XB-CLONE-2105128
Clone Name: IMAGE:6879129
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Gene:
chrnb3.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC054966 [+]
GACGGATTCTTCTACTGTTGCTAGAAACTGCACAAATTGTCTCTCTGTATTTCAGGCTCATGATATTCTCAGTCACCGTAAACTGATCTATGGAAGCCAG
TCTCTGCAACTGATACCAAAAAGAAGTCTTCTTGAATATGATCCTCTCTGCTCATTTTCTCTCCTTTTCTATTTCGGTGTATTTCATCAACTCTGCTTTT
GCAAAGATTCATCCATTAGCAGAAATAGAAGATGCGCTCCTTCGAAAACTATTTGCTGGTTACCAGAAATGGGTCAGGCCTGTTCTAAACCCAAACAGCA
CCATTAAAGTTAATTTTGGTTTAAAAATATCACAGCTTGTTGATGTGGATGAGAAAAAACAGCTGATGACTACAAATGTATGGTTAAGGCAGGAGTGGAT
GGACTTGAAACTGAGGTGGAATCCTAAAGACTATGGGGGGATTACATACATTAGGGTTCCATCAGAAACTCTGTGGCTTCCTGATATTGTTCTATTTGAA
AATGCTGATGGGCGTTTTGAAGGTTCTCTCATGACAAAAGCAATAATAAAATATGATGGAACTGTAACATGGACTCCGCCAGCCAGTTATAAAAGTTCTT
GCACAATGGATGTGACTTTTTTCCCCTTTGACCGACAAAATTGTTCAATGAAGTTTGGATCATGGACATATGATGGGAACATGGTTGACCTTATTCTGCT
GGACGAGAATGTAGACCGGAAGGATTTCTTTGATAATGGAGAATGGGACATCCTTAATGCCACCGGCTTAAAAGGAATGAGGAAAGATGGGCTTTATTCT
TACCCATTTGTCACATATTCATTTGTTTTGCAACGTCTTCCATTGTTTTATACTCTTTTTTTAATAATCCCCTGCCTTGGTCTATCATTCCTAACTGTCC
TAGTCTTTTATTTGCCATCTGATGAAGGGGAAAAACTCTCCTTGTCCACCTCGGTATTGGTTTCTCTAACAGTTTTCTTACTAGTCATTGAAGAAATTAT
TCCTTCTTCATCAAAGGTAATTCCACTAATCGGAGAGTACCTTTTATTTATCATGATTTTTGTAATTTTGTCAATAATTGTTACTGTTTTCGTCATTAAT
GTTCATCATCGGTCCTCAGCAACTTATCATCCAATGGCACCGTGGGTAAAACGCCTTTTCCTGGAAAAGCTTCCGAAGCTTCTTTGTATGAAAGGACATG
TTGATTGCTTCTCCTTTTTTGATTCAGAAAACAATGTCTCTTCTGCCAAATCAAAACCCTCTGGCAAAAGGAAACAAATTAATGCAAGTGATGGGGAAAA
GGTCTTAATTGCCTTCTTGGAAAAAGCATCAGATTCTATTAAATACATTTCAACACATGTAAAGAAAGAGCATTTTATTAGACAGGTTGTTCAGGACTGG
AAATTTGTGGCTCAAGTTCTTGATCGGATCTTCCTATGGTTGTTCCTCTCAGTATCTATCACTGGATCAGTTCTTATATTTACCCCTGCATTACAGATGT
GGTTGAACAGTCATGTCTGAATCGTTGTTATAACAGCGACTATAGTAAAGGAGAATATAATAAACCTTTTTTATCATGTATATTAGACTGTGTTATGGTC
ATGGTGGGCTTTTTTGAAAAATAATCATCTATTCACAACAGGTTTTGTTTCTTAAGTCATAAATGATTTCACCATACTATCTATATGTGAGCAGAAATTT
ATAGTAAAGGTCCACAGTACACATATTTATATATTAGTGTCTGCAAACTGCTTTTCCGCACAATTGCTTAAGAACATTTTTACTTTACTAGGCCTCACTA
GACTTGAAAGATATGTTACAGAAGATTATTATTAATGCTGTTATGTATCAAACTTGCCTTTAAATTATATTCACTTGTCAATATGTCTATGTATTATATC
ATCATCTGTTAATTTTTTTTTTCCAATAAACTAAATTTGGTTGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB207069 [+]gggacggattcttctactgttgctagaaactgcacaaattgtctctctgtatttcaggctcatgatattctcagtcaccgtaaactgatctatggaagcc
agtctctgcaactgataccaaaaagaagtcttcttgaatatgatcctctctgctcattttctctccttttctatttcggtgtatttcatcaactctgctt
ttgcaaagattcatccattagcagaaatagaagatgcgctccttcgaaaactatttgctggttaccagaaatgggtcaggcctgttctaaacccaaacag
caccattaaagttaattttggtttaaaaatatcacagcttgttgatgtggatgagaaaaaacagctgatgactacaaatgtatggttaaggcaggagtgg
atggacttgaaactgaggtggaatcctaaagactatggggggattacatacattagggttccatcagaaactctgtggcttcctgatattgttctatttg
aaaatgctgatgggcgttttgaaggttctctcatgacaaaagcaataataaaatatgatggaactgtaacatggactccgccagccagttataaaagttc
ttgcacaatggatgtgacttttttcccctttgaccgacaaaattgttcaatgaagtttggatcatggacatatgatgggaacatggttgaccttattctg
ctggacgagaatgtanaccgggaaggatttcttttgataatggagaatgggacatccttaatgcccaccggctttaaaagaatgaagnaaagatgggctt
ta
3' EST: CB562523 [+]gtataatacatagacatattgacaagtgaatataatttaaaggcaagtttgatacataacagcattaataataatcttctgtaacatatctttcaagtct
agtgaggcctagtaaagtaaaaatgttcttaagcaattgtgcggaaaagcagtttgcagacactaatatataaatatgtgtactgtggacctttactata
aatttctgctcacatatagatagtatggtgaaatcatttatgacttaagaaacaaaacctgttgtgaatagatgattatttttcaaaaaagcccaccatg
accataacacagtctaatatacatgataaaaaaggtttattatattctcctttactatagtcgctgttataacaacgattcagacatgactgttcaacca
catctgtaatgcaggggtaaatataagaactgatccagtgatagatactgagaggaacaaccataggaagatccgatcaagaacttgagccacaaatttc
cagtcctgaacaacctgtctaataaaatgctctttctttacatgtgttgaaatgtatttaataaaatctgatgcttttttccaagaaagcaattaagaac
ctttttcccccatcacttgcattaaattttgttttccttttgcccgaagggtttttgattttggcaaaaaaagacattggtttttctgaaatccaaaaaa
ggaaaagccaatcaacatggtccttttcataccaaggaaaccttccggaagctttttcccgggaaaagggcgttttttacaccaccgggggccctttggg
gaagaaataagttttccctgaaggaaccccaatgtatttaaaacattttaatgggccccgaaaaacccggttaaccaaatttattttggagcacaaaatt
tttcccaaaaaattccttaggagaaan
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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