XB-CLONE-1904950
Clone Name: XL152a21
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Gene:
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Species: Xenopus laevis
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Sequence:
5' EST: BJ095935 [+]
gcacgaggaaaaaataggggacgctccccacctgcaacatatttggtgtctgtgcgattctctttctactttccactgaatgcagagttcaacacattta
atctcgagacttccgaaaacaccaatataaagcactatccattgaggatcctcgacttaatatgtggactcgtgggtgtaatatctattagctacctatc
tacatatgtctggtcaatgtttagccataagaaaaaagaagaagacgatgtggagatgcaaaataacccagatgttttattggaagctcctgttgaaaag
cagactgtgatccttatacctggagcatgtgatttaactaatattgtggctactgacgtaaacacggaaggaattgaggaaaataaagtgaatcctgtgg
ccactgaagagccacaggacagataactgagccaaatgaggagcttcaacagaaaccagaacaggaagctgagttaaagaaaaaaaagaaaaggaaattt
tttttcttgaaaggcaacgatacattacaatagcaacgatacataacaatactacggaaacaaataaataagacaggaactctacatgagtctgtgatcc
aaaagccactctacctgtgtcacagcgtttcccattcagttgaatgggaattgggggttgtcttcattgtttaacaacaccaaagatnactgagccaaat
gaggagcttcgacagaaaccagaacaggaagatgagttaaagaaaaaaaagaaaagaaatttttttttnttgnaaggcaccgattccttntttnacaatt
ccttggggaaacaaatnaataagnccagggacctctacatggagtctgggatnccaaaagccnct
3' EST: BJ100258 [+]tttgttgttgttaaacaatgaagacaacccccaattcccattcaactgaatgggaaacgctgtgacacaggtagtggcttttggatcacagactcatgta
gagttcctgtgttatttaattgtttccgtagtattgtttaaagatgtatcgttgcctttcaagaaaaaaaaattccttttctttttttttctttaactca
gcttcctgttctggtttctgttgaagctcctcatttggctcagttatctttgttgttgttaaacaatgaanacaacccccaattcccattcaactgaatg
ggaaacgctgtgacacaggtagtggcttttggatcacanactcatgtanagttcctgtcttatttatttgtttccctagtattgtttaaagatgtatcgt
tgcctttcaanaaaaaaaaattccttttcttttttttctttaactcaacttactgttctggtttctgttgaagctcctcatttggctcagttatctttgt
tgttgttaaacaatgaanataacccccaattcccattcaactgaatgggaaacgctgtgaaacaggtagtggcttttggatcacanactcatgtanagtt
cctggcttatttatttggttccctannattggttaaanatgtatcgttgccttttaanaaaaaaaaatttcttttcttttttttctttaactcatcttcc
tgntctgggttctggcnaaactncttatttggctaagttatctttggtggtggtaaacaatgaaaacaacccccaattnccattcaactgaaangggaaa
ccttgnncnccaggngnaaggggcttttggannacaaacttatggaaaantcccggcttatttatttggttccc
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: