XB-CLONE-2079882
Clone Name: IMAGE:6873679
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Gene:
ola1.L
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Species: Xenopus laevis
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Sequence:
5' EST: CB198686 [+]
cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggggagtgagagatc
ctgaggccccacgcctgcattcttggaagccttcctatttcgcctgcaagtacacgtgctcagcacctaggatgccacccaaaaaggctgacgatggacc
aaaacagcaccctatcattggaaggtttggaacctctttgaagattggaattgttggactgccaaatatagggaaatcaactttctttaatgtgttgact
aagagccaggcagcagcagaaaactttccattctgtaccattaatccgaacgagagcagagtccctgttccagatgacagatttgaattcctgtgtgagc
accacaaacctgcaagcaaagttccagcctttctgaacgtggtggatatagcaggcctcgtgaaaggagcaagtgcaggacaaggcttgggcaatgcatt
cttgtcacacattagtgcgtgtgatggaattttccaccttatgcgtgcttttgatgatgatgatatcatccatgttgaaggaaacgtcaaccctgtgcga
gacattgagatcatacgcgaagagctccggctaaaggatgaagaaatgattatagctgctcttgataagcttgagaaagtggcagtaagagggggagaca
aaaagttaaaaccagaatatgacataatggtgcaaaagtcaaaaacctgggttaatagaatgaaaaagaacccatggtgcg
3' EST: CB756745 [+]catgttttttttttttttttttttttttttttttatcaaatgttttattgagtgtcgacatctggagtactgtaaaacatgcattatctgtagattccga
acggagcgccgcacattgtcctttctgtcagatgtgtcagccttggcatacatgatggagattaatgaactatcatgagacttgggtggttgcagagagg
cttctgtgctttgcagaagggtcttaatcatctgaaaagcacattgactgagctgctattgcttgcaggaggtgacattccaactgcaatataaacattt
tgttttgttttccttgggggggggggatcatttcactgtcttgttattcttttaagcagattattctgtctaacttaaaaaaaaaaataataattttacc
aaagaagtagaggattttttttctcctacttctgattgaaagttaaaaattatttcttttttgattgctgaggtgtattaaatttaaagaagataatgtc
tccgtcttctactgtgtaatttctgccttgttgtctgtattttcctgcagccttgacagatgcctctgtgccttcttctttgaaatcgtcaaatttcatt
acttccgccataataaaacccttctcgaaatctgtgtggatcttgcctgcagcctgaggagcctttgtccctttcttgatagtccaggcacgtacttcat
cggggcccggggtgaagaagtactccagttggagtgctgcatatcccggttttgataatccttggataagaacactttgggggcattttccccttccaga
aaactttctggcttc
3' EST: CB756100 [+]gattatctgtagattccgaacggagcgccgcacattgtcctttctgtcagatgtgtcagccttggcatacatgatggagattaatgaactatcatgagac
ttgggtggttgcagagaggcttctgtgctttgcagaagggtcttaatcatctgaaaagcacattgactgagctgctattgcttgcaggaggtgacattcc
aactgcaatataaacattttgttttgttttccttgggggggggggatcatttcactgtcttgttattcttttaagcagattattctgtctaacttaaaaa
aaaaaataataattttaccaaagaagtagaggattttttttctcctacttctgattgaaagttaaaaattatttcttttttgattgctgaggtgtattaa
atttaaagaagataatgtctccgtcttctactgtgtaatttctgccttgttgtctgtattttcctgcagccttgacagatgcctctgtgccttcttcttt
gaaatcgtcaaatttcattacttccgccataataaaacccttctcgaaatctgtgtggatcttgcctgcagcctgaggagcctttgtccctttcttgata
gtccaggcacgtacttcatcggggccggcggtgaagaagtactccagttggagtgctgcatatccgagtttgataatcttggataagacactttgtgtca
ttttctccttcaagatacttccgcttttccctcatcactccatggtcttggcaaattggagctcccaagaaccccccttaaaagggaatgaaaccaaggg
caccctgggggtcaatttcttttggtcccaccaccaaatcccctttaatatgtttttaatcaaaccccaatctttggttattta
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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