XB-CLONE-2118418
Clone Name: IMAGE:6872626
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Gene:
bbs5.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC081218 [+]
GGGGTTACAAAATGGAAGCAGTTGCCCTGGAGACCGCAGGCTTATAGTCTTGAGCAGTGCAGCCGAGGTTATTAGCCTGAGGTTGGAGCAGTAAACAAAA
TGCTGTCTGTGTTGGACGCGCTGTGGGAGGATCGGGATGTTCGCTTTGATATATCGCCCCAGCAAATGAAAATGAGACCAGGGGAAGTGCTCATAGACTG
CTTAGATTCTATTGAGGATACCAAAGGAAACAATGGAGACAGAGGAAGACTGCTAGTCACCAATTTACGAGTTATTTGGCATTCCCTAGCACTTCCCCGT
GTGAATCTTGCTGTTGGCTACAACTGTATTATAAACATCACGACAAGGACTGCTAATTCGAAATTACGTGGTCAGACTGAAGCACTGTACATCTTAACAA
AATGCAACAATACTCGCTTTGAGTTTATCTTTACAAATCTTGTACCTGGGAGTCCTAGACTGTTTACTTCTGTTATCGCTGTACACAGAGCTTATGAGAC
ATCTAAAATGTATCGTGATCTCAAACTGAGAGGCGCACTCATCCAAAATAAGCAGCTGAAGCTTCTTCCACGTGAACAAGTTTACGATAAAATCAATGGA
GTGTGGAATTTATCCAGTGATCAGGGAAATTTGGGAACATTCTTTATAACCAATGTGAGAATAGTTTGGCATGCCAATATGAATGATAGTTTCAACGTCA
GCATTCCTTATCTGCAAATTCGTTCCATAAAAATCAGAGACTCAAAGTTTGGTTTAGCACTGGTGATAGAAAGCTCCCAGCAGAGTGGTGGATACGTCCT
TGGCTTTAAAATTGATCCAGTTGAGAAGCTACAGGATTCAGTGAAGGAGATAAACTCATTGCACAGGGTCTACTCTGCAAGCCCCATATTTGGAGTAGAG
TATGAGATGGAGGAAAAGCCACAGGCCCTTGAAGAATTAACCATAGAGCAGGTTCAAGATGATGTTGAAATAGAAGCTGATGAACACACTGATGCATTTG
TGGTAAGTCGTCTTTCTAGGATTTCATATTTTACCAGGCAAATCATTTATATATTAATATTTACAAGAATGTATTCTGGATGGTTTCACCACAGCTTGAC
TAAGCTGCATGGTAGCATACGATTTAACAGTGTAACAATTGCTGCAGATTTTCTTTACATATACATATCACAGAATGGGACAAAAATAGTAAAATAAACT
TGTCATTATCTTACATGATTAGTCTCATTCCATATTTTTAATGCGTGTATTGCAATAATTCAAGTTATTTTAAACATGAACAGTTATGCCCTTCCATTCC
ATGTATTTCTCAGAAATTCCATTCCTATACTTAGGGGCAGATTTACTTAGGGTCGAATATCGAGGGTTAATTAACCCTTGATATTCGACTGCCGAATTGA
AATCCTTCGAATATCGAAGTCGAAGGATTTAGCGCAATTCGTTCGATCGAACAATTGAATCCTTCGAATCATTCAATTCGAAGGATTTTAATCAATCGAA
TGAACGATTTTTTCTTCAACCTAAAAATCGCTCAAAAGCCATCAAAGTAGCCCATTCGATGGTCGAAGTAGCCAAAAAAATCATTCGAAATTCAAAGTTT
TTTTTTTTTTTTTTATTCTGTTCCTTCACTCGAGCTAAGTAAATAGGCCACATATTGTGCCTGACCCCAGTACTATGGCGCATGTATCAAAGGGGCAGGG
TCCAAAATGCCCCATTTTTTTTGCACTTGCTTTCTTTTTGGAATTTACTGCTAGTGTCTTATCACCTGGCTTTCACATCGGGGTACAAGGGAACTCTGTA
CTTACCTCCTGCTTATGTTAACTATTAAGTTCTCCCGTCCACATGTCAGAAATGAGTTCTTCGTCGGCGTTAGATTTTCAGTACAGCACAGTGTTGCAGC
TATTTGCACTGTTGCACTAGCACCTGCATTGTGTTAAATATAAACATATTTATGTGTTTATGTTCTCTTTTATAGGCTTACTTTGCTGATGGGAATAAGG
TAGGATATTTTTTCTTTTGCTTAAATATTTACTCTAAAATATCCTGTTTATCTTCCGGACACTTCCCCTGACTCTTTCTTTATTCCAGCAACAAGATCGG
GAACCAGTCTATTCTGAAGAGCTTGGCTTAGCAATAGAGAAACTGAAGGATGGCTTTACCCTGCAGAGATTATGGGATGTGATGGCATAAGGAACGACTG
CAGTTTTCTATGGAATTTAGAGATCTAAGCACAGTTGCACAAATCCTAAACACATCCCCATAAATTGCAGAGTTGTAGCAGTTTTTGCCAGTACATTATA
CATTAGGAGGCACATTTATTAAAGGTCGAATTACGAATTCATGGGAGTTATTTCTAACTCTATCAAATTCGAATATATGCTCAAAATTCGACTGGGGGGT
TATTTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB197726 [+]cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggttacaaaatgga
agcagttgccctggagaccgcaggcttatagtcttgagcagtgcggccgaggttattagcctgaggttggagcggtaaacaaaatgctgtctgtgttgga
cgcgctgggggaggagcgggatgttcgctttgatatatcggcccagcaaatgaaaatgagaccaggggaagtgctcatagactgcttacagtctattgag
gataccaaaggaaacaatggagacagaggaagactgctagtcaccaatttacgagttatttggcattccctaacacttccccgagtgaatcttgctgttg
gctacaactgaattataaatatcacgaccaggactgctaattcgaaattacgcggtcagcactggaaatactgtaacatccttaacaaaaatgcaacgaa
gtactcgccttttgcagctttatcatttaccaaaatccttggaaccctggggaaaattcctaacaactggggttaacctttccgggtataatccggcttg
gtaaacaccaattaaggctattaattgcacgaaccgatccctcaacacaaccgactctctcaccctggcaaatccatctcccataaccttggtacagaaa
acagccccactaaccttcccgataaccccgctatagatttatagagtgcagtggtattgtagaaagcgcgtttatccattttcccagcactaagcgacaa
gctcagagtccattcatggctcactacgaccgtatcttaaatcaggcgaaaaaaggacctgtgaccaatcttctatttccctcaaagcgagaacacccag
ggggacacacgatattgtagggacaaagcacattctctcatacgaaacaaaacccccaccggttagcaaaaacacaaacctttatggccgcttggcaaca
agaatatcgcataataaactaatgcttctctagagtagtcaaaccaaattatccctctcgtactggtcctaattatcactccagcacataagaaattcac
caagaaan
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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