XB-CLONE-1910569
Clone Name: XL141m15
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Gene:
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Species: Xenopus laevis
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Sequence:
5' EST: BJ093223 [+]
cgggctgcagggnagagcacaatatccttgagcctcgtgcctgtttatggtnatggaactccttggtaacttataatatattttttcactatatagtact
caagagaacacaatatccgtggatttgcatatgtttcacatggcagagtcactatttgtcaccaaacacccttctcccttaattgccatctcactaactg
ccatctctccaaaaatagtcatcaacatgaattcctacttgctaaacaagcatctcctgcactgctgtctccccatacaggcacccacactacaaaatga
ctcaatttcaagctcataaatgtaatcacgctgcaaagaaaataaggcgtttaaaacaaattaaaagtttaactcattgaatcaaaatgaatcatcaaag
aatacgcccagatgctatttaaattcctgctatatttcatgatagtgtttcgtaagataacatatgcaaagtgatgtcttttcaaaatctaaaagcattt
acttgtctcatgccatgcatacaacactgcctagataccaaaataagagttttgattctcccataaggcatcatgggcagagacatgcatattaatcctt
tatgtccctttggccaactctgcatccagaaccactgatttatagcacagatgcagtcttatggttcagaaccatatatccaaacttgcagacaaactgc
tttgatcttatcaatgcaagatattggaacattggttttgagangtaggacttggagagtagctaaatgggaaaccaaacctggntangggggagaaggg
ctnaaagagaccaaagagaccctttactnacaatttacatggcnaagngaagccttctgnaatcanaaggnggtttgcttggtntngnggcatgcaacan
aaactggnctanataccaaaanaaaaantttggattctnccataaggcatcatgggccggganatg
3' EST: BJ097446 [+]ttttttgtttttttttagaaatngaaggtatctacttgtctcctgcctacacacagcactgcctagacaccagnataggatgtcctaaaatgcgttctgt
agaaacagcctgtctgcaagtttggatatatggttctgaaccattaggctgtatctgtgctataaaccagcggttctggatgcagagttggccaaaggga
cataaaggtgtgatttgcatgtctctgcccatgatgccttatgggagaatcaaaactcttattttggtatctaggcagtgctgtgtgcatggcacgagac
aagcaaacaccctctgatttcagaaggcttcactttgcatgtaaattgttagtaaaggtctctttggtctcttttagcccttctcaccctaaccaggttt
ggtttcccatttagctactctccaagtcctacctctcaaaaccaatgttccaatatcttgcattgataagatcaaagcagtttgtctgcaagtttggata
tatggttctgaaccataagactgcatctgtgctataaatcagtggttctggatgcagagttggccaaagggacataaaggattaatatgcatgtctctgc
ccatgatgccttatgggagaatcaaaactcttattttggtatctaggcagtgttgtatgcatggcatgagacaagtaaatgcttttagattttgaaaaga
catcactttgcatatgttatctta
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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