XB-CLONE-1997754
Clone Name: XL006o16
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Gene:
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Species: Xenopus laevis
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Sequence:
5' EST: BJ031356 [+]
gaatcctgtacagaacagatacagggnaatagatgtacctgattctaacatggagacctatatctgttctgagtgcaataaacactttagaactgagact
ggtcttctcaagcatcacaaaactcacacaggagcaaaaccatttgtctgttctgtgtgtgagaaacgttttgtgtctaatgcaaagcttattgtacatc
agagaagtcacacaggagagaaaccgttcccttgtcctgaatgtgggaaaagttttactcgtcgctcaaaccttaaggatcatttaagaggacacaccgg
ggagaaaccgtattcttgtgctgtgtgtggggagagtttttcttatcaatcaacctttaacagacacttcagggtacacacaggagagaaaccattttct
tgctcccaatgttataaacattttacgtgtcaagcaactcttacggcgcatcaaaaaagtcacggagtgaaaaaaccatactcctgttccgaatgtggca
aatattttgctcagcgctctaaacttgcagagcatcaccgaactcacacaggggagaaaccattttcctgttctgattgcggggcaaggtttacctatcg
ctcactcttaaggagacacaacaaaatccacgcagaagggaaatcgttaatttgctccgaatgtgggaaaccgttcacatctgagtcagctcttactgca
catcaaaggagtcacnggagtggagaaaccgntctcttgcaccgactgtgagaaatggtttgctcagcgcatgcatcttattgagcaccagaggactcac
acaggggagaaaccattttcttgtnccgngtgtggggnaatggttacatatcg
3' EST: BJ046291 [+]aataaatgcgtacaattttattaacagtgaatnttgtactttggtagaatctgcttgtatgttactcagtctttactgtaaaagtactgttatcagacaa
tggtttctcccctttgtgtaactgggaatgcgcaacaagaagggaatgtcgtgcaaaacattttccacatttagtacaagtgaatagtttgcctcctgtg
tgaactctgagatgttcctttaggtgtgcaatacgggtgaaacattgcccacattcggtacaagtgaacggtttctcccctgtatgaactctaaggtgga
ctctaaggtctgaggcccagataaaacgtttcccacattcagagcaaggaaacggtttgtctcctgtgtgtattctttcatgtgctttaagatgtgactg
atcactgaaacatttcccgcactcggaacaaaagtgcgatttctctcctgtttgaactgtgctatgtgcagggaggtcagagccacttgtgagtggtttc
ttcccgttgtgaattctctcgtgggttttgagggctgagcgttctttaaatgttttcccacattcagaacaagaataaggtctctctccagtatggactc
tctgatgtgaagcaagttgggactggtatttaaaacatttcccacagtcgctacactggagtggactcttccctttatgactttttcgatgtacagtgag
atctgagcga
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: