XB-CLONE-1910603
Clone Name: XL141l05
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Gene:
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Species: Xenopus laevis
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Sequence:
5' EST: BJ093193 [+]
ggagctctgcaggctaatgaggagactctgcccaaggacaggagagaagaaggagttgcagaagatcccagagcagcattaagcagcagaggttccaagt
aagagaatccagggatttctgggattccttagatccagccaaggagccgttacaggtgcaggacatagaataaagcagagacaccgtgtgactcaggaat
aggatatcatgtctctcagcaaaccaaatagcagcagatcttcttgtctgtcagttacaacaagtcgtaagacaaaagaggccgaggtgcaactttgtag
cacaatggaatgtgagcagcaagacaacagtcttacaggacatcagaaggttatcaaagaagagaaacctttcccttatgaagaatatggcaaagtcttc
tctccaaatgtgacacttcttatacatcacacagagcagaaaccattctcatgtaatgaatgcggaaaaggcttttctacaaagcagaaactcaaaattc
accagacagttcacacaggggagaaacccttcaaatgcacagaatgtggcaaaggcttttccacaaaacatcagctcaaacttcaccaagccagttcaca
caggagagaaacctttcatgtgcgctgaatgtggaaaatgcttttctacaaancaacaacataaaattcaccacatgactcacacaggggagaaaccctt
cacatgcacagaatgcagcagangcttttttacaaaacgcgatctcaaaatgcaccagacagttcacacaggggagaaaccccttcacatgcacagaatg
ncgncnaanggtttttntacaaagcaaaagctcaaaattcatcaaatagttcacacanggggagaaaccccttcnaatgcnccanaannggccaaag
3' EST: BJ097429 [+]actgtacctncatacagtagaattctgatatattgtttacaaaacaaaagctgcagtgcaggatacaaataactctagaatgaattacagctatttattc
agggatgatggaatgttgtttaggattacatgttctttcatacagcacacatttaggggttctcccctgtatgagtcatctggtgcattttgaggttttg
ctttgtagaaaagcctttgtcacattctgtacatgtgaagggtttctgtcctgtgtgaacagtctgatgaattttgagattatcctttctagaaaagcct
tttccgcattctgtgcatgtgaatggtttctcccctgtgtgagtcatgtggtgaagattgagcttctgctttgaagacagacattttccacatgttgtac
acataaagggtttctcccctgtgtgaactgccaggtgaattttgagcttctgctttctagaaaagcctttgccgcattctgtgcatgtgaagggtttctc
tcctgtgtgaactgtctggtgaattttgagtgtcttctttgtagaaaagcatttgtcgcattctgtgcatgtaaagggtttctcccctgtgtgaattgtc
tggtgaattttgagtggctgcattgtcgaaaaacatttgccacattctgcacacatgaatggtttctcccctatgtgaactgcctggtgaagtttgagct
gatgctttgtaggaaangctttgccacattctgtgcatgtaaanggtttctntcctgtgtgaactgtctggtgacntgtgancttctgcattgtccaaaa
nccttatancattctntccatgtgaaaggntttctnccctgtntgaaccatntggngaattttganctnatgcctttgnaaaaaatcctttgctgcattt
tnnncatttgaaaaggttttnacctgtggaact
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: