XB-CLONE-1909279
Clone Name: XL144i10
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Gene:
hspa12b.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ094133 [+]
gctgggtacccctggnaactatagcagggtgactgttaccccaatgtttctatatatctgtaaccttgttatgagctaaagggaaccagcctgaaggcca
gttagggggagatttggggtgagagcttatttgtgccctgggtacccctggaaatatagcagggtgactattaagctggccatagatgtaaagattttca
aaagatccgatcgtcatcgtgagaccacgattatctcaaaacgattgtacgatcaccatttcaggcgatattgccaggaaaacaaagggtagctgcctgc
ttgtccctgcaaacatagatagattgcactggggctgataaagatttttttaaatcaattttctgacagatgtcggccgaaaaaatcgtaagatgtacag
tcgttcgaatcccactaaccgcacgattggtcggacttcgctaaaatcggtcctttggcaagagaaatctttgcgtctatgggaacctttaccccaatgt
ttctatatatctgtaaccttgttatgagctaagcgggcccagcctgaaggctggttagggggagatttgggggtaagtgctgatttgtgccctgggtacc
cctggaactatagcaggctgactgttaccccaatgtagggatgcaccgaatccagggattcggttcgggatttggcctttttcagcaggattatgattca
agcccgaatccttctgcccggctgaaccgaatccgaatttacatatgcaaattangggcggcagggaaattgcgtgactttttgtcacaaacaatggaag
tgttcggtattcggctgaatncaaaatagnggatcaagngcattcctacccccaatatttctatatatatatctgtaaccttatgagctaaggggnccag
cctgnaggccagtntgggggagaattgggggngagngcttat
3' EST: BJ098092 [+]gatgattagagaactgacacttggagacgccgcaagagattaaaccggagagagaatcccagagatnacagttagtatttacaatacatttgttttggca
gggtatagacatttgtgaagaagaggggtgtaaaaggaaattgccagcccattataacaggcctctggggcaggttacaaatcatgttcctttgtgaact
gtagcatccatttcctttccctacgctaaggttaaaaagtagagaagaggctcagacagtaccatgtaacacacagaaaactcccaaattcactatgggt
aaaggaggagacaagggaaccaatgctttttgggaggaatttaaatggaatacaacagctccccctgctggtcagtatcaggaatatgagggaatgtggt
cagcctacttcttgatatatagaaatattggggtagcagtgaccctgctatagttccagggtacaaataagcactcaccccaaatctccccataactggc
cttcaggctgggccccttagctcataaggttacagatatatatatagaaatattgggggtagggatgcacttgatccactattttggattcagccgaata
ccgaacacttcattgttttgtgacaaaaagtcacgcaatttccctgccgcccctaatttgcatatgtaaattcggattcggttcagccgggcagaangat
tcggctgaatcataatcctgctgnaaaaaaggccaaatcccgaaccgaatcctggatttcggtgcattccctacatttggggtaacagtcagcctgctnt
agttccanggggtacccanggcacaaatcancacttacccccaaatnttcccctaaccanccttcaagctgggcccgcttanctcataacaaggttacag
atntnta
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: