XB-CLONE-2210003
Clone Name: IMAGE:5162445
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Gene:
tmx1.L
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Species: Xenopus laevis
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Sequence:
5' EST: BQ398284 [+]
gggcaagtcatcttgtgagaagcttaggatttcgcagtcagctgtgctgcacattacaattgtctgaggtacaaatgttatacttttgtcacttttcgtt
actaggtcagaacagatgtcacagatccttgcactccatacacagaaacaattcattatgaccgctttcaagaattctgcctgttctccaatttattcca
actccctacagtaatttaggtatgatatccttctcattgtggtgcattaatgcaatcttcccctgttttccttgcatgaaatcactgatttaataaagga
aatgggctttgcaaagattactgttactaacgtattttccatacagttattgtaattacaatacctgtaacaccttaggggcatcaagatgcattataag
tactaatgctttaaagcataaatatggtttgggttaaaatcaaattgttggccaaaaaagaattttcccaggcaatgaaaaactgcaataataatgtatg
aatagatgtacagtaggtgaagagagccaatggagtttagggggcttaacttgagttgctttttacagctttacagaacagagggaacagcaaagctgca
tcatgactacctgcaaactgctgaaatgtgatgtcag
3' EST: BQ398283 [+]aattcaccaagaaagcaaaaacagtttattcattgcagcatgttacaaaaatacaaaatatattttcaagcaaactgatttacaaagactgtgcagttta
gctttgcaaaaagtgtttcctgaacttatactaattttccccaaaaccagaatctaaatgaaagctgttatatggtatcattatacaacgcaaattcaac
actggatcccatatgcgttatttggctcttcagaaggtaaaaacaaaaagacgacaagaattattaactattttgcattgttaatggtctacagtgctat
caataataaagtaaaagaacgtctgtgcataccctgtaacaactgtccaacacctggtagtcttataattaagctactctgacatcacatttcagcagtt
tgcaggtagtcatgatgcagctttgctgttccctctgttctgtaaagctgtaaaaagcaactcaagttaagccccctaaactccattggctctcttcacc
tactgtacatctattcatacattattattgcagtttttcattgcctgggaaaattcttttttggccaacaatttgattttaacccaaaccatatttatgc
tttaaagcattagtacttataatgcatcttgatgcccct
Library:
Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa). Vector Details | Vector Map |
Name: pT7T3D-PacI
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: NotI | |
Usage:
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