XB-CLONE-2094982
Clone Name: IMAGE:6934504
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Gene:
ighv4-31.L
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Species: Xenopus laevis
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Sequence:
Full length sequence: BC081242 [+]
GGGGACTTCATCCAAACACATCTTCTGCTAACATGGAAAGCGTTTTTCTTTTAATTTTCTTGGTGTTTGCCCCATTATGTGTCCTCTCTCAGACATTACA
AGAGTCTGGTCCTGGAACTGTGAAACCCTCAGAGTCACTCAGACTGACCTGCACAGTTTCTGGGTTGAGCTAAGCAGGTATTATATTAGCTGGGCCAGGC
AGCCTCCTGGGAAGAGACTGGAATGGATTGGGCTTGCATATGCTGATGGGAGCACAGCTATTGCTGATTCACTAAAAAACAGAGTTACAATTACTAAAGA
CAATGGGAAGAAGCAGGTCTACCTCCAAATGAATGGGATGGAAGTTAAAGACACTGCCATGTATTACTGTGCAAGAGGTAGCGGGTACAACTTTGACTAC
TGGGGACAAGGGACCATGGTCACCGTCACTTCAGCTACTTCAAAATCCCCATCCCTTTTTCCACTCATTTCTTGTGGGGAGTCTATGGACCCAGTCACCA
TTGGTTGTTTGGCCAAAGATTTTCTCCCTGAAACTATTAGCTTCACCTGGGGAGATAAGAACAATGCCAGTTACTCCACTGGCCTCAAGAGCTATAAACC
TGTGATGCAATCTTCCGGCACCTACTCAGCCAGCTCCCAAGTCAACGTTGCCTCTGCAGTCTGGGACAACATTGAACAATTTTACTGCAATGCCAAACAC
CTGGATACCATTAAGAGTGTGGAACTCAAGAAAGATCCAGTGAAACCAGTTGAAAAACCAGTTGTGTCTATTCACCCTCCATCCAAGGATGCTCTTGCCT
TGAATGAAAGCCTCTTTATCGTATGTCTTGCAACGAATTTTACTCCCACACATATAGTTATTAAATGGTTAAAGAATGGGAACCAGACAACAGAAGGTGT
CAGAGTTGAAGAACCTGTTGAAGACAAAAAGCGAGGATATGAGGCAACATCCTATCTCTCCATAACTAGAAAGGAATGGGATTTGGATACTTTGTACTCA
TGTGTAGTTGAACATGCAGAATCGGGTTCCTTACAAGAGAAGAATATGAGCAAATCACTAATGTGCGACACCCCAATAACACCCACTAGTATCCAAGTAA
TCACTATTCCACCATCATTAGAGAGCATTTTTGAGAAAAGATCTGCCACACTTACCTGCCTGGTCAGTAATATGGCTAACTCTGAGGATTTGAGATCAAT
ATCCTGGTTTAAAAAATCTGGTACTCAAGAGATACCATTGAAAACAGAACTGGGAGATGCAATCTATAATGATAACCGCACCTATTCTGTAAAAGGAACC
ACCACTGTCTGCGCTGATGAATGGAATAACGACAAGTTTGTCTGCAAAGTGGAACACACAGAGCTGGCTTCAATGAAGGAGGTCTTTCTCTTTAAAGAAA
AAGGAGAATACAATACACCATCTGTATATGTTTTCCCACCACCTCTTGAGGAATTGTCTAAGAGAGAAACTGCCACCTTGACATGCTTGGTTAAAGGGTT
CAGCCCCTCTGAAATATTTGTAAAATGGCTTCACAAAAATGAGGCGGTTCCAAAACAAAATTACATAAATACCAGCATCAATGACGAGCTTTTACCCAAA
GGACAGAAGAGTGGAAAGTTCTTTCTGTACAGTCTTCACACCATAGACATTAAAGACTGGGATGCTGGTGATAGTTTTTCCTGTGTGGTTGGCCATGAGT
CATTGCCACTTCAGCTGACCCAAAGGAGCATTGACAAGTCTTCTGGTAAACCTACTAACGTGAATGTGTCCCTCGTCTTGTCTGATACCTGTTAGTGATC
ATCTCCAAAGACCCTATAACTCCATCTCATGTTCTTCCCTATATGGTGTAATGGACAAGGCGGAGGGAGAAGTGTTGACTTTATGTTTGTCTGTCTGTTC
TTGTACATTTTTTGTCTGCAAAATTAAAAGTAGAAATAAAAATCAATTTATACAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAA
5' EST: CB942767 [+]gggacttcatccaaacacatcttctgctaacatggaaagcgtttttcttttaattttcttggtgtttgccccattatgtgtcctctctcagacattacaa
gagtctggtcctggaactgtgaaaccctcagagtcactcagactgacctgcacagtttctgggttgagctaagcaggtattatattagctgggccaggca
gcctcctgggaagagactggaatggattgggcttgcatctgctgatgggagcacagctattgctgattcactaaaaaacagagttacaattactaaagac
aatgggaagaagcaggtctacctccaaatgaatgggatggaagttaaagacactgccatgtattactgtgcaagaggtagcgggtccaactttgactact
ggggacaagggaccatggtcaccctcacttccgcttacttcataatccccttccctttttccactcatttcttgtgggggagtctatgggacccagtcac
catttggtttgtttgggccaaaagatttttctccccctgaaaactaattagcctttcaccctcggcggaagaataaaaaaccaatggcccagtttaactc
ccactgggcccttcaaagaaacctaataagaacctggtgtatcggcaattctttccggggcaccaaactcaagcccaaccttcccaaaattcaacagttg
tcgcctactgcccatcaggggaaacaaaaatttggaaacatattttaactgggcaatggcccaaaacaacccggggatataccaattaaaaaagggggtg
gacaacctccacagaacaaaaatccaccttgtcaataccggttttgaaaaaaaccaccgtcggtgtgttcattttccccacctcccatttcacgctggag
agtgccctttgccccttttagaaggaaaacc
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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