XB-CLONE-1864404
Clone Name: XL198j16
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Gene:
tsnax.S
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Species: Xenopus laevis
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Sequence:
5' EST: BJ621770 [+]
cggcacgaggctaaaggttcgggatcatttaaaaaccgcaaacctgatatttttcaaagatcacaaagaaaagatgagaaaggcagtgttcacagttcct
cagcagtagtaatggcctttaaagattttcaaagtgaactggacgcaaggcatgataaatatgaacgacttgtgaaacttggccgggatataacgattga
gagcaaaaggacaattttcctgttacacagaattatgagtgatcataataaagaagatgtattgtctgaagcagagactaaattacttaccgttcgtcaa
aaaataagagaaattgcagaggagttggtgggagaagatatgtatcagtatcaccgggcatttacaccaggtcttcaagagtatgttgaagctatcactt
tcaaacatttcattgagtccagaacacttgttaccatcaatgaaattaacaagcagttgatatttgaaggcttggaaaacatgcctacaataacaagaga
gtcattttgcagtaatttatcatgttcaactgagaacgaccattcaaaaataactgcattaagaatccaagttacacctgtggattacctacttggggta
gctgaccttactggagaattaatgcgttattgtataagtagtgttggaaacggagacattgacacaccctttgagctaagttgtttcctgcgccaggtct
ttgatggattcgcatacattgg
3' EST: BJ638470 [+]tatgaaatgtgcagcacaggtgtaaaatgtacttatttatataaaatacaagcttatgctattatacagccctcttgcaaagaatgatttttactgcaag
agaatccatttaaattctgtcatcaatttcaattaactctgatttgaaagaaaacacatcagcaagcatatgttttggaatctctgaccctctcactttt
aatgcataacaagcattctcaacttttgacagactctgttttaaaacatggatctttcttgaaatttcatatggcccagtgttgccaatgtatgcgaatc
catcaaagacctggcgcaggaaacaacttagctcaaagggtgtgtcaatgtctccgtttccaacactacttatacaataacgcattaattctccagtaag
gtcagctaccccaagtaggtaatccacaggtgtaacttggattcttaatgcagttatttttgaatggtcgttctcagttgaacatgataaattactgcaa
aatgactctcttgttattgtaggcatgttttccaagccttcaaatatcaactgcttgttaatttcattgatgggtaacaagtgttctggactcaatgaaa
tgtttgaaagtgatagcttcaacatactcttgaagacctggtgtaaatgcccggtgatactgatacatatcttctcccaccaactcctctgcaatttctc
ttattttttgacgaacggtaagtaatttagtctctgcttcagacaatacatcttctttattatgatcactcataattctgtg
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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