XB-CLONE-2080540
Clone Name: IMAGE:6877073
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Gene:
spcs2.S
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Species: Xenopus laevis
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Sequence:
5' EST: CB205914 [+]
gactcctatagggcgctagctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacattatac
gaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggattcgcgggatgagagaagatggcggcgagaggcggaaaga
acgggctgctggagaaagtaagcgagtggaagattgatgataaaccagtaaagattgacaaatgggacggatctgccggtgaaaactccctggatgatgc
agccaaaaaggtctactggagaattaagaatttggggaaaactttcggctgattggcgggccaattctttatttgggcccctcccctggggttttgtttt
ttgggggccccccggggggggaattttttgcccccctttcccccaaaattaaaaccggtttgggggcccttctgggggaaatacccaattttcccggggg
ggggggaaaacccggggggggtttaaaccccccccaataaaaggggaaaaaaaaatttttccgggggggggccccccaaaagaagagccccccccccggg
gggggggcccccccccacaaaatttttggggcccccccccccccccccccccccccaaggggtggttggttgtgaaaaaaaacccccccccggggggggg
ggggggtgcttccctctccccaccggaaaaaaaacaacacccccccctcctttcggggtgggggggggggggggttctcccccccccccccccctggtgg
gggggggggtgtgtttttttttttataataaaaaacagccgcccccgcccccggggaggggggggtttttttttttataaaaaaaaaaaaaaaacccccc
cccccccccacccataaaacatatgctttcgggcgcggcggaggaaaaaaaaaaaaaaaaaaaaantgttatttttttgttggggcccccccccctcccc
ccttttttgttgttttttgctggcgggcaccgcgcccccc
3' EST: CB756416 [+]catgttttttttttttttttttttttttttttttaaaaaaattctcctttattttttattggcctaaagagaacgcggagcttttacacaagtttcaatg
cgtttacatttcttctctcagacagtgaacagaaacaatggaaggattttattccaccaaataaatataaaaaataaagaatggacagatttctgcaaat
ctaaggttttgtcctgagatccttaaaaacatatattttaactggaaaaagggtatagttaaaggataagtaaacctctgaaacaagtgaatgcaaaatt
gacgagggggcccctccaagcacccccgcaatatacatccatcacccattttgcctttattccaaaatattaaaggatacatgtactgttaacatgaatg
aattttgtcacaacagcgccacctgctgggtagtttcccaccagttgtaacacaattcattcatatcaacagcacatgcatcccccaatatctcggaatt
aaaaccaataatgaatgtacactgccaaagggcccagaaaagcccccccgtccttttacactcgcctattttaaaggattacccaccctttaatcgcctt
cccccgcctaaaaaggagagatttttttgggcaaattaggtgacagtttatgggccgcaaatgcccttgggtaaaaaaaacaaggggggttttatcccca
tcccctggggttatcgggccattgaaaaatgtatttttttttaacccaaaccgggggttgttggccaattgaaagaaaaatttcctccggcgggggaaac
cccaaaacgtccaccccaaaatggatccccccaaatcttcatgggggaatatgtttgtgggccccccctctttctccgcacaaaaaa
3' EST: CB755886 [+]tacaacgcggagcttttaccgagtttcatgcgttacatttcttctctcagacagtgaacagaaacaatggaaggattttattccaccaaataaatataaa
aaataaagaatggacagatttctgcaaatctaaggttttgtcctgagatccttaaaaacatatattttaactggaaaaagggtatagttaaaggataagt
aaacctctgaaacaagtgaatgcaaaattgacgagggggcccctccaagcacccccgcaatatacatccatcacccattttgcctttattccaaaatatt
aaaggatacatgtactgttaacatgaatgaattctgtcacaacagcgccacctgctggtcagtttcccaccagttgtaacacaattcattcatatcaaca
gcacatgcatcccccaatatctcggaattaaaacaaataatgaatgtacactgcaaaagtgcccagaaaagcccccccgtccttcacactcacctatttt
aaaggtttacctatcctttaatcgtcttcccagctaaatagagagatttttgtgcaattatgtgacagtaatggccgaagtgccttgtgtaagaatcatg
tggttctatcccatacactgggttatcggccatgacataagtatttttatagccaatcggtgctgttgcaattgatgaccattctctgcatgagacccaa
aaagcacacaaatgattcgcgcagttccaggggaatgtgtgtgggccccctttctgcacataattcctgcccactaatgacaaagtggcccgttgggaaa
aaactatttccgataagggggtatttccaggaaaaaaacccttaactgtgggcccttt
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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