XB-CLONE-2080666
Clone Name: IMAGE:6877311
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Gene:
lmo4.2.S
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Species: Xenopus laevis
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Sequence:
5' EST: CB206132 [+]
aatcactcctatagggcgctagctcgcctcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacatta
tacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggatgcatccatttatataatgatccatcagggcctggggg
atggcagcaggtaggaaacccacccttggccaatgtgcggattgaggctactgttggatttatactgactgtgcatgctgggagatgaagttcatcagac
atttcatggctgagcatgatattggtgtggtccctttctgttatgctggtgcaggagcctgatattgtgtaattaacataattaacggagtcgctgattt
tctgttcccaccccctgtgactgatgggagttgtagttaaggaacaaccgttggatctcctggctttcagttcagtacgaccagtgtagttcccccatgg
agggctaacatcagggtgatccaggccacagccctagtgcagttgctgaactacagacaaggagagagtgggcacagatctgtaagtggtttgggggtgc
agtagccagtcattttatgtctggtctcagaacagcacttaatttggggggtgagcttcgggtggaaaagcactggattgtcacgggcgctcaattctaa
atccagcctgggactaatgagggacattccaaccccccaccaaaaacattatgtctggagtccaatatcccacatacgtcccctccctgagaatgtgggc
ccagtgcgcccagcttgggggccacggcctcttcctgacccaccaatggcacaaggaacactggcaccctcttttctaaccggctgaaatttaaaccctg
gcaaacaatgcttgggtaagtggaatgccgggggaaggaactcggccccggggtgaacaacccccccccttataaccctgac
3' EST: CB756353 [+]catgttttttttttttttttttttttttttttttgcatggaaagttgaatttttattcttaataacagcatcaaaatagggtgtagggaagaaaaaagtg
caaagttgttttttttgttacaaaaccggtttccgcccctaactttggacaaaacacacaacagaaagcacaaaattcctttattccaattatttacaca
ggatgttttatagcggaaagggatgggggggggggctggccccactaggggtgagcaagaagtatatcccccaaaactagcagtatccttcaagaacaaa
atactgtcttgaatactgtgtgcaggtggggccccctaggcactgactcacacctcactgatcccatcagccagatgacaaactaatatgcagctggggg
cacagggcacatttaaaataaaatttcatccccaatatgtgttctgtgggtctgatgtctggttattattggggggctgctcaccctgtgccagatcctc
ccctgcattcacttaccaaactatttgccatggttaaaatcagcaggtagaaaaaagggtgcagtgttcctgtgccattggttggtcaggaagaaccgtg
gcccccaactggggcactgggccacattctcagggagggacgtatgtgggatatttgactccagacataatgtttttggtgggggggtgggaaagtccct
cattagtcccaggctggaattagaaatgagcgccccgtgaaaatccagtggcttttcccccccaagccaccccccaaattaagtgggtgtttccgagacc
cggacataaaaatggctgggctactggccc
3' EST: CB755840 [+]tcaaataagggtggtagggaagaaaaaagtgcaaagttgttttttttgttaaaaaccggtttccgcccctaactttggacaaaacacacaacagaaagca
caaaattcctttattccaattatttacacaggatgttttatagcggaaagggatgggggggggggctggccccactaggggtgagcaagaagtatatccc
ccaaaactagcagtatccttcaagagcagagtactgtcttgaatactgtgtgcaggtggggccccctaggcactgactcacacctcactgatcccatcag
ccagatgacaaactaatatgcagctgggggcacagggcacatttaaaatagaatttcatccccaatatgtgttctgtgggtctgatgtctggttattatt
gtggggctgctcaccctgtgccagatcctcccctgcattcacttaccaaactatttgccatggttaaaatcagcaggtagaaaagagggtgcagtgttcc
tgtgccattggttggtcaggaagagccgtggcccccagctggcgcactgggccacattctcagggagggacgtatgtgggatatttgactccagacataa
tgtttttggtggggggttggaatgtccctcattagtcccaggctggatttagaattgagcccccgtgacaatcccttgcttttccaccccgaaacctccc
ccccaaattaaatgcctgttctgagaccagacctaaaaatgactggctactgcaccccccaaaccactttacagaatcttggccccacttctcccccttg
gccggaaaattcaagcaaactggcactaagggcttgtgggccttgaaattaccccctgtatgccttgcaccctccctg
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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