XB-CLONE-1906026
Clone Name: XL151l06
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Gene:
cyrib.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ095838 [+]
tgcaggnaattcggcacgagggttgacttaaaattatacatatacctgttttctggccaaggttgatgttgaggtgatccccaatagagctctggaaggc
atctccagaaagacgtggcaggtgatgacacacttttcttggtcaaccacaagtggagacctttaaaaatggtcaaacaagagttcccagagatcattga
ctaacatcacacatatacctggtttctggctgggattgatgttgaggtgatccccactagagctctgaaggcatcttgacacacttttcttggtcaacca
taagtggagacctttaaaaatggtcaaacaagagttcccagagatcattgattaacatcacacatatacctggtttctggctgggattgatgttgaggtg
atccccactagagctctgaaggcatcttgacacacttttcttggtcaaccataagtgaatacctttaaaaatggtcaaaccagagttcccagagaccctt
gactaaaattaaacatatgcctgattttggttttgaatgatattgtgtgatcccaactggagccctggaagccatctcctgaaagtcatggcagctgatg
acatacttttcttgctcaaccacaagtggagacctttaaaattggtcaaacaagagttcccagagatcagtgacttaaaattatacatatacctgttttc
tggctgggattggtggttgaggtgattnccactggagctctggaaggcatctncagaaagtcgtagcagatgatgtcacacttttcttggtcaccacaag
tggagatcttttaaaaaagggtaaacaagagttcccagaaatncatgacatacaatatcatttacctgggttctggctgggattanggtgggatgatccc
c
3' EST: BJ099719 [+]aagctgatttttattttatttttttttgtttacagcccccattggacataaacatgcatacgtaatcgttacgcacacatcacatggtttaatgttagtc
atcaaagattaaaacatacaacatacatccttacaaaaatgagtccaaatgcaaccagaaaactctttgcttcactaaggaatccatgaaaatcattgtg
ctgggtaccattttgtttgttttttaatttatacaacacagttaactgatacaaatctgctcgttgtcggaggctaatagcccaggtggggttctgacca
agttaaaaaaaatgtggcatcatacgccatgaggttctggagatcaccttcaggtgtttcaaagagcaaggtatcaacatcgattatcaccagaaaccag
gtatgtgtgtgcttttagtcatgggtttctgggaactctcctttgaccatttaaaaggtctccacttgtggttttgaccaanaaaaaaaggtcatcatat
gccactactttctggagatgccttccagagactgcagtggggatcatagatcaacctgaaaccaggtatatatatgatcgtaagtcaaggatctctggga
actcttgtttaaccatttgtaaaggtctccacttgtggttgaccatgaaaanggtgtcatcatctgacattactttctgagctctagtggggatcatcac
aacattaatcccagccagaaaccaggtaaatgtatattgtatgtcatggatctttgggaactnttgtttaaccattttttaaanatctncactttgnggt
tgaccaaaaaaagtgtgacattatntgctaccacttttttggaatgcctttcananctncagtgggaatna
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: