XB-CLONE-2080354
Clone Name: IMAGE:6877279
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Gene:
itsn1.L
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Species: Xenopus laevis
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Sequence:
5' EST: CB206102 [+]
tcctatagggcgctagctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacattatacgaa
gttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggggaatctttcagatattgaccaagatggaaaactaacagctgaag
aatttatattagctatgcacttaatagatgtggccatgtctggccagccacttcctccagttctgcctcctgattatattcccccaacttttagaagagt
tcggtcagggagtggcttatccgttattagcgcagtgtctgttgatcagcgattaccagaggaaccggcggaagaggagccacaaaatgcagatagaaag
ctcccagtgacatttgaagacaagaaaagggagaactttgagcgaggaaacctagaattagaaaagaggagacaggcacttttagagcagcagcgcaaag
agcaggagaggttggctcagctggaaagggcagagcaggagagaaaagaacgagagagacaagatcaagagcggaagcggcagcaagaactggaaaaaca
gctggagaaacagagagagctggagcggcaacgggaagacgagcgcagaaaggaaattgaaaggagagaggcagcaaaaagagaacttgaaaggcagagg
cagttggagtgggaacganacaggaggcaagaactctttaatcaaaggaacagagagcaggaagacattgttgtcttanaagcaaagaagaagactctgg
agtttgagctggaagctctanatgataaaaagcaccagttggaaggaaaactcccaggatatccccgctgtcgtctcactacttcacgacacggaaatag
gaaagcnactaacaaaatcccaggggaactggaggnatcgc
3' EST: CB756509 [+]catgttttttttttttttttttttttttttttttcggatgaggataagttggccaggagccagcgttagctgctcaggaccagttgctgtataagatgca
ataacctgggcgatttctggtttctttccaagacttcctgtctttcctgcgcttccagcagcctctgagtctttgggcctcacatagttggaaggaaaaa
ctcccgtcttatctcccacagtgccagtccaccagtcgccatcttttttaatcaccacaatcagatctccttgctgaaacgtcaagtctccctgctcgtt
gctctcgtatgtgtacatggctatgaattcttctccttgggttgcaggtttaaatgctggtgaggagactctctttaaactcgccggactttctgaagat
gtggaatcaatgcttgtagatttccttagtggaccggctataagctttacataggatttgggaaaccagcccttttgaccttgaacttccccaaaccacc
acatatcctgctgttcaagaaccgtgataatatcatttttgttaaaattgagatggttgtccttctttgctctccacggatacaaggcttgtgcctgaag
accttctactttttcaccctgaccaagtacaggagatggcgatgatccagtaacagttgctggggtaaaagcaaagcgctgcctgtgctgaccagcacta
ggaacagtgagcgatggttgaagctgccccacgttgtccccagttaatcacctcctcctactttttatcccgttgttttatttggtttggggcccacccc
3' EST: CB756047 [+]gctcaggaccagttgctgtataagatgcaataacctgggcgatttctggtttctttccaagacttcctgtctttcctgcgcttccagcagcctctgagtc
tttgggcctcacatagttggaaggaaaaactcccgtcttatctcccacagtgccagtccaccagtcgccatcttttttaatcaccacaatcagatctcct
tgctgaaacgtcaagtctccctgctcgttgctctcgtatgtgtacatggctatgaattcttctccttgggttgcaggtttaaatgctggtgaggagactc
tctttaaactcgccggactttctgaagatgtggaatcaatgcttgtagatttccttagtggaccggctataagctttacataggatttgggaaaccagcc
cttttgaccttgaacttccccaaaccaccacatatcctgctgttcaagaaccgtgataatatcatttttgttaaaattgagatggttgtccttctttgct
ctccacggatacaaggcttgtgcctgaagaccttctactttttcaccctgaccaagtacaggagatggcgatgatccagtaacagttgctggggtaaaag
cagagcgctgcctgtgctgaccagcactaggaacagtgagcgatggttgagctgcccacgtgtcccagttatcactctctactttatccgtgttattggt
tggccacgttgagctgaagtctgcccagttgttggaatttgcagatgtatctgtagaagctgcagtggaaggtgccaaatggacagtgggtttggctgta
atgtctgctgtttgtttagttgtgtctgggaaattcactctcaggca
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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