatccaggaaagaaatgactgtacaccgagg
agaggctgaagtgatggtacaggctagattgcacctgtgtatgtggcgcagagttgccagcagatgtttcactgaatgattcgccgctcttacttgcctt
gtc
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. 500,000 unamplified cDNAs were mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine), also referred to as Xenopus_laevis_unfertilized_egg_cDNA_library. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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