XB-CLONE-2098726
Clone Name: IMAGE:6874241
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Gene:
eno1.S
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC054169 [+]
GTTCTCCCCAGTCATTGTCGGAAGAGAACGGTTGCACCCACAAGAAAGTGAAAATGTCTATCAAGAACATCCGAGCACGTGAAATATTTGATTCTCGTGG
GAACCCTACAGTGGAGGTTGACCTATACACTTGCAAAGGACTGTTCCGTGCTGCTGTTCCAAGTGGAGCTTCCACTGGTATTTATGAAGCACTAGAACTC
CGGGACAATGACAAGACTCGCTACCTGGGAAAGGGTGTGTCTAAAGCAGTTGAGCACATCAATAAAACCATTGCACCTGCATTGGTAGGCAAGAATCTAA
ATGTTGTTGAGCAGGAAAAGTTTGACAAGCTCATGATTGAAATGGATGGAACTGAAAACAAATCCAAATTTGGTGCTAATGCACTCCTGGGAGTGTCCCT
GGCAGTGTGTAAAGCTGGAGCTGCAGAGAAGGGAGTCCCACTGTACCGTCACATTGCTGACCTGGCTGGGAACCCTGAGGTCATACTTCCTGTACCGGCA
TTTAATGTTATCAATGGTGGCTCTCATGCTGGCAACAAGCTGGCTATGCAAGAATTCATGATCTTGCCTGTGGGTGCAGACAGCTTTAAGGAGGCCATGC
GCATAGGGGCAGAGGTGTATCACAATCTGAAGAATGTCATCAAGGAGAAATATGGAAAGGATGCCACAAATGTTGGTGATGAAGGGGGTTTCGCTCCCAA
TATCTTGGAGAACAAAGAAGCCTTGGAGCTTCTGAAAACTGCCATTAACAAGGCAGGATACCCAGATAAGATTGTCATTGGGATGGACGTGGCAGCATCT
GAATTCTACCGTGATGGAAAATATGATCTGGACTTCAAGTCCCCCGATGACCCCAGCAGATACATCTCACCTGACAAGCTGGCTGAACTGTACATGAGCT
TTGTCAAAAATTATCCAGTGGTATCCATTGAAGATCCCTTTGACCAGGATCATTGGGAAGCCTGGACAAAGTTCACAGCAGCTTCTGGTATCCAGGTAGT
TGGTGATGACTTGACTGTGACCAACCCTAAGCGTATTGCCAAGGCTGTGGAGGAGAAGGCTTGTAACTGTCTGCTTCTGAAAGTCAATCAGATTGGCACA
GTGACAGAATCCCTGGAGGCGTGCAAGCTGGCTCAGTCAAATGGCTGGGGTGTGATGGTCAGCCATCGTTCTGGGGAGACAGAAGATACTTTCATTGCTG
ACTTAGTGGTTGGTCTTTGCACTGGACAGATAAAGACTGGGGCTCCATGCAGATCTGAGCGACTTGCAAAGTACAACCAATTGCTCAGGATTGAAGAAGA
ACTTGGCAGCAAGGCCCGCTTTGCTGGAAAAAACTTCAGAAAACCAGTTTTCAACTAATTGGCGACTTCCCTAGGCAATCGCCCAACTGATGTAGTAGCT
GTGCACCTAGGAGAGACTACAAGAGGTGGCTTACTTGTCACTGCTGAGCCCTCGCTTAATTTCAGGCACTTCCAGCGATGTTAGATGTTACGGAGCCTCT
CCCAGCATAACCCTGCCATCTTTCCAATTCAAAAGCTTTGCTGCTGCTGTTTAGGTTTACTCCTAATTCAGTGTCACTCTGGTGCCTGCATGTCGTACTT
GTTTTTTCTATTGTGGCTGTGGTCCTGGTCACGTCTTCTTTGTAGTCCTCTGTTCTCATCCTGTTGCAGTTTCACAATAGATAATGGTTTGTTACACTGT
AGAGATGAAGAGGCAATAAAACTCTTTTGAAGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAA
AAAAA
5' EST: CB199223 [+]cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggttctccccagtc
attgtcggaagagaacggttgcacccacaagaaagtgaaaatgtctatcaagaacatccgagcacgtgaaatatttgattctcgtgggaaccctacagtg
gaggttgacctatacacttgcaaaggactgttccgtgctgctgttccaagtggagcttccactggtatttatgaagcactagaactccgggacaatgaca
agactcgctacctgggaaagggtgtgtctaaagcagttgagcacatcaataaaaccattgcacctgcattggtaggcaagaatctaaatgttgttgagca
ggaaaagtttgacaagctcatgattgaaatggatggaactgaaaacaaatccaaatttggtgctaatgcactcctgggagtgtccctggcagtgtgtaaa
gctggagctgcagagaagggagtcccactgtaccgtcacattgctgacctggctgggaaccctgaggtcatacttcctgtaccggcatttaatgttatca
atggtggctctcatgctggcaacaagctggctatgcaagaattcatgatcttgcctgtgggtgcaaacagctttaaggaggccatgcgcataggggcaga
ggtgtatcacaatctgaagaatgtcatccaggagaaatatggaaaggatgccccaaatgttgggtgatgaagggggtttcgcctcccaatatcttgggag
aacaaagaagccttggagccttctgaaaactgcccttaaccagggaggataccccagataaagatg
3' EST: CB565724 [+]gttatctattgtgaaactgcaacaggatgagaacagaggactacaaagaagacgtgaccaggaccacagccacaatagaaaaaacaagtacgacatgcag
gcaccagagtgacactgaattaggagtaaacctaaacagcagcagcaaagcttttgaattggaaagatggcagggttatgctgggagaggctccgtaaca
tctaacatcgctggaagtgcctgaaattaagcgagggctcagcagtgacaagtaagccacctcttgtagtctctcctaggtgcacagctactacatcagt
tgggcgattgcctagggaagtcgccaattagttgaaaactggttttctgaagttttttccagcaaagcgggccttgctgccaagttcttcttcaatcctg
agcaattggttgtactttgcaagtcgctcagatctgcatggagccccagtctttatctgtccagtgcaaagaccaaccactaagtcagcaatgaaagtat
cttctgtctccccagaacgatggctgaccatcacaccccagccatttgactgagccagcttgcacgcctccagggattctgtcactgtgccaatctgatt
gactttcagaagcagacagttacaagccttctcctccacagccttggcaatacgcttagggttggtcacagtcaagtcatcaccaactacctggatacca
gaagctgctgtgaactttgtccaggcttcccaatgatcctggtcaaagggatctttcatggaaaccacttggaaaatttttggacaaaacttctgttacg
gttcaaccccgcttggccagggggaaaatgtatcctgcctggt
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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