XB-CLONE-1857766
Clone Name: XL211e08
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Gene:
zscan29.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ625339 [+]
tcggcacgagggttagagaggaaagggcttcatgggaagaagagaagcaacttgcagaacaaacacaaggaacagaaacatcgactctcatggggcagag
caagaacagtagcttgttctgcaagataaatacagttacaggacagatattgcagacagataaacaagatgattatatatcatctgttattaaggaggag
tcagcttcatgtgaatgggggaaccccattgaatcagaatgcaatgagcgccataaacaatgtattcataatagagactttgataaccatgaaaaaaaac
acaaaagaaagaaaactttttcttgtttggaatgtggaaaatgttttctaatccaatcacgccttaaagctcatcacaaaatccacatgggagaaaaacg
tttttctcgtttagaatatgggaaaagtttttcaaacaaatcactccttagaaatcatcgcagaacccacacaggagagaaaccattttcttgttctgaa
tgtgagaaaagtttttatactttatcacaacttactgtccatcaacgacaaacccacacaggaaagagaaatttttcttgttcagaatgtggaagatgtt
tttcaaacaaaacacgccttagagagcatcacagaacccacacaggagagaaaccattttcttgttctgaatgcgggaaatgtttttttacttcatcaga
acttactctccatcgacgacagatccccacagggggagaaacctttttcttgnttggaatgnggaaaatggttttcagttcgatcacgccttaaagctca
tcagaaaatccacccaggggagaaacctttttcttggtcanaatg
3' EST: BJ641789 [+]ttgatttttagattaaaaaaagtcacatacattttgatataaaacctttgataaatctgctcattaagactatggttaagtgcaaaacatttcccacatt
cagaacatgaacatgggttctcccctgtgtggatttattgcatgtaaacagatcagatgatgtggcaaaacaattcagacatgcatattaagatataatt
gatgtttgacatcacacaatgacaataagctttatggttttcctcttgtgtgaatctgaaagtgcatttttagactactgctatgtttaaaacatttccc
acatacagaacaagaaaaaggtttctctcccatgtgggttctgtgatgatttttaaggcctgcttgagatgaaaaacacttcccacattctgaacaagaa
aaaggtttctctcctatgtgggttcgtcgttgatgaacagtaagttgtgatgaagtggcaaaacatttcccacattctgaacaagaaaaaggtttctctc
ctgtgtggattcgtcgtcgatgaacctgaagtcctgatgaagtgttaaaacatttcccacactcagaacaagaaaaaggtttctctcctgtgtgagttct
gtggtggtttgtaaagtctgctttatatgaaaaacacttcccacattctgaacaagaaaaangtttctctcctatgtgggttcgtcgttgataaacagta
agttgtgatgaagtggcaaacatttcccacattctgaacaanaaaaangnttctctcctgggnggattcnccgncgatnaacctgaagtcctgatgaagg
gtaaaacnttttcccccctcaaaaaaaaaaaaggtcccctccgggggantccnggggggntttgaaaagcngcnttanaanaaaananttncc
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: