XB-CLONE-2108026
Clone Name: IMAGE:6879525
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Gene:
apob.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC054229 [+]
GATTCTCTCTTGGGTCAGTGGAGGAGAAAATAATTGGTGTCTGTGGGTCACGGCAGCTCCCAAGAGCTTTAACAGCTCATCATGGGCAACGAGAAGCTGT
TGCTCCTACTTTTGCTAGCCAGTGCCTTTGCTCAGGAAGATGGGTCAGACTCTCAGGATTCAACCTGTTCAAAAGCTGCTTTAAAATTCAAGCCTTTAAG
GAAATATGTCTACAACTATGAAGCTGCAACTACAAGTGGAGTGTCTGGTACAGCGGACTCCCAAAGTGGATCCAAATTCAGCTGCAAGATTGAATTGGAG
GTTCCTCAGCCATGCAACTTTATCCTACGGGTCAAGCAATGCACCCTGCGAGAGGTGTATGGGGTCAGTTCCGAGGGCAAAGCTCTTCTAAAGAAGTCCA
AGAACTCCGATGACTTCTCCAACGCTATGACCAAGTATGAGGTTAAGGTCGCTGTACAAGACGGGAAGAATGTTGTTCTGTACCCAGACCAAGATGAAAC
TCTCACTGTATTAAACATGAAGAGGGGGATCCTTTCTGCTCTGCTTGGTCCTGATGAAACCCAGGAAACAGATTATGTGGATACTGTGTATGGAAAATGT
ACCAGTGAGATTAAAGTCACAAGCCGAAAGGGTAACACACCAACAGATGTTACGGTGGAGAGGAATCTGAAGACCTGTGACAAGTTTACACCAATCAGAG
ATTATGTCAGCCCCCTCGCAATCCTTAAAGGCCTGAATACCCCATTGTCTACACTGATCAGCAGCAGTCAGGTCTGTCAGTACAGCATGGATTCCAAGAG
GAGGCACATCACAGAGGCATCATGTGTTGAAACTCACATTTTCCTTCCCTTCTCACACAAGAATCAGTATGGCATGAATAGCAAAGTCACTCAGACACTG
AAGCTGGAAGAAGTGACTAAGACCAACAGCAGAGATTTTGATCCAGATCCCAGCGTGGCACGAGGCCTTGCTATGGATGCTACAAAAACACCATCCAAAA
ATGAAGAGGCTGCATTGGAACATCTACAGGAGTTACAGAAACTGTCTACGACAGAGCAGAACCAGCAGAGAGCCAACCGCTTCTACAAACTTGTGACAGC
CTTAAGGGGTCTAAATAACGACTCACTAGGACCTCTTGTTCCAAGGCTAATGCAGCTGTCAAGCCCTATTGCTCTTCAAGCGTTGACTCAGTGTGGGACC
CCTGAATGCTTTGGCGGTATCCTTCAGGTGCTCAGAAGTGGAGACATATCCCCAGTCATAAGCGACACAGTTACTTACGGCTTGGGCCTGTTACCTTCTC
CATGCACAAAACGTGTACGGGAACTTCTCAACATGGCCCAGTATCAGTCAAGCAGAGCTTCATTTTATGCATTAAGTCACGCCGTCAGCAAGTAAGGCCT
TTCATTAGTACTTTATAATTCAGTTTTGCCTATCTATATATCTATCTCTCTATTTATCTGTCTGTCTATCTAATATATTTTTGTGTATGTATACCCCCAA
CTATTGAGAATGAACCACTAGTAGTCCCACAAAAATGAATATAAATTTGTATTTATAGTAAGTAATGAGGATTAGACCCTGTATGTAGTGAAAAATAAAA
TTTATGATCGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB207180 [+]gggattctctcttgggtcagtggaggagaaaataattggtgtctgtgggtcacggcagctcccaagagctttaacagctcatcatgggcaacgagaagct
gttgctcctacttttgctagccagtgcctttgctcaggaagatgggtcagactctcaggattcaacctgttcaaaagctgctttaaaattcaagccttta
aggaaatatgtctacaactatgaagctgcaactacaagtggagtgtctggtacagcggactcccaaagtggatccaaattcagctgcaagattgaattgg
aggttcctcagccatgcaactttatcctacgggtcaagcaatgcaccctgcgagaggtgtatggggtcagttccgagggcaaagctcttctaaagaagtc
caagaactccgatgacttctccaacgctatgaccaagtatgaggttaaggtcgctgtacaagacgggaagaatgttgttctgtacccagaccaagatgaa
actctcactgtattaaacatgaagagggggatcctttctgctctgcttggtcctgatgaaacccaggaaacagattatgtggatactgtgtatggaaaat
gtaccagtgagattaaagtcacaagccgaaagggtaacacaccaacagatgttaccgtgggagaagaaatctgaagacctgtgacaagtttaccccaatc
agagattatgtcagccccccctcgcaatccttaaaaggccggaataccccattggtctacacctgatcagccagcc
3' EST: CB561074 [+]ggggtggcgggtgggcgattcgaattataacgcgggcccagaggtgggggggtggnngngngntttccaaaaaggcgggggggcgggctataaattcacc
tttatattcatttttgtgggactacttgtggttcattctcaatagttgggggtatacatacacaaaaatatattagatagacagacagataaatagagag
atagatatatagataggcaaaactgaattataaagtactaatgaaaggccttacttgctgacggcgtgacttaatgcataaaatgaagctctgcttgact
gatactgggccatgttgagaagttcccgtacacgttttgtgcatggagaaggtaacaggcccaagccgtaagtaactgtgtcgcttatgactggggatat
gtctccacttctgagcacctgaaggataccgccaaagcattcaggggtcccacactgagtcaacgcttgaagagcaatagggcttgacagctgcattagc
cttggaacaagaggtcctagtgagtcgttatttagaccccttagggctgtcacaagtttgtagaagcggttggctctctgctggttccgctctgtcgtaa
acagtttctgtaactcctgtagatgttccaatggcagcctcttcatttttggatggtgtttttggagcctccatagcaaggcctcgtggcacgctgggga
tcggggatcaaaatcttctggtgggtggtccttaagtcctttctttccagccttcagtggtctgggtggactttggctaattcatggccaaaacggattc
cttgtggtcgaaacaagggaaaggaaataatgtggtagttttcaaacacaatgggagccctccggtgggagagggaccctccctccttgggagaaccctc
ggctggttaactgaacagaaaccttggaccggccagccggacatcagtgggttagaacaaagagggggaggttctatagtgcttgttcttaatggatttt
atccagagggtgggtctt
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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