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XB-CLONE-1844278
Clone Name: IMAGp998O198088 | Gene: ubac1.L | Species: Xenopus laevis | |
Sequence:
5' EST: BX845136 [+]
Library:
Name: Soares NXEG | External Dbs: Unigene laevis cDNA library, Xenopus IMAGE cDNA Library |
Description: 1st strand was primed with a Not I - oligo(dT) primer[5' TGTTACCAATCTGAAGTGGGAGCGGCCGCGAATCTTTTTTTTTTTTTTTTTTTT 3']; double-stranded cDNA was ligated to EcoRI adaptors 5'-AATTCGGCACGAGG-3' and 5'-CCTCGTGCCG-3' (Pharmacia), digested with NotI and cloned into the NotI and EcoRI sites of the pT7T3D-PacI vector. Library went through one round of normalization, and was constructed in the laboratory of M. Bento Soares (University of Iowa).
Anatomy: egg | Stage: unfertilized egg stage |
Normalized: No | Subtraction: No |
Vector Details | Vector Map | Name: pT7T3D-PacI Type: plasmid 5' Restriction Site: EcoRI 3' Restriction Site: NotI |
Usage:
Suppliers:
Search for clone at: Open Biosystems