XB-CLONE-1820234
Clone Name: cfd-761
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Gene:
cfd.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 761. Complement factor D (adipsin).
To Generate Probe for In Situ.
Clone previously called: IMAGE:7204180
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Sequence:
5' EST: CK797755 [+]
acatgaatatctccagggttctgtttgcggtggtcctggtactaactgtttccacctatgaatgcaggccacgtgggcgtattctaggtgggcaagattc
aaaggaaaaaacctggccttttatggcatctattcagaagaatgaggtccaccaatgtgggggggtccttatttctgataaatgggtgctgagtgcagca
cactgtgctactgacagcaataattcatcactgcatgtgatgcttggcgccatctctctcaccaaaccagagcaatacaaaatagttttaaaggtggaaa
gggagatccctcaccctctgtacaattctaccaaaaagcatcatgatcttctcctgctagagctatctgaaaaagtaacattaagcgaagctgtgaagcc
cctgccttttgaaactgaaaatattgacatccctgatggaaaacgatgcctagtcgcaggttggggacagatgaaatcaactggaaagaagccagacact
ttgcaagaattatggatccccgtgattagcagggatgtctgtaatcgccggaactactatgataatgaaattaccccaaatatgatatgtgctatagaag
ccaagaaggactcatgtgagggagactccgggggacctctagtatgtgatggaatagcagtggctattgtacagggcggatatcgtagatgtggcctatc
gaagaagccaggcatctacacactcattgcaccttacaaatcctggataatggaaaccatgtatgatgctactctactcccatcccctttgtgaaaccat
agtggtaagcctgtagcaacacgataaatcttctaataaatgaggcactgcgttcagtcagtttaattctgttaaagaagat
Library:
Description: RNA obtained from 6 adult male testes. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Expressed on Endothelial cells and blood vessels.
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
EcoRI |
EcoRV and SmaI can also be used. |
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Suppliers:
Search for clone at:
EXRC