XB-CLONE-2079696
Clone Name: IMAGE:6873894
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Gene:
rpl8.S
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Species: Xenopus laevis
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Sequence:
5' EST: CB198891 [+]
cgcataacttcgtatagcatacattatacgaagttatcagtcgacggtaccggacatatgcccgggaattcggccattacggccggggctcttcgcggac
agcccggcctagtgcacaatgggacgtgtaatcaggggacagagaaaaggtgcaggctctgtttttaaagcccacgtcaaacacagaaagggtgctgcta
agctccgggctatcgactttgctgaaagaaatggctacatcaagggtattgtgaaagacattatccatgatccaggccgtggcgctcctcttgctaaggt
tgcattccgtgatccttacaggtttaaaaagaggacagagttgttcgttgcagctgagggaatccatactggacagtttgtgtactgtggcaagaaagct
cagctgaacattggcaatgtactccctgttggcaccatgcccgaaggcaccattgtctgttgtgtggaggagaaaccaggtgatcgtggcaaaattgccc
gggcctctggtaactatgccacagttatctcccacaatcctgaaacaaagaaaaccagagtgaagttgccctctggatccaagaaggtcatctcatctgc
taacagagctattgttggtgttgttgctggaagtggtcgtattgacaaacctatcctgaaagcangtcgtgcctaccataaatacaaggcaaagagaaac
tgctggccacngtgtccgtgggtggtggctatgaacccctgttggacatcccctttccggtggggggggaacccatccaacacatttggttaagccccct
ccaaccaattcagggagaag
3' EST: CB756838 [+]catgttttttttttttttttttttttttttttttacaatttattttgtaattattagatagacaagctttagttctccttttcctgcactgtctttgtac
cacgcagacgaccggtacgacgagcagcaataagaccaactttgcgaccagctggggcatctctcctgatggttgagggcttaccaatgtgttgatggtt
accaccaccgaagggatgttcaacagggttcatagccacaccacggacacgtggccagcagtttctctttgccttgtatttatggtaggcacgacctgcc
ttcaggataggtttgtcaatacgaccacctccagcaacaacaccaacaatagctctgttagcagatgagatgaccttcttggatccagagggcaacttca
ctctggttttctttgtttcaggattgtgggagataactgtggcatagttaccagaggcccgggcaattttgccacgatcacctggtttctcctccacaca
acagacaatggtgccttcgggcatggtgccaacagggagtacattgccaatgttcagctgagctttcttgccacagtacacaaactgtccagtatggatt
ccctcagctgcaacgaacaactctgtcctctttttaaacctgtaaggatcacggaatgcaaccttagcaagaggagcgccacggcctggatcatggataa
tgtctttcacaatacccttgatgtagccatttctttcagcaaagtcgatagcccggagcttagcagcaccctttctgtgtttgacgtgggcttttaaaaa
aagagccctgcacctttttctctgtc
3' EST: CB756127 [+]ttcaaaactgcttttcctgcactgtctttgtaccacgcagacgaccggtacgacgagcagcaataagaccaactttgcgaccagctggggcatctctcct
gatggttgagggcttaccaatgtgttgatggttaccaccaccgaagggatgttcaacagggttcatagccacaccacggacacgtggccagcagtttctc
tttgccttgtatttatggtaggcacgacctgccttcaggataggtttgtcaatacgaccacctccagcaacaacaccaacaatagctctgttagcagatg
agatgaccttcttggatccagagggcaacttcactctggttttctttgtttcaggattgtgggagataactgtggcatagttaccagaggcccgggcaat
tttgccacgatcacctggtttctcctccacacaacagacaatggtgccttcgggcatggtgccaacagggagtacattgccaatgttcagctgagctttc
ttgccacagtacacaaactgtccagtatggattccctcagctgcaacgaacaactctgtcctctttttaaacctgtaaggatcacggaatgcaaccttag
caagaggagcgccacggcctggatcatggataatgtctttcacaatacccttgatgtagccatttctttcagcaaagtcgatagccccggagctttagca
gcacccttttctgtgttttgaacgtgggcttttaaaaaaaagaaacctggcaacctttttctcttgtccccctgaatttaaacgttccccatttgtggaa
ctaagggccccggggcttgttccccccgaaaaaaagacccccggggcccgcttaaattgggct
Library:
Description: 5' and 3' adaptors were used in cloning as follows: 5' adaptor sequence: 5'-CACGGCCATTATGGCC-3' and 3' adaptor sequence: 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)BN-3' (where B = A, C, or G and N = A, C, G, or T). Average insert size 1.7 kb (range 0.8-3.0 kb). 15/15 colonies contained inserts by PCR. This library was enriched for full-length clones and was constructed by Clontech Laboratories (Palo Alto, CA). Note: this is a Xenopus Gene Collection library. Vector Details | Vector Map |
Name: pDNR-LIB
Type: plasmid
5' Restriction Site: SfiI
3' Restriction Site: SfiI | |
Usage:
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