XB-CLONE-2192914
Clone Name: tubb2b-733
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Gene:
tubb2b.S
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Species: Xenopus laevis
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Description: EXRC Clone Number 733. Xenopus laevis tubulin, beta 2B class IIb. Image EST 5571885, full length cDNA. Accession AAH44030. To generate probe for in situ.
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Sequence:
Full length sequence: BC044030 [+]
GTTCTTAGCCGGCTGGAAACCATCGGGGTGTTGCACAAAGGGCAAGTAAAGCAACAAGAAAGGGCCACACCATCCACTGATTACACCACTGTAAGACTCA
ACCCATCGGAAACATGCGTGAAATCGTGCACCTCCAAGCTGGCCAGTGCGGTAACCAAATTGGAGCTAAATTTTGGGAAGTCATCAGTGATGAACACGGC
ATTGATCCTACCGGCAGTTACCATGGAGACAGTGATTTGCAACTAGAAAGGATTAACGTATACTACAATGAAGCCACAGGTAACAAATTTGTACCCCGTG
CCATCCTTGTGGATTTGGAACCAGGCACAATGGACTCTGTCAGATCTGGACCATTCGGGCAGATTTTCAGACCAGACAACTTTGTGTTTGGTCAAAGTGG
TGCTGGCAATAACTGGGCCAAAGGTCATTACACCGAAGGAGCTGAGCTGGTGGACTCTGTTCTAGATGTGGTGAGAAAAGAATCTGAGAGCTGTGACTGC
CTACAAGGTTTTCAACTGACCCATTCTCTGGGTGGTGGCACAGGCTCTGGTATGGGTACCCTGCTCATCAGTAAGATAAGGGAAGAGTACCCAGACCGAA
TCATGAATACATTCAGTGTGATGCCATCACCCAAAGTCTCAGACACTGTGGTTGAACCATATAATGCAACCCTCTCTGTTCATCAGTTGGTGGAAAATAC
AGATGAAACCTACTGCATAGACAATGAGGCCCTCTATGATATCTGCTTCCGCACTTTAAAGTTAACAACACCAACATATGGTGATCTGAATCACCTTGTA
TCCGCTACAATGAGCGGGGTAACAACCTGCCTTCGTTTCCCAGGGCAGCTTAATGCTGATCTACGCAAACTGGCTGTCAACATGGTGCCTTTCCCTCGAT
TGCACTTTTTTATGCCAGGCTTTGCCCCATTAACAAGTCGTGGCAGCCAACAATACCGAGCCCTGACAGTGCCAGAACTAACACAGCAAATGTTTGATTC
CAAGAACATGATGGCAGCGTGCGATCCCCGTCATGGACGCTACCTCACAGTAGCTGCTATCTTCCGTGGAAGAATGTCTATGAAGGAGGTAGATGAACAG
ATGCTCAATGTCCAGAACAAGAGCAGCAGCTACTTTGTTGAATGGATTCCCAACAATGTGAAGACCGCAGTTTGTGACATTCCACCAAGAGGCCTCAAAA
TGTCTGCAACCTTTATTGGTAACAGCACTGCTATTCAAGAGCTTTTCAAAAGAATCTCTGAGCAGTTCACTGCCATGTTCCGTCGCAAAGCTTTCTTGCA
CTGGTACACTGGTGAGGGCATGGATGAGATGGAGTTCACAGAAGCTGAGAGCAACATGAACGACTTGGTGTCCGAGTATCAGCAGTACCAAGATGCAACA
GCTGATGAGCAAGGCGAGTTTGAGGAAGAGGAAGATGAAGCTTGATGACAAAGGCTCCTAGCATTTTTAGAAATAAAAAGGCACAGTTTTTAAATAGCTA
GAGCATTTGCTCAGAATTTTGTTTCAGCATGCTTCTTCATTTTCTATTGTCAATGAAACCACAATCAGTTTTTTGTAACCTTTACAATGTCAAAGTAACA
GTTGTTTTTAGCATCTCTTCTAACGTTTACAAAATGCCCAAACATGATTAGGATGATGGTAGAAGTGATTTCGACCACTCATAGATGTTTTCCCCCACAC
TGGATAAGATCATAAAAGTATTTGTGCTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: BQ736857 [+]ggacgcgtgggttcttagccggctggaaaccatcggggtgttgcacaaagggcaagtaaagcaacaagaaagggccacaccatccactgattacaccact
gtaagactcaacccatcggaaacatgcgtgaaatcgtgcacctccaagctggccagtgcggtaaccaaattggagctaaattttgggaagtcatcagtga
tgaacacggcattgatcctaccggcagttaccatggagacagtgatttgcaactagaaaggattaacgtatactacaatgaagccacaggtaacaaattt
gtaccccgtgccatccttgtggatttggaaccaggcacaatggactctgtcagatctggaccattcgggcagattttcagaccagacaactttgtgtttg
gtcaaagtggtgctggcaataactgggccaaaggtcattacaccgaaggagctgagctggtggactctgttctagatgtggtgagaaaagaatctgagag
ctgtgactgcctacaaggttttcaactgaccncattctctgggtggtggcacaggctctggtatgggtaccctgctcatcagtaagataagggaagagta
cccagaccgaatcatgaatacattcagtgtgatgccatcacccaaagtctcagacactggtggttgaaccatataatgcaaccctctctgttcatcagtt
ggtggaaaatacagatgaaacctactgcatagacaatgaggccctctatgatatctgcttccgcacttttaagttaacaacacccacatatgggggatct
gaaatcaccttgtatcccgctaccatgaagcggggtaacaaccctgccttccgtttccccaggggcacccttaaagggttgatcttacccaaactgggct
ggtcaaaaatggggggcccttttccc
Library:
Description: Cloned unidirectionally. Primer: Oligo dT. Average insert size 2.1 kb. Constructed by Life Technologies. Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pCMV-SPORT6
Type: phagemid
5' Restriction Site: NotI
3' Restriction Site: SalI | |
Usage:
Whole mount in situ hybridization:
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Expressed in Primary neurons.
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
SalI |
EcoRI or SmaI can also be used. |
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Suppliers:
Search for clone at:
EXRC