XB-CLONE-2291186
Sequence:
Full length sequence: BC100231 [+]
CAATTTTAATATTGATGGCATCATTGCAAGGATAGAGAAGACTTTTTCTGGCTTTCCTCTTGAAAGAGCAACTTTGGAAGATATGGGGAAAGTAGCTAAG
GCTTGTGAATGCCCACTGTATTGGAAAGCACCATTGTTTTATGCAGCTGGTGGAGATCGTACCGGATACATTTCTGTTCATAAGTTCATAGCCATGTGGA
GAAAGATCTTGCAGACTTGCCATGATGATTCAGCAAAATTCATGCAGCTTCTTGCAAAACCCGGATGTAATTATTTGGAACAGGAAGACTTCATTCCATT
TTTACAAGATGTAGTGAACACTCATCCACATTTGACATTTTTGAGAGAAGCTTCAGAATTTCATTCCCGCTACATTACCACAGTAGTCCAAAGGATTTTC
TATCAAGTGAATAGGTCATGGTCTGGACGTATCACATGTTCAGAGCTCAGGAAAAGTAATTTTTTACAGCATGTTGCATTACTGGAGGAGGAAGATATAA
ATCAATCTTCAGATTACTTTTCTTATGAACACTTTTATGTTATCTACTGTAAATTCTGGGAGCTTGACACTGATCATGACTTGTACATCGATGAAAACGA
TCTTGCCAAGCACAATGATAATGCAATATCAAGTCATGTTATTCAAAGAATATTCTCAGGCGCAGTAACACGAAGCCGAAAAATTCATAAAGAAGGAAAA
ATTAGTTATGCCGATTTTGTTTGGTTTCTGTTATCTGAAGAGGATAAGAAGACACATGCAAGCATAGAATACTGGTTTCGCTGCATGGATTTGGATGGAG
ATGGTGTATTGTCTATGTATGAGCTGGAATACTTCTATGAAGAACAGTGTCAGAAAATGGAAAGCTTGGCCATTGAGCCCTTGCCTTTTGAGGATTGTCT
TTGTCAGATGTTGGATTTGGTAAAGCCACAAACTGAAGGCAAAATTACTCTCCATGACTTAAAGAAATGCAAGCAAGCAAATATTTTCTTTGACACCTTC
CTTAATTTGGAAAAATACCTTGACCAAGAGCAGAAAGATCCCTTCTCTGTATTGAGGGTTGGAGAAAATGAAGGGCAAGAGCTGTCTGACTGGGAGAAGT
ATGCTGCTGAAGAATATGCCGTCTTGGTAGCAGAGGAAGCAGCAAATGAACAGTGGAATGATGGGTATGACGGAGAGCTGAATATTTTGGATATTCCAGT
GGCAAATAAGGATAAATGTTCAGCTGATAAAAGGTCTTTCTTTGAGTTGCCATCTCATTCATCAGAAGTCGACCTGGAAGAATATGAGTATGAAGATGAC
TTTGAATAACTGTAATGTTTCCCTGCAGCAGGACTGCCATGTTCTCTTTTGGGAATAAGAACAAATTACAAGACAACTGCAACAGCAAGGCTACCTGTTT
TTTTATATATTTAATTTATTACCAAAATAATTATTTATTCATTTTGCGGTGTGATTTCCAGTAATGTTACCATAATATGGCTGAAGTTGTGTTCCTATTG
TTTAATGTCATGTTTCCAAGGCACCACTTGTTTCCATTAGTGCAGTGTTGTTCTTTCATGATATGATTAATTCATCATAATTACCTGTCTGGGAAGTAGT
GCACTGCCTTATGTTACAGAAGTGACTGCTCTACATTTCCCTTGGTAATTAAAGGGCCATGACACAATACGTTTTCTCAAAAAATACTGTAGCATTTGGT
GTCGGTCAACAGTGCCTATACATTCGAGTCATAGAGGTGCCAGAGGATTTCTGTTGCTTCACATGTGTCAACACTGGCGATTTTGACACCAATTCGAAGG
CACTTTTTGATGTTTTGACATCTAAGATGGAGGATATGTAGCGCGGGCGGAAACACACCATGTCCATTGCTTGTCCATCTACTAAGGTCCATGTAACAGT
TTTAATACTAGAATTTTAATCATCCGGTTTAGATGTCACATTCCATTATTTACAAGTAATGCCAACAGGAAGCCGTATACATAGCCTTATACATAACCTG
CTCCACCAGTATATGACATGTTTGTCTGAACTCCTTCCTATTTTTTCTAAATGTACATAATGTAAATTGTGACTTTCAGCATTTTATAACAAACTTTCAT
TTGGAGGGGGGAATATTGTAAATGTTTGCTGTGTACAAAAAAAAGAAATTTAAAGAATTTAAATTCTATAATGTATTTGGAAATAAAATATTTTTTGCAC
AAAAAAAAAAAAAAAA
5' EST: BG487331 [+]ttgatggcatcattgcaaggatagagaagactttttctggctttcctctgaaagagcaactttggaagatatggggaaagtagctaaggcttgtgaatgc
ccactgtattggaaagcaccattgttttatgcagctggtggagatcgtaccggatacatttctgttcataagttcatagccatgtggagaaagatcttgc
agacttgccatgatgattcagcaaaattcatgcagcttcttgcaaaaacccggatgtaattatttggaacaggaagacttcattccatttttacaagatg
tagtgaacactcatccacatttgacatttttgagagaagcttcagaatttcattcccgctacattaccacagtagt
3' EST: BG022040 [+]ttttgtgcaaaaaatattttatttccaaatacattatagaatttaaattctttaaatttcttttttttgtacacagcaaacatttacaatattcccccct
ccaaatgaaagtttgttataaaatgctgaaagtcacaatttacattatgtacatttagaaaaaataggaaggagttcagacaaacatgtcatatactggt
ggagcaggttatgtataaggctatgtatacggcttcctgttggcattacttgtaaataatggaatgtgacatctaaaccggatgattaaaattctagtat
taaaactgttacatggaccttagtagatggacaagcaatggacatggtgtgtttccgcccgcgctacatatcctccatcttagatgtcaaaacatcaaaa
agtgccttcgaattggtgtcaaaatcg
Library:
Description: cDNA from 2 ug polyA+ mRNA, oligo dT-primed using 5'-ACTAGTGCGGCCGCCTAGGCCTCGAGTTTTTTTTTTTTTTT-3'. Library was mass excised (from lambda-ZAPII) using ExAssist phage. The resulting single-stranded phagemids were used to infect TOP10F'. This library has been used successfully to clone a number of full-length cDNAs ranging in size from 1.4-4.5 kb. There are <0.5% clones with multiple inserts. One should be suspicious of any clone which gives 3 or more bands in an EcoRI/XhoI digest AND has an internal XhoI site. Library constructed and donated by B. Blumberg (University of California, Irvine); also referred to as Xenopus_laevis_ooctye_non_normalized. Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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