XB-CLONE-2109108
Clone Name: IMAGE:6880081
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Gene:
med1.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC099369 [+]
ACCCTTCCAAAAAAGGAGGAGGGGATTCTAAGGGCTCTGTGAGCACAGGTGTGGCTAAAATTATTATCAGCAAGCATGATGGGGGCTCTCCCAGTATAAA
AGCAAAAGTGACATTACAGAAACCAGAAGGCGGTGGGGATGGACTTCGATCACAGATGCAAAAAAACTACGGCTCTCCATTAATTAGCGGTTCTACTCCA
AAACATGAAAGATGTTCACCAAGTCATAACAAGTCCCCAGCATATACCCCTCAGGCGCTGGACAGTGAGAGTGAATCCGGCTCCTCTTCTATAGCAGAGA
AGTCTTACCAAAACAGCCCAAGCTCAGACGACGGAGGGGGCAGCGGAAATAGGGCCCAGACTGAGTATAGTGCAGAGAAACACAAAAAGCACAAAAAGGA
GAAGAAGAGGCTCAAGGACAGGGACAGAGAGAAAAAGAAATCTTACAGCATGAAGTCAGAAAGTTGGTCCAAGTCTCCAATTTCCGTTGACCCCACCATG
GTCATGTCTGGAGGTTCCATGCTGAGCTCCGACCGAGGTGTCCGGCCAACTCCAAGTTTCCTCATGGGTGACGACGACTTAATGGATATGTCGCTCACCT
TATAGAACCCCGAAACTTCATCAGTGCTGGACTTTGTCTCTTTGAGGTGCAACTAAAGCTTTGCTTGCCCACTCTGACGGTATCTCCGCTATATTTTTTA
CTACGTTGGTCCTATTTTAAATATATTTAAGTTTTTATTTAAATGTAAAAGCTCCTTTGTCCCAACCGGTTTTACCTATGCCACTGTATAGACACACGGC
CATTTTGCAGTTTTATATATTAAATTTGGGTGCCCAGTTTTTCTTTTAAGTGCTATTTTTTTAATTGAAATTGGGTTTTTTGTCTTTAAAGGGGTTTCAC
CTTTAAACACTTTTTTTTCAGTTCAGTTGGTTTCAGACAGTTCTCCAGAAATAGACTTTTTTTCCAATTACTTTCTATTTTCTATGCGTGACTGTTTTTC
TATTATGTGTGAAGTGTCATTTTTTCACCTACTAAAGCAGCTCTGGGAGGAGGGGTCGCCGACCCTGTAAACTGTTCTAAAGTGATACATTTCTTATGTT
TGTCCCTACAATAGTTATTAATACTCCAGAGATGCTACTGAGAAATCATCGGATGCACCGAATCCAGGATTTGGTTCGGGATTCCGGCTTTTTTCAGCAG
GATTCGGCTGAATCTAACCCTAATTTGCTTATGCAAATTAGGGGTGGGAGGAAAATTGCGTGACTTTTTGTTACAAAATAAGGCAAGTAATAAAAGTTTT
CCCCTTCCCACAACTAATTTGCATATGCAAATTCGGATTTGGCTCGGTATTCGCCCAAATGTTTTGTGAAGGATTCGAGGGTTCGGCCAAATCCAAAATA
GTGGATTCAGTGCATCCCTACTATCAACTAAATGTTGCAAAATTGTAACAGTTCAGAATCTGCTCCAGAATTACTGAGCTGCCGGACTCAAACATCAGAG
ACAGAAATTTAAACTCAGATTTTCGAAAAAATTGTTAAAAAATGGAAGGCAATTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB207654 [+]gatacactcctatagggcgctagctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacatt
atacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggacccttccaaaaaaggaggaggggattctaagggctc
tgtgagcacaggtgtggctaaaattattatcagcaagcatgatgggggctctcccagtataaaagcaaaagtgacattacagaaaccagaaggcggtggg
gatggacttcgatcacagatgcaaaaaaactacggctctccattaattagcggttctactccaaaacatgaaagatgttcaccaagtcataacaagtccc
cagcatatacccctcaggcgctggacagtgagagtgaatccggctcctcttctatagcagagaagtcttaccaaaacagcccaagctcagacgacggagg
gggcagcggaaatagggcccagactgagtatagtgcagagaaacacaaaaagcacaaaaaggagaagaagaggctctaggacacggacacagagagaaag
agatcttacaccatgaagtcagaaagaaagttgggcccacagcctccaattttcccggtgaccccaccatgggtcatggtctggaggttccatggctgag
cctccgcaccgaggtggtccgcgccaactccaagtttccctcatggggggacccaccacttaagtgggatattgtcgctcccccctataaaaacccccaa
aaccttccctcccggggtgggatctttgttcttcttcggagggggcgaaaaaaaagtttttgtctcgccccaccccccgcagggagatccccccnccacc
tttttttttcttcccgggcgggccctggttcctaataaataaaccaaccccctcaccttttaaccggaaaaaagggtccattaccctccatttctggcac
cagcccgggtttctcacctcgacgcccccccggt
3' EST: CB560533 [+]agttaaatttctgtctctgatgtttgagtccggcagctcagtaattctggagcagattctgaactgttacaattttgcaacatttagttgatagtaggga
tgcactgaatccactattttggatttggccgaaccctcgaatccttcacaaaacatttgggcgaataccgagccaaatccgaatttgcatatgcaaatta
gttgtgggaaggggaaaacttttattacttgccttattttgtaacaaaaagtcacgcaattttcctcccacccctaatttgcataagcaaattagggtta
gattcagccgaatcctgctgaaaaaagccggaatcccgaaccaaatcctggattcggtgcatccgatgatttctcagtagcatctctggagtattaataa
ctattgtagggacaaacataagaaatgtatcactttagaacagtttacagggtcggcgacccctcctcccagagctgctttagtaggtgaaaaaatgaca
cttcacacataatagaaaaacagtcacgcatagaaaatagaaagtaattggaaaaaaagtctatttctggagaactgtctgaaaccaactgaactgaaaa
aaaagtgtttaaaggtgaaaccccctttaaagacaaaaaaccaattttcaatttaaaaaaatagcactttaaaaagaaaaactggggcacccaaatttta
atatataaaaactgcaaaaatggccgtgtggcctatacagtgggcataggtaaaaaccggtttgggacaaaaggaactttttacattttaaataaaaaac
ttaaaatatatttaaaaattaggaccaaccgtagtaaaaaaaaatttgccgggagaaataccgtccagaagtggggcaaagcaaaagctcttagtttgcc
ccctcaaaagaagacaaaagc
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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