XB-CLONE-1993149
Clone Name: XL022n01
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Gene:
prelid3a.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ033777 [+]
gcaactctggtacttaccctctgcttcatcactgggatcagagcttccccagtgccaccttctacttcttgctcacccccttgtgtgcagacaccaccac
ctctgaaaggaagtacaggcagtgcagacaataattctcagaacacaaactgctcctggtcccagtggtttaatgtctacagaccaacatccggcccaga
tggtggagactttgagacttttgagaacataagggctgaagggcatgctgtatgtagtgcacctcaagcagtgagttgtaggctagtcagatttcccgat
atccccttggataaactgtactacactcaggaatgtggggtttctcaaggcttggtttgtgaaaataaaaatcaaggtaatgacagctgcttggattatg
agatcaagattttatgttgcacaggccagttggctaatgctacagaatccacaaaaattactacaatattgccccccaataataatcctgaagctactcc
actatttgatgaacaaggaagttcctctgggatggaagaagatgtttccacaacagttatgatgacagtgttccctaacaataatgaaactactccgata
tttgatgaacaaggaagttcatcccggatggaagaagatgtttccacaacagttatgatgacagtgttccccaacaataatgaaactactccgatatttg
atgaacaaaggaagttcatctgggatggaagaagatgtttncacaacagttacgatgatagtggtncctaaaataatgaaactactncgatatttgatga
caaggaagttcatctgggatggaanaanatgcttccncaacagntacgaggacagnggtccctaacaataatggaactactncgattttgatgaacna
3' EST: BJ049027 [+]tcggttggattctctttagaaaccgagtggattataagatttaaacattaagccgaagaatattactttttatttggaagcattttgaatgtgccaagct
ttcttctcaatgtagccatcctatggtgtcctaaattattttttggggggcactgttttagtaattgttggtgaaggctcaggagagttgctttcttcca
tgccagatgagcttccttgttcttcaaataatggagtattttcattattgttagggaacactgtcatcttaactgttgtggaagcatcttcttccatccc
agatgaacttccttgttcatcaaatatcggagtagtttcattattgttagggaacactgtcatcgtaactgttgtggaagcatcttcttccatcccagat
gaacttccttgttcatcaaatatcggagtagtttcattatttttagggaacactatcatcgtaactgttgtggaaacatcttcttccatcccagatgaac
ttccttgttcatcaaatatcggagtagtttcattattgttggggaacactgtcatcataactgttgtggaaacatcttcttccatccgggatgaacttcc
ttgttcatcaaatatcggagtagtttcattattgttagggaacactgtcatcataacctgttgtggaaacatcttcttccatcccagaggaacttccttg
ntcatcaaatagtggagtagcttca
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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