XB-CLONE-1930115
Clone Name: XL052j04
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Gene:
zscan29.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ069332 [+]
ggnatgcagcctagtgcagnaaacgttatacaacacaaatggcaacaataaaaatgataaaacatccgcacattttagtcataatagagagtttgagaga
catgaaagaacccacccaggaaaaaaactttcattttttacttgtacagaatgtgggaaatgttttgcctcttcatcatcactcacagtccatcgacgac
aaatccacacaggagaaaaacctttttcttgttctgaatgtgggaaatgttttgcctcttcatcacaacttactgttcatcgacgacagattcacacagg
agtgaaacctttttgttgttcagaatgtgggaaatgttttgtcactacatcacaacttaatgtccatcgacgacagatccacacaggagagaaacctttt
tcttgttctgaatgtggaaaatgtttttcatttcaatcacgccttanagagcatcaaaaaatccacacagggcanaaacttttttcttgttcagaatgng
ggaaatgntntttaagccaatctcgccttanagcncntnncanaatncncncnggnnaanaaccttnnnnttgnnnngnnngnnnnanangnntnt
3' EST: BJ086273 [+]gttctccccggtgttgatttattgcacatgaacagattggatgaagcatcaaaacacacattatgatataatcgatgggaacgaaatatttgcctgatgc
ctgatttgtaaaacatttcacacaatgacaataagcttacattttttttcctgtgtgaatcttaaaatgtatttttagactactgctatgactaaaacat
ttcccacattcagaacaagaaaaaggtttctctcccgtgtgggttctgtgatgaattgtaagatttgattggtagaaaaaacatttcccacattctgaac
aagaaaaacggctctctcctaagtggactcgttgatgatgggactgaagtcctgatgaattagcgaaacatttcccacattttgcacaagaaaaaggttt
ttctcctgtgtggattcgtcgacaatggacggtaagttgtgatgaagtgacaaagctttttccacattcagaacaagaaaaaggtttttctcctgtgtgg
attctgtgatgtgctctaaggcgagattggcttaaaaaacatttcccacattctgaacaagaaaaacggctctctcctaagtggactcgttgatgatggg
actgaagtcctgatgaattagcgaaacatttcccacattttgcacaagaaaaaggtttttctcctgtgtggattcgtcgacaatggacggtaagttgtga
tgaagtgacaaagctttttccacattcagaacaagaaaaaangtttttctnctgtgtggattctgngatgtgctctaaggcgagattggcttaaaaaaca
tttnccacattctgaacagaaaaaagnttctgncctgtgnggattttttgatgctctcttaaggcgtgattgnaatggaaaaacatttttcacatt
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: