XB-CLONE-1910455
Clone Name: XL141p23
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Gene:
ube2k.S
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Species: Xenopus laevis
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Sequence:
5' EST: BJ093293 [+]
tggcaacgtatatatttgtactttacagggcaaccaaacctgcattttgtttttcctactcaccccacccgtaagctccttagtttaaatgaaggcatcc
aggggatattaacctgcttttgagtaggttttatttttcagtgagctgtagttagcatagcttattctgtgcacaaatacaccctgatatcatcgtatgt
atagaaatgggagctgccacattgcttccttcatgtgaggggttctctatatccagctgacagctgccatgttgttattattaagcattgcccatttgtg
gtgtgcaaaataagctatgctatcttaatctagagtagggatgcaccgagtccactattttggattcgggcgaatccttcgtggaagatttggccaaatt
ctgaatttgaatatgcaaattagggttgggaaggggaaaaacattttttacttccttgttttgtgacaaaaagtcacttgatttccctcgccgcctggcc
aggcagaaggattcggccgaatcctgctgaaaaaggctggattcggtgcatccctagtcctaatctagattagagatggactgaatccattattttggat
tctgctgaaccctcaaatcctttgtgaacgatttggccaaatactgaactgaatcctaatttgcatatgcaaattangggtgggatggggaaaacatttt
ttacttccttggtttgngacaaaaagtcacttgatttccctnctgnccgggcaagccgaatnctaatttgcatatgcaagntangattcngntcanccgg
gcanaaggcttcagccnaatcctgctgnaaaagctggatnc
3' EST: BJ097503 [+]ttattacggaagaggaattttagtctacaaattttggattgaattattcggcttttagaagtgtgttgtaacaatgacagggatttcccccctgaagtgc
tagtgttgccaggaaggatttgccgtgatctgacgcccaacggtggaattatgtttacagcccatttgccgacggatgagtcttaaatagtaaagtactg
tacagtgcattttgacaggtttgccttctcattatttggctccttccattaaaactgactgcagtctttcatctagctctaatcatgaagcttaaagatc
aacaaatccagtttccacaccacctttaaccacttggtggtctctggcatattgcagtgcaaagctttggccatcttcttgcccccaagattgaaaatca
ggtaaactgtgggttcactgagccctaaaaacttctgattattctgatagctacacccccctctccccaaatgatatatatgagctcatggtacggaata
tactacagatgaatggagcaaattcattagcgcacatgcatgcaagtccacaggcacaaatgatggccgctactaacggtaaggtagacaatggctggca
aaatggatggcaattaaaacttaaagaggaaatacaccccgttctgggtgttccttttatttccccctaa
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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