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XB-CLONE-2299026
| Clone Name: IMAGE:4433313 | Gene: itm2a.S | Species: Xenopus laevis | |
Sequence:
3' EST: BG371404 
[+]
Library:
| Name: RIKEN Xenopus egg | External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library |
Description: cDNA oligo-dT primed using oligo 5'-GAGAGAGAGAGGATCCAATACTGGAGAGTTTTTTTTTTTTTTTTVN-3' and directionally cloned into XhoI and BamHI sites using the 5' adaptor 5'- AGAGAGAGAGCTCGAGCTCTAATAAGGTGACACTATAGAACCA-3'. Size selection was done automatically by the cloning vector (lambda FLC-II, P. Carninci et al., unpublished). Library was amplified at the phage stage and normalized (RoT value = 5). This library was constructed using the CapTrapper method and Trehalose thermoactivated reverse transcriptase to enrich for full-length clones. Library constructed by Piero Carninci (RIKEN, Genome Science Laboratory, Japan: http://genome.rtc.riken.go.jp/) using egg RNA supplied by Marc Kirschner (Harvard Medical School, Dept. of Cell Biology). References: Methods Enzymol.303:19-44 and Genome Res. 10:1617-1630 Please contact Marc Kirschner (marc@hms.harvard.edu) for information on obtaining aliquots of this cDNA library.
| Anatomy: egg | Stage: |
| Normalized: No | Subtraction: No |
| Vector Details | Vector Map | Name: pCS2G Type: plasmid 5' Restriction Site: XhoI 3' Restriction Site: BamHI |  |
Usage:
Suppliers:
Search for clone at: Open Biosystems