XB-CLONE-2080188
Clone Name: IMAGE:6877160
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Gene:
ckm.L
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Species: Xenopus laevis
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Sequence:
5' EST: CB205995 [+]
gactcctatagggcgctagctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggccgcataacttcgtatagcatacattatac
gaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggggggtcaccctgaagtttgctccgtctctacagcaggtctag
cccgatcttccaacatgcctttcggtaacacccataacaactttaagctgaattattcagctgaggatgaatatcctgacctcaccaagcacaacaatca
tatggccaaggtcctgactcctgacatctacaagaagctcagggacaagcagacaccaagtggattcaccttggatgatgtcattcagactggagttgac
aacccaggtcaccctttcatcatgactgtgggctgcgtaccaggagatgaggaatcctatgaagtgttcaaggaccttttggatcccgtcattgaggatc
gtcacggtggttacaaaccaactgacaaacacaagacagacctcaacttttgcaaccttaagggaggtgatgaccttgaccccaactatgtcttgaacac
ccgtgtaaagactgggccgtatcattaagggatataccctgcccccccactgcagccgggggggaacgtcgtgctgttaaaaaattctccattcaacctc
tcaacagccttaggggggaaattcgggggaaaaaactccccctcccaaggatatgcccaatggcaaaacancaaccgtttgattggaccccccctccctg
tttggaaaaaaaaagggcccccttttttttttggccccttggatgggccccaaaattggcccttacgcccgcgtgttatctggaacaaaaaaaaaaaaaa
acttttcggggggggggggaaaagaaagaaggaccccttggaaaatcttttttctttcccnaaaagggggggaaactttaaaaaactt
3' EST: CB756592 [+]catgtttttttttttttttttttttttttttttttagaggtgatattttggttttattgtctccaggcaatcatgggggtggtttcacaaaacagatggt
ggaaggagggtaaatgtgttataaatagaaccaactctgtctctgcaataatataatctttaacagagaatgagattcatcttctcttttacgttgattt
tgaagctgtgtcttttcttgtggtggaacctcctaataatctgacacatctgggattattccacccacctccattactaatgtattcccccctgtagatg
gccattttccgacctctaatgatcttaatggggtctcctcttctctttgatggtggtaagggttagggtgtttaggctctgtctctagcccatcatctac
ttctgtgctggcatcatatcatcaatggtctgtcccttctcaagcttcttctccatctcaatcatgagtttcacaccatctacaaccatctggacctgat
ccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtcaacaccacctgtgcctctcttctgcagacgaag
cctggtcagaatttcctcaaatttggggtgtttgctgaagttggggaacttggcatggacacctcctctcaaacctgttcccaaatttgatggggcagta
aagacctaaacccaaatgctcgttccacaatgaaaggggtgtccctgccttgttttaaaaaattctctttcaatctttttgtaaattccccctccccaaa
aaaccgtccctga
3' EST: CB755942 [+]tcaaaaagggggtggtttcacaaaactgatggtggaaggagggtaaatgtgttataaatagaaccaactctgtctctgcaataatataatctttaacaga
gaatgagattcatcttctcttttacgttgattttgaagctgtgtcttttcttgtggtggaacctcctaataatctgacacatctgggattattccaccca
cctccattactaatgtattcccccctgtagatggccattttccgacctctaatgatcttaatggggtctcctcttctctttgatggtggtaagggttagg
gtgtttaggctctgtctctagcccatcatctacttctgtgctggcatcatatcatcaatggtctgtcccttctcaagcttcttctccatctcaatcatga
gtttcacaccatctacaaccatctggacctgatccacttcagagaagcccagcctatcagcattggagatatcaaagacacctcctactgctgcagtgtc
aacaccacctgtgcctctcttctgcagacgaagcctggtcagaatttcctcaaatttggggtgtttgctgaggttggggagcttggcatggacacctcct
ctcagacctgttcccaagtttgatgggcaggtaaggacgtaacccagatgctcgttccacatgaaggggtgtcctgcttgtttaaagatctcttcaatct
ttgtaagtccctcgcagaagcgtctgaagacttctttcatgtttcctcctttctgcatggaaatgactctcaaatggtcctcttcatttacccacaccag
gaaggtcttgtttgtccttgtgcccgataat
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.6 kb (range 0.9-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library.Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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