XB-CLONE-2079832
Sequence:
Full length sequence: BC099265 [+]
AACACTTTAAATTACATAGAGTATATACCAAACTCTGATTTCTAAACAGATAACACAGAAGAAAAGACTTAGTGGAGCACTTACAACATGATGATACATC
TGGTTCTCTTGACTGCAGTACTATTCATTCTACCATTCTCAGAGACCTATCGCATTGTAGGGGGACATGAAGCCAGGCCCCATTCCAGGCCCTACATGGT
GTCACTTCAAACTCCGTTTCAATCTCATTTCTGTGGAGGAACACTTATCCATCCCAAGTGGGTACTCACGGCAGCACACTGCCAGGAAGTCGGGTCATTG
GATGTAACACGAGTAGTTCTTGGTGCTCATAGGCTGCATTTGCCAGACAGATCAGTCCAGGTATTCAGCACACTTAAATCCGTACAGCATCCAGAATACA
ACCCTCAAACCTTCCAAAGTGACATACTTCTGCTTAAGTTAAATGGTTCTGCAAAAATCAATCCTGCTGTAAGAACACTTCCGCTGCCTGTTCCCAATAC
AGATGTAATTCCTGGCACTTCCTGTTCAGTGGCTGGGTGGGGATCCACTTTCAATGCCAGATCTAAATTCTTTCCTTTAATGGAGACAGATGTAGATATT
GTTAACCGAAAGGACTGTAACATAACCTGGCTAGGGGGAATCTCTGAATCTATGCTTTGTACGGCCACTCCTGGGGCACCATTCAAAGGCTTCTGTGATG
GTGATTCTGGAGGGCCACTTGTGTGCAGGGGCCGCGTGGAAGGAGTTGTATCTTTCTCTGGTCAATATTGTGGAGATCCACGCACACCAGATGTGTACAC
TCGTGTCACCTCTTTCCTTGACTGGATCCAAAAAACAATATCAACATTTTGATCTGGTTAACTAATCCATACTTATAATTACAGGTTTGGGACCCGTTAT
CCAGACCTGAGGTTTTCTATATTATGGATCTATCTATCATTTGGGTCTCCATACCTTTAGTCCACCAAAAAGTCATACAAATGTGAATTAAACCCAATAG
GATTGTTTTGCCTCCAATAAAGATTAATTATATCTTAGTTAGGATCATGTACAACATACTGTTTTATTAATACAGAATAAAAGGAGATGCTCTTCCTGTA
ATTTGGAGCTTTGCGGATAATAGATCCAATATAAGTATATACATTATTTCCTTACTACAGTATTCATATTGTCATTTTTTATTATCTTTAGTATCATTAA
GTATAATTAAATGTAGCCTTGGCATTACAACTCTGTTCTTATTTAAAACATGTACAATTCTTATTGATTTCAAGTAACACACCACAAATAATGACCATTT
TTGTAAATAAAAGGAACTTCTCCCTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CB198877 [+]cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggaacactttaaat
tacatagagtatataccaaactctgatttctaaacagataacacagaagaaaagacttagtggagcacttacaacatgatgatacatctggttctcttga
ctgcagtactattcattctaccattctcagagacctatcgcattgtagggggacatgaagccaggccccattccaggccctacatggtgtcacttcaaac
tccgtttcaatctcatttctgtggaggaacacttatccatcccaagtgggtactcacggcagcacactgccaggaagtcgggtcattggatgtaacacga
gtagttcttggtgctcataggctgcatttgccagacagatcagtccaggtattcagcacacttaaatccgtacagcatccagaatacaaccctcaaacct
tccaaagtgacatacttctgcttaagttaaatggttctgcaaaaatcaatcctgctgtaagaacacttccgctgcctgttcccaatacagatgtaattcc
tggcacttcctgttcagtggctgggtggggatccactttcaatgccagatctaaattctttcctttaatggagacagatgtagatattgttaaccgaaag
gactgtaacataacctggctagggggaatctctgaatctatgctttgtacggccactcctgggccaccattcaaaggcttctgtgatggtgaatctggga
gggccacttgtgtgcaaggggcccccntggaaagagntgnnatcttttctctgggcaataatggtgggagatccc
3' EST: CB756770 [+]catgtttttttttttttttttttttttttttttgaagggagaagttccttttatttacaaaaatggtcattatttgtggtgtgttacttgaaatcaataa
gaattgtacatgttttaaataaaaacaaagttgtaatgccaaggctacatttaattatacttaatgatactaaagataataaaaaatgacaatatgaata
ctgtagtaaggaaataatgtatatacttatattggatctattatccgcaaagctccaaattacaggaagaacatctccttttattctgtattaataaaac
agtatgttgtacatgatcctaactaagatataattaatctttattggaggcaaaacaatcctattgggtttaattcacatttgtatgactttttggtgga
ctaaagggatggagacccaaatgatagatagatccctaatatagaaaacctcacgtctggataaccggtcccaaacccgtaattataagtatggattagt
taaccagatcaaaatgttgaaattgttttttggatcccgtcaagggaaagaggtgaacccaagtgtacccactcttgtggtgcgtggattttcccccatt
tttgaccccgcggaaaaatacatctcccttttaacggggcccctggaaaaaaaatgggccctcccagaaacacccattcacaaaaaccccttttgaatgg
gggccccccaagtaattggccccttactaaagcactaaaattccgaaaattttcccccctaaccacaagttatgttgaaaaaatccctttttgggggtaa
aaaaatatctcacatttcgggtctgccctttgaaagggaaaaaaaattttgagaatccggg
3' EST: CB756186 [+]gttcctatattgtggtgtgttacttgaaatcaaccagaattgtacatgttttaaataagaacagagttgtaatgccaaggctacatttaattatacttaa
tgatactaaagataataaaaaatgacaatatgaatactgtagtaaggaaataatgtatatacttatattggatctattatccgcaaagctccaaattaca
ggaagagcatctccttttattctgtattaataaaacagtatgttgtacatgatcctaactaagatataattaatctttattggaggcaaaacaatcctat
tgggtttaattcacatttgtatgactttttggtggactaaaggtatggagacccaaatgatagatagatccataatatagaaaacctcaggtctggataa
cgggtcccaaacctgtaattataagtatggattagttaaccagatcaaaatgttgatattgttttttggatccagtcaaggaaagaggtgacacgagtgt
acacatctggtgtgcgtggatctccacaatattgaccagagaaagatacaactccttccacgcggcccctgcacacaagtggccctccagaatcaccatc
acagaagcctttgaatggtgccccaggagtggccgtacaaagcataaattcagagattccccctagccaggttatgtttacagtcctttccgttaacaat
atccacatctgtctcctttaaagggaagaattttgatctggcattgaaagtggattccccaccagcccctgaacaggaaattgcccggaaattacatctg
gtattgggaacaggccgccggaaaatggttcttacaccagggattggaattttggcaaaacccctttaa
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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