XB-CLONE-2116370
Sequence:
Full length sequence: BC078598 [+]
GGGGCCACGGAAATCCTGTATTCCCAAGTGGATAAACACACCCAAGAGAGTGCAAACAGCAGCACCCTGAACCAGGCTAGAAATGGAGGAAGAAACACTG
CTAACTCTGCTCTGGACAGGACAAATCACCACCAGGTTGGATGCAGAGAGCTGAGATCTACCAAGTACATCTCGGATGGACAGTGTACCAGTATCCAGCC
TTTGAAAGAACTGGTCTGTGCTGGAGAGTGTCTTCCTCTTCCTATTTTGCCCAACTGGATCGGGGGCGGCTACGGGCTGAAATATTGGAGTCGAAGAAGC
TCTCAGGAATGGAGATGTGTCAATGACAAGACCCGCACTCAGCGTATCCAGTTGCAGTGTGAGGATGGCACAACTAGAACCTACAAAGTCACAGTGGTAA
CTTCCTGCAAGTGCAAAAGGTACACCAGACAGCACAATGAATCCAGCCATAACTACGAAGGAGCTTCTCCAATCAAACCCATTCACTCTCAGCAACATCA
TCACTCCCACCACAACCGTGATAAGAAAAGACTAATCAAGATGTCCAAGCACATTCCTAGCTAGAGAAAAGATACAAAACGCTGAACTGCTTCTTTTGAC
TGAATATGTCTCCAAAAGACTGAATTTGTAAATGGAGATGGAAAACCCGGGGGGTTTTTTGTGATAAAGATTGTTCGTATGGAGAACACACATTATAAAT
AACACTTTCATCCTATTTTGTTCCACGTCATTTTGAAGGACTGACGTATCTTTTCCAGAGAATAAAAGGAAGTTTTGTATGTAATACTTTTATTGAAAAG
TAAAAGGTGCTTCAAACAGGTTTAAACAAGTTATGTACTAGAATTGTATCTTAAAATAATTTATAAAGTCCCTCTTCTTGGTATATGGTCCTTATACATT
TGGGCTGACAGCTGAATATTTTGTGGCAAGGAAGTATTGGTTGCTAAAGGGAAAGAGTTTTCATAGATTTTGCTTTAAATGTGTTTAAAGGTAAAAAAAA
ATAACACTTGCATATAACAAGCCCTTTTTAACCTAGGCAGAAAAGTATATTATATTGAACAATAAATCCCCTGTATTTATTAATATAAGTGAGGGCTGGT
CTGTACATGGGATTATTTTTCCTACAAACCAGGGATTTGCAACTGTGGCAACCCAGCCATTGTTTATTGGGGTAACTGGTATAAATCTACTCCCACTACT
GGGAAGTCATGTGCTGCAGACCCCTACTATAGAAAAATGAAGGTCAACAACACATCAGCCAATAATGCTAATGCTAATATTTGTTGAGAGCGCTTGTGTA
AAATGTGCTCACAAACTTTGGTGAATAAAGTCCACTTTTGGGGCCAAATGTTTGTCTTGAAAGGAAAGTATAGCTAAGGGGCAGATTTATCAATGGTTGA
ATTGAAAATTTAGAGTTTTGACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB200202 [+]cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggccacggaaatcc
tgtattcccaagtggataaacacacccaagagagtgcaaacagcagcaccctgaaccaggctagaaatggaggaagaaacactgctaactctgctctgga
caggacaaatcaccaccaggttggatgcagagagctgagatctaccaagtacatctcggatggacagtgtaccagtatccagcctttgaaagaactggtc
tgtgctggagagtgtcttcctcttcctattttgcccaactggatcgggggcggctacgggctgaaatattggagtcgaagaagctctcaggaatggagat
gtgtcaatgacaagacccgcactcagcgtatccagttgcagtgtgaggatggcacaactagaacctacaaagtcacagtggtaacttcctgcaagtgcaa
aaggtacaccagacagcacaatgaatccagccataactacgaaggagcttctccaatcaaacccattcactctcagcaacatcatcactcccaccacaac
cgtgataagaaaagactaatcaagatgtccaagcacattcctagctagaagaaagatacaaaacgctgaactgcttcttttgactgaatatgtctccaaa
agactgaatttgtaaatggagatggaaacccgggggggtttttttgtggataaaaattggttcgtatggagaaaacaccattataaataacacttttcat
cctatttttggtccacgtccatttttgaaaggactggacgtatccttttccaaaanaaataaaaaggaaagttttggtatggtaatt
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
Suppliers: