XB-CLONE-1909657
Clone Name: XL143k24
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Gene:
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Species: Xenopus laevis
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Sequence:
5' EST: BJ093845 [+]
acaatatcnttgagcctcgtgcctgtttatggtcatggaactccttggtaacttataatatattttttcactatatagtactcaagagaacacaatatcc
gtggatttgcatatgtttcacatggcagagtcactatttgtcaccaaacacccttctcccttaattgccatctcactaactgccatctctccaaaaatag
tcatcaacatgaattcctacttgctaaacaagcatctcctgcactgctgtctccccatacaggcacccacactacaaaatgactcaatttcaagctcata
aatgtaatcacgctgcaaagaaaataaggcgtttaaaacaaattaaaagtttaactcattgaatcaaaatgaatcatcaaagaatacgcccagatgctat
ttaaattcctgctatatttcatgatagtgtttcgtaagataacatatgcaaagtgatgtcttttcaaaatctaaaagcatttacttgtctcatgccatgc
atacaacactgcctagataccaaaataagagttttgattctcccataaggcatcatgggcagagacatgcatattaatcctttatgtccctttggccaac
tctgcatccagaaccactgatttatagcacagatgcagtcttatggttcagaaccatatatccaaacttgcagacaaactgctttgatcttatcaatgca
agatattggaacattggttttgagaggtaggacttgggagagtagctaaatggggaaaccaaacctggttaggggggagaagggctaaaagagaccaaag
agacctttactaacaattacatggcaaagtgaagccttctgaatcaganggnggttgctttgnctcgngcatgccacananactggctanataccaaana
agagntttgatttttccataaggatcatgggcagannatgcnaatacanccttttgttcctttgg
3' EST: BJ097903 [+]aagannnnatatatggttctgaaccattaggctgtatctgtgctataaaccagcggttctggatgcagagttggccaaagggacataaaggtgtgatttg
catgtctctgcccatgatgccttatgggagaatcaaaactcttattttggtatctaggcagtgctgtgtgcatggcacgagacaagcaaacaccctctga
tttcagaaggcttcactttgcatgtaaattgttagtaaaggtctctttggtctcttttagcccttctcaccctaaccaggtttggtttcccatttagcta
ctctccaagtcctacctctcaaaaccaatgttccaatatcttgcattgataagatcaaagcagtttgtctgcaagtttggatatatggttctgaaccata
agactgcatctgtgctataaatcagtggttctggatgcagagttggccaaagggacataaaggattaatatgcatgtctctgcccatgatgccttatggg
agaatcaaaactcttattttggtatctaggcagtgttgtatgcatggcatgagacaagtaaatgcttttagattttgaaaagacatcactttgcatatgt
tatcttacgaaacactatcatgaaatatagcagggaatttaaatagcatctgggcgtattctttgatgat
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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