XB-CLONE-1862251
Clone Name: XL202j12
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Gene:
yeats2.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ622863 [+]
ttgttgcacagggtgtggctaaagctgttgtgagtagtggaagtggaatgtagtagcccagtcctcacagagtgtgccaaaggcgcaagttacatctgct
gtggctcagaaggctggatctcaaggatcagtgatggcgactctgcagcttcctgcttctaatattccaaatctggccaacctacctcccggcaccaaac
tttacctcacaacgaatagcaagaatccatcaggaaaaggaaaactgctgctcattccccagggagccattctaagggctgcaaacactgctaatctgca
gtctggagcatcctccagtggtggatccggtactgctagcaccaccagtgggttaccccagcaccttacgtacacctcctatattctcaagcagactccc
cagggaacatttttagttggtcagcaatcccaacaggcatctggaaagcaagctgctgcaaatgtgatcgttcaggggaacgtcgccacgtcagcccaag
ggcaacagaccttgaaattaactactggacagaaaggcaatttagttgcacaaacccatagtgcatcagccacagttgccaaaatgactgatggtacagt
aacatcggtacaatctcagacccagaagcctggtactactttgctaagagtggcgggaggagtcataacagcctccagtacgtccttaccatctgtttct
gctaatgctcctgcac
3' EST: BJ639547 [+]ttgggttttattcactttggcatagcctgcaccttccattagctttgcatgcaggaaaatacaaaaactcatgtacataaatacaggtctgcatgtactt
acacaaatacatagttacagtctctctctcacactgacattatttacaaaagaaaacaaaaatcgcaggttctaagttagttgtaagacatgttgcattt
catctctcagtgagaatgtctgggctctagataactaggtggttactccccgcacgtataaaatacaaggttggctcagtcagtacatttcgtggtgcca
cttctagatcttatttcaaggtttgctcatcgtccgtagttaatgaggccatgtgtttgttagtgaggaagtcgaataaaggagtgttacagatggcgtg
gtggatatttgatactgtgatctctcgtggagctctattgtgggaggtcatctgacagcccgcagcgaaagcatcccgaaggacgtcactaacaaactgt
tcggtagcctttaaaatcatttcttcaatagcagaagtataaatattgtcctgcacttcaactggttgtaatgaaacgccaatctcctgcgctttatctt
tgatgaactcacaagcaggactggtagccatgtacaactttagatcttcctccgcatcgctctctttcttaatgtgcagtgtcggttcttcactcgtact
cagcacatccacctcctcatccatttccatttccatttcctttttatacgatttctctagttcctccatttttttcaaa
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: