XB-CLONE-2105064
Clone Name: IMAGE:6877773
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Gene:
klf7.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC054963 [+]
GGGATCCAGGAGCAGGCAGCACTAGTACACAAATAGATGTCAGTGCATCTTTCTTTCTGGCACAAGCTCTGGAGTGACCACAGCCTTTGCAAAAGGATTT
CTTGCCTCCCATAACCACACTGGATGTTGACTTTTTTTTATTATTATTTCATTTTTTTCCCATTTCTGTCTCTTAAGCATCCTTCCTATTTTTCACATGC
TTGGGTTGGAGTGAGCAGCACTTCTATGATTTTTTCCCCCCTGTCATCTCCATTGACTGTAACAACCAACACAAAACAATTTAAAAAAAAAAAAAAAGTG
TGGGGGGGGACTGTGGATCTATTTTAAGAGGACAGCGATCAAGGAATATACATATTTTTTTTTTCTTGCATAGCAAACATGGATGTTTTGGCTAGTTATA
GTATATTCCAGGAGCTCCAGATTGTACATGACACGGGCTACTTCTCAGCAATGCCATCCCTGGAGGAAAATTGGCAGCAGACATGCCTGGAACTGGAGCG
GTATTTGCAGACAGAACCTAAAAAAATGTCAGAAACATTTGGAGAAGATTTGGACTGTTTTCTGAACACCTCCATTGCTCAGGCTGCTGATGGCACTGCT
TGCAAGATTGATTCAATTGTAATTCCAGTGGATATTAGTACCTATGAAAAACGTCCAAGCATGGATGTGTTACTTTCACAAGACAAGTTACTATCAGAGA
CGTGCGTCAGCCTACAGCCCGCTACAGAATGCTATACGTCTGTAAACCAAGCGCAGTTGAATGCAGTTACATCTCTAACGCCGCCATCATCCCCAGAACT
TAGCAGGCATCTAGTCAAAACCCCACAGTCCCTTTCTGTTGTAGATGGCACCGTGACCTTAAAACTAGTTGCAAAAAAGGCCACGGTTAGCTTGGGGAAG
GCTGCAGAGTCAGCATCAACGGCGGTGACGTCAAAGTGCGGCCAGAGTGATAGTGAACAGACTGGGGGCCCAGGAGAAGCTTCCCCTGAAAATAAGAAAA
GGGTTCATCGCTGCCAATTTAATGGCTGCAGGAAAGTCTACACAAAGAGTTCACATTTGAAAGCCCACCAGAGGACACACACAGGTGAGAAGCCATACAA
GTGCTCCTGGGAAGGGTGCGAGTGGCGTTCTGCTCGAAGTGATGAACTGACAAGGCACTACAGGAAGCACACAGGGGCAAAGCCATTCAAATGCAACCAC
TGCGACAGGTGTTTTTCAAGGTCTGATCACCTTGCCCTCCATATGAAGAGACATATTTAAGGACATTCAAAAATAGAGATGGCATCCCAGCCTTGAACAG
ACCATCTGTTGCAGTGGGCAATTGTGGTTATGCAGGAGAACATTGTCACATGTAAAACTCTGTACACAAAATCCATATCAAAAGTTCATGCACCGTCACA
CATTCATGCATACAACACCTGTTTGTTCTGCCTTACCCCCAGCCTAAGTGAGAAAGACATGGAAGACATGGATGGAGATTTGAATGATTGAAGATCTACA
AAATCCTAAGCAATTGCATTTTGGGTGATCTTGTATGTCTCACCATGGCAACTAAGAGAACAAGACATCTCTGCAAGTGGACAGTAGATTTGAGAGGCTC
TGAAGAACTCAATTTATTGCATCTCAGAAGCTGCTAAAATCCACTTTCGAATTGTAGCACTAAATATTTTGTTTTAAATCTTTGAGGCTAAAAAAAGATG
GATTTGCGAACTAGGAAACCAAATCTCAGTCCATGAGCTTTCTGCTTCATGTATGTGAATCTGAAGACTGCAAATCAAAAACAAAAAACTGTCCTATTTC
TATTAGGATCTAACTACATGTGGTTTTACCTGATTATGGAGCTTTTAAAACCCTTTTTAACTCAATGCCATAGGCCTTACTGGCAGTAAACAGGTGAAGT
TCCAAGCATGACCAAGGTTCTGCAATTGTTTGGTCTATGTTTCATACAGAACTCTTCAATGGTAAAACATTTATATCCCCTCATTCCCTTAATTTATTTG
TTTTTTACTTATTTTGGTGTGGCACACCTTATGCACTAGTGACTTTTCAAGCCGCTAACACTGACTGAGTGGGATCAAAATGATTTAGCTAGAGTGTCCT
ACCAGTGACTACCAGTGTGCACACTTCCAAAGGTTACAGACCTTACAGTTTTTGTTTTGTCTTTTTTTAACTTGTTTTTAATTTAAACAGGCTCAATGAG
TAACATTGTTCAGTTACAGTACATTCTCTCGTTGAACATTTATCTTATTCAACTTCTTGCTCTCTGTCTGGACACTTTTAGCTTGGATTGGACCAGCAAT
TTAGACTTGACTTCTAATGTCAGTGGGCTGTAAGCCATCACCAACTGAACTTTCCAAAATCGTGCTTGATTAGTGATTTTTAATCCTAACAATATAACTA
GTTAGAAACTTGATGTTATTTCGAGAGCAGCTGCATTTTTGTACTAAATATTTTGTTTGTGTCTAACAACAAAAAAGCCAAATATATAAATCCTTACAAC
ACTGGATATTGGAGGACACCTTAAAGTACAGTAAGTTGATTTTGTGGGATTGACTCATTAATAAACGAAAGAGGAGACACTACCTTGAATACCAAGGTTA
TTATTTAGGATGCTCTTAAATTAGTTAAGAGCTCAGATATCTGTAAACTAAGTGGATATAATTTCCATTGGTGATTTTTTTACAGGGTTACAGTAATATG
TTAGAGCTGTAAATTCATTGTAATCTCATGTTAGTAATATCTTCGGGTCACAAAACAGCTTTATATACCCAACAAAATCAATCATTTTCTCATATATTTA
AAACGGCCCTGAATCCATCATGATCAGGAAATTTGTGTACATACTACTATGCTGTCACTAGTCTTGCTTCTAGAGACTGCAAATTGTAACAAGTGGTCAA
TTCAAGTAGTTGTGGAGGGGGATAGGTTATCTATTTTGGATATTCTGGGGCCATCCCTAAACAACTAGTAAAATTGCACATATGGTCCCATGGTGTCCCT
GACAAAAGCAGCTCTTTTATCCCAGCCAACTGGCATATTTTTAACTTGTTTTGACTCCTTGACTGTTAAACGGAAAACAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAA
5' EST: CB206449 [+]ggagcaggcagcactagtacacaaatagatgtcagtgcatctttctttctggcacaagctctggagtgaccacagcctttgcaaaaggatttcttgcctc
ccataaccacactggatgttgacttttttttattattatttcatttttttcccatttctgtctcttaagcatccttcctatttttcacatgcttgggttg
gagtgagcagcacttctatgattttttcccccctgtcatctccattgactgtaacaaccaacacaaaacaatttaaaaaaaaaaaaaaagtgtggggggg
gactgtggatctattttaagaggacagcgatcaaggaatatacatattttttttttcttgcatagcaaacatggatgttttggctagttatagtatattc
caggagctccagattgtacatgacacgggctacttctcagcaatgccatccctggaggaaaattggcagcagacatgcctggaactggagcggtatttgc
agacagaacctaaaaaaatgtcagaaacatttggagaagatttggactgttttctgaacacctccattgctcaggctgctgatggcactgcttgcaagat
tgattcaattgtaattccagtggatattagtacctatgaaaaacgtccaagcatggatgtgttactttcacaagacaagtactatcaggacgtgcgtcag
cctacagccgctacagatgctatacgtctgtaacaggcantatgtatctagactaagnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnna
3' EST: CB562555 [+]aaaaatattgccangttggctgggataaaagagctgcttttgtcagggacaccatgggaccatatgtgcaattttactagttgtttagggatggccccag
aatatccaaaatagataacctatccccctccacaactacttgaattgaccacttgttacaatttgcagtctctagaagcaagactagtgacagcatagta
gtatgtacacaaatttcctgatcatgatggattcagggccgttttaaatatatgagaaaatgattgattttgttgggtatataaagctgttttgtgaccc
gaagatattactaacatgagattacaatgaatttacagctctaacatattactgtaaccctgtaaaaaaatcaccaatggaaattatatccacttagttt
acagatatctgagctcttaactaatttaagagcatcctaaataataaccttggtattcaaggtagtgtctcctctttcgtttattaatgagtcaatccca
caaaatcaacttactgtactttaaggtgtcctccaatatccagtgttgtaaggatttatatatttggcttttttgttgttagacacaaacaaaatattta
gtacaaaaatgcagctgctctcgaaataacatcaagtttctaactagttatattgttaggattaaaaatcactaatcaagcacgattttggaaagttcag
ttggtgatggcttacagcccactgacatttagagtcaagtctaaaatgctggtccaatccaagctaaaagtgtcccgacggagagcaagaaagttgaata
agataaaatgttcaacgagagaatgtactgtaacctgaacatgtttactcatttgaccctgttaaatttaaaaacaagttta
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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