XB-CLONE-1904972
Clone Name: s1pr5-LJ115
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Gene:
s1pr5.L
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Species: Xenopus laevis
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Description: EXRC Clone Number LJ-115.
Xenopus laevis sphingosine-1-phosphate receptor 5.
Full length clone.
To generate mRNA for injection and Probe for in situ.
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Sequence:
5' EST: BJ097268 [+]
ggacgctgctgccgggataaggtgccaggctgcaaagcgatgctgaaacgctggactaacagaatacaaggagttactgccatctagtgatctcgtcttg
ttagtgcagcattatacagcggctgcctctgctgtaacatgttgtaaatccactcacaaagaggaattcatttatttcagtgtgtgtggccatgaagaca
aaaggggagttattcaaaagagtagcatctggagtctttagctgggactttttctgccaccatctgtcggagcaacctgcttacacctcacagactcttc
tataaagcagtctactttggaaggataccgaatatcatttcagagatctcctcttagtaatgtttttggcccccagataagagacgtttgggcttgtaaa
aagaaataaaggattgacgaaaccatggatcttctgcagccccccaatcgactggtccgattgtacagagagtaccagaacaatgaagtcatctctctgc
actacaacttcacaggaaagctcaatacaagcaggtacaagggaggactaaaggccgaggctgtattctttttggtggtttgtgtcttcattgtcttgga
gaacctcattgtgctcttggccctctggcgcaaca
3' EST: BJ100247 [+]aatgttatgtttgagtnttaaaaactgaggaatgagagctgattgcacatggatacagggcagtgattggctgttccttttagggttgcaccgaatccac
ttttttggattcggccgaactcccgaatccttagcgaaaaattcggccgaataccgaaccgaatcggaaccctaatttgcatatgtaaattagttgtggg
acggggaaaacattttttacttccttgttttctgacaaaaagtcacgtaaatgcccttttttggatttggccgaactcccgaatccttagcgaaagattc
ggccgaataccgaacccttatttgtatatggattcggtgcatccctagttcctttacctgtagccatcatggcttcttcggtgttctttagatgcatgga
agtcagtcccacaaataagaccaacagacccaataggaagcaataaaacccccccagctataagtagcagctatggtgtgggccgtatgataataaaagt
tatgataataattgcaaagaatataacacagcagtcccagtcatatgtaataattcacttcgtgttttatatacagataatacatacaaaatatacactt
gagtctcagtaacaaaatcacataaaggttaaaacctttctaaatggctgctactgcttcacacccgcttacgataggctgatattctc
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
RNA injection:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
sense |
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T7 |
XhoI |
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Whole mount in situ hybridization:
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
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Suppliers:
Search for clone at:
EXRC