XB-CLONE-1870956
Clone Name: XL185m24
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Gene:
lsm2.S
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Species: Xenopus laevis
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Sequence:
5' EST: BJ618613 [+]
gcacgagggtgatattttctaacggtagccggtctgttctatagtttccagccagagttttgttggcgccactgcagcaggaacgatgctattctactcc
ttcttcaagtctctggtagggaaggatgtggtggtagaactgaagaatgatttgagtatctgtggtactctacactcagttgatcagtatttgaacatta
agctgactgatatcagtgtaacggacccagaaaaatatccacacatgttgtccgtgaagaattgtttcatccgtggatctgttgtgcgctatgtacagct
cccggctgacgaagtagacactcagcttctccaggatgctgcaaggaaagaagcagttcagcaaaagcagtgacctacaagagctggaagggtgaatgca
ggttcacaagcattgataggtgttgtcaactgcctgtttctataagaccatgtaaaagagtgagcagcaacattttacctgttatacaggttttgtgtaa
gagtcacacattttttccccttcgaatttctgaagcttcatgtgcaatgacttgtgatgctgttcacttttgaatccaagcaatgcaaagaagtggtctc
cgataaacagatttatttgggcaagactgagccccaaggtttttgttctttttttctaaataaaggatatgttaacatttatcagatcttgttatttagc
aaatctagaatctctttatgtatatgaatttctttattttattgaacccaaaccctatat
3' EST: BJ635193 [+]gtttgggttcaataaaataaagaaattcatatacataaagagattctagatttgctaaataacaagatctgataaatgttaacatatcctttatttagaa
aaaaagaacaaaaaccttggggctcagtcttgcccaaataaatctgtttatcggagaccacttctttgcattgcttggattcaaaagtgaacagcatcac
aagtcattgcacatgaagcttcagaaattcgaaggggaaaaaatgtgtgactcttacacaaaacctgtataacaggtaaaatgttgctgctcactctttt
acatggtcttatagaaacaggcagttgacaacacctatcaatgcttgtgaacctgcattcacccttccagctcttgtaggtcactgcttttgctgaactg
cttctttccttgcagcatcctggagaagctgagtgtctacttcgtcagccgggagctgtacatagcgcacaacagatccacggatgaaacaattcttcac
ggacaacatgtgtggatatttttctgggtccgttacactgatatcagtcagcttaatgttcaaatactgatcaactgagtgtagagtaccacagatactc
aaatcattcttcagttctaccaccacatccttccctaccagagacttgaagaaggagtagaatagcatcgttcctgctgcagtggcgccaacaaaactct
ggctggaaactatagaacagaccggctaccgttagaaaatatcacc
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: