XB-CLONE-1857036
Clone Name: XL212i12
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Gene:
smad4.L
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Species: Xenopus laevis
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Sequence:
5' EST: BJ625746 [+]
gaggtggaagtgtagtagtggggccgactggtggttatttgcttgccgcctgaggagagggaggggacgggctcgagcagggtttatacacaatccctag
tgaattaatttgcctgttatacgtttttctcagcttcccgctccgtagagtatgatgggggttgtagttcagcagcccgtattatataggaaccaataat
acagtcatttccatgtacacgttattactgctccagaaacccgtgagaccaacaagaagccacaatccctagtcatgtgccaactccccgcagcctctag
acatgcgggcttcattgataaggcagttgattgcaagctcccaggccaagtctggtactgcctcatacggtgataataataataataatacatacatgag
gattcattgatcccttattgctctaatccagtctcacgtggagtcattctgttgcttttagtggtcaaaatgctgctgctgctctaaatactatttaatg
gctagtagcctggctcacacacatatggggcacacaatttaatggctagtagcctggctcacgcacatatgggacacactatttaatggctagtagcctg
gctcacacacatatgggacncactatttaatggctagtagcctggctcacacacatatggggcacaccaatttaatggctagtagccctggnctcacgca
catatgggggcacacnaanttaaatgggctagtanccctggntcccncctatagggacacnctattaaatgggctaggtagcctgggntcacacatatnn
ggacacccctatttaa
3' EST: BJ642154 [+]ttatggacaggccagtaatgtccaggatggggcatgggtgggtgatgctgaagatgtccgttcggatggtgagacatattaggtgtgtaagctgctgttc
tacttccagtccaggttgtagtactgttatgatgatatgttgctggctgagcactgaagccattttgtgtcccttggggaggcacaggagcgatggagag
cagcccatcgttgtgagttgctgccagtaaacttgctggttgacttgttgatgggacaggaatgcttgagaaactggtgggattgggtgtgctgggttca
ctcggtaccaacagggctgggttgctataagtttcagtggaggacctgctgcttggtgggtgctggatggtctgaattgaaagtccatctcctgcagaaa
ttgactgctgcccttccgcatagtcgtggccatattcatccttcaccaacaagccggatggggcagaactctgcagcgtcagtcctgaaagatctattcc
aggggatacaactcgttcgtagtggtaaggattgacacaaacactgtcacattttagatcaaaagcatactgacagtacttcacatgcttcagttcattc
ttgtgaaggtctggccacctccaaaggcgagcatagatgacatgggggaagccttttctaccggcaacctgcagccggccatctaacgtcctctgaat
Library:
Description: Briefly, cDNA libraries were constructed with ZAP-cDNA Gigapack III cloning kit (Stratagene) according to manufacturer's instruction with minor modification. 5 ug of poly A+ RNA from each of the three embryonic stages was oligo dT primed (XhoI dT primer). After second strand synthesis, EcoRI adapters were ligated and the cDNA was directionally cloned with EcoRI at the 5' end and XhoI at the 3' end. Normalization was carried out with ssDNA phagemids hybridized to biotinylated driver, which was prepared from the same library by PCR. The average insert size of about 1.5kb. The vector used is pBlueScript SK(-) (Stratagene) and the host bacteria is DH10B (GIBCO).Vector Details | Vector Map |
Name: pBluescript SK-
Type: phagemid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: