XB-CLONE-2079816
Clone Name: IMAGE:6873832
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Gene:
myh13.S
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Species: Xenopus laevis
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Sequence:
5' EST: CB198835 [+]
cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccgggtggaggtgccact
tctgctcagattgagatgaacaagaagcgtgaagcagaattccagaagatgagacgtgaccttgaagaggccactctgcaacatgaggctactgcctctg
ctctcaggaagaagcatgctgacagtgttgcagaactaggggaacaaattgacaaccttcaaagagtcaaacagaagcttgaaaaggaaaagagtgaact
aaagatggaaattgatgacctagccagcaacatggaaactgtctccaaagccaaatcaaacttagaaaagatgtgcaggactctagaggaccaatattct
gaagtgaagactaaggaggaagagcacctcagggtgattaatgatattaatgcacagagagctcgtctacagacagaaactggggagttttctcgccagc
tagaggaaaaagaatctttggtgtctcaactttccagaggcaagcaagcttttactcagcaaactgaagaactgaagagacaacttgaggaggaaacaaa
ggccaagaatgctcttgcccatggattacaatcagcccgtcatgactgtgacctgctcagggagcaatatgaagaggagcaggaagcaaaggctgagctg
caacggtcactgtcccaggccaacagtgaagtatctcagtggagaaccaaatatgaaactgatgcaatcccgcgcacagaggaactgggaggagcccaga
agaaacttgctcagcgactcccgaaaactggagagccaatttgagccgtccactctaagtgggcttcc
3' EST: CB756778 [+]catgttttttttttttttttttttttttttttttggaatttatttgcatctaagactactaaaggactctttacaaaattactcatattttatgaacatc
ctttgtaaataagttgtaatggtgttgggttcattcttcagctttctttccaacttcccgagtcttggctctgagcttgttaacttgggattctgcgatg
tcggcacgttcctctgcctcttccaattcatgctgagtcttcctgaatctggataggtggacgtttgcttgttcttcaatctcctctgcttgtctcttgt
atgctttgactttcaactgcaacttgtccactaaatcctgcagcctgaaaacattctttttgtcctcttcagactggtaagtcagctctttgactcttct
ctcgtacttgcgcactcctttgatggcatcagatcctctcctttgctcagcatcaagctcattttcaagttcacgtaccctggcttccaatttctggagt
tgcttcttgcctcctttcattgctagttgctcaacctcatctaaacgatgttggagatccttcactgtctgctccagattcttcttcatacgttcaaggt
gagcactggtgtcttgttcctttttgagttcctcagccatcatggcagcatccgtgattgccttcttggcctttttcatctggctttcttgcctcttgag
aaaattccttccacctttgcctttgaaactgaaaaaaattagccctcaatctttcttctttggggtgaatcaaaactggtgttcctgggaaggaaaacaa
cctgtccaaaccttcggtaaaatccaaatgagtttcccgtttct
3' EST: CB756190 [+]tttacaaattactcatattttatgaacatcctttgtaaataagttgtaatggtgttgggttcattcttcagctttctttccaacttcccgagtcttggct
ctgagcttgttaacttgggattctgcgatgtcggcacgttcctctgcctcttccaattcatgctgagtcttcctgaatctggataggtggacgtttgctt
gttcttcagtctcctctgcttgtctcttgtatgctttgactttcaactgcaacttgtccactaaatcctgcagcctgaaaacattctttttgtcctcttc
agactggtaagtcagctctttgactcttctctcgtacttgcgcactcctttgatggcatcagatcctctcctttgctcagcatcaagctcattttcaagt
tcacgtaccctggcttccaatttctggagttgcttcttgcctcctttcattgctagttgctcagcctcatctagacgatgttggagatccttcactgtct
gctccagattcttcttcatacgttcaaggtgagcactggtgtcttgttcctttttgagttcctcagccatcatggcagcatcggtgattgccttcttggc
cttttcatctgcatttcttgcttcttgagaagcttcttccacttcgccttggagctgagagatatcagcctcaatcttcttcttggtgttgatcagactg
gtgttctgtgagtgaaacaactgcacacgctctgtaacatcaatgaatttcctgttctgcccactttgcgcccacccttaaatctggtctaaaaacaaac
ctcatcctcctccaatttccgccagccatgaaggttatttcttccttttcaaaaataaaccaagctggctccct
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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