XB-CLONE-1818366
Clone Name: tbx1-860
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Gene:
tbx1.L
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Species: Xenopus laevis
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Description: EXRC Clone Number 860.
Xenopus laevis T-box 1.
Image EST 7206683, Accession BC108602.
Full length.
To generate probe for in situ.
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Sequence:
Full length sequence: BC108602 [+]
AGGGAACTGAGCGCACCTTCCCCATTCTGCTGCCCCACAGCCACTGCTCGGGATCCCCGCACCTTCCCAACCCCTACCCCACGGACAGAGTGGAGCAGCA
GCAGCACATTAACCCTTTGTTTTCTCCCCCCTCTTCCCTGTATATTCCTGGGATGATCTCGGCCATCTCCAGCCCCTGGCTCACCCAGCTCTCCCATTTT
TGCGATGTTGCAGCTTTCACGGCGAACAGCCTGAGCAGCTTGAACGCTTCGGGGGGCTACCACCTCTCCCCTTCCCCGGGGGACCCGTACAGCCAGCACG
AACCCCACTACGAACCCTGCTCCGCCAGCCAACACTCGTACAGCTTCGGCCATGCCTGCCCAGAGCCTGAGAGCGGAGCCTCGTCCTCTAGTTGCGCCTC
CTCCACCCCGGGGAGCGGCAGTACAGGCAGCAGCAGCGGCAACAAGGCTCCGGTGAAGAAGAACCCCAAGGTGGCCAACATCACCGTGCAGCTGGAGATG
AAAGCTCTGTGGGACGAGTTCAACCAGCTGGGCACTGAAATGATAGTCACCAAGGCTGGCAGGCGTATGTTCCCCACCTTCCAGGTTAAGATTTTTGGGA
TGGATCCCATGGCCGATTACATGTTATTAATGGACTTTGTACCAGTGGACGATAAACGGTATCGGTATGCCTTTCACAGCTCCTCGTGGTTAGTGGCAGG
CAAAGCAGACCCGGCCACACCCGGTAGAGTTCACTATCACCCGGATTCTCCAGCCAAGGGGGCTCAGTGGATGAAGCAGATCGTGTCCTTTGACAAACTG
AAACTGACAAATAACTTATTGGACGACAACGGGCACATCATCCTGAACTCCATGCACAGGTACCAGCCCCGCTTCCATGTGGTCTACGTGGATCCGAGGA
AGGACAGCGAGAAGTACGCAGAGGAGAACTTTAAAACGTTCGTGTTCGAAGAGACGCGCTTTACGGCAGTGACGGCCTATCAGAACCACCGGATTACACA
ACTGAAAATTGCTAGCAATCCATTTGCCAAGGGATTCAGAGACTGTGACCCAGAAGACTGGCCCAGAAATCATAGACCTGGATCCCTCCCTCTTATGAAT
GCTTTTGCAAGGTCTAGGAATCCTGTCTCCTCCCCTCCACAGAATGGAAGTGATAAAGATGGAGATGGCCGAAGAGAATATGAAAGAGATGCTAGTGTTA
CACCTCTGCATGGGGATGCAGCACATCAACAGCTTATGTCACGAGTACTCAGCCCGTCCCTGCCTGTTCCTGGTGGACTTGTACCTCTCAGTACTGGCAG
ACCCAGCCCCCCACATGAACTGCGCCTAGACCCCCATTCTCAAGGCTCAGAACCACTGCACCACCATCCTTACAAGTACCCAACCAGCTACGATCATTAC
CTAGGAGCAAAAACCCGACCGGCCCCCTACCCTTTGCCAAGTATCAGAGGACATGGCTACCATCATCACCCTATGAACCCAGCAGCAGCAAATATGTACT
CGGGAGCTGGTGCACCAGGCGGTTATGAATACGGGCCAAGATAACATCTGCAGCAGGAGCCTATACTTATTGAATGACTATTTAATAAATATATAAGACA
ATGGATGTTTCATGCATGATTTTAATTATTCAGTAGTGTATTGGATATTACATTACTGTTAAAATGAAAGCTGACCTCTCCCTAAAGTCACATGGGCAGC
CCTGCAGCTTTATCAGTAATTTGTGAAAATCATCATGCAAACAAACTGGATCATCTAACAAATCCTGCTCGAGCTGTTCCGATATTACATCAGCAGAAAG
CAAGCAAAGGCACTTGAGTTTGTCGTCTTTTTTCTTTTATTTGTTCAAATGCTAACTGGAAACAGAGGACAGACACGTTGAAGAGGAGGTGTCCAGGGAC
CTCTGTACATGCACATGTTTATTAAAGCTCCAAGTCTCCTTCTGCTTGTGGAGGAGATAGTTCATCCCACCCGACCTCCACAGATGCACTGTTCAATTAC
TAACTGACATCAGTCCACCCACCAAGGGCATTGACTTTTATGGGGCCTGGTGCACTAATAACGCTTGTAAGAAGCCATATACTTTGTTTTGCTGTTGGTT
TTATTTTCAATTTCTTGAAAAAAAATGTTCAGTTGGCTCAGGTGCAGAGGAATTCTGAGGCGATATGTAGTAAGCTACTTCTACCTGCCACTGTTGTTAC
CATAATCCACCCACTCATAAAAAATTTGTTCTTCCAAAGCCATTTATCCATAACCCCTACTGACTGCATTAGTATGTAATGTCTCATCAAGAAATGTGTG
TTTTGTGACTCAAACAATTATAAATTTGCAGTGTATTTTCAAAATGTAAGTCTGTTTACCTCCCCTAGATTTTTTTTTATCCAGTGACAAACTATAGGAA
GGTGGGTGTACGGAAGTTTCCCATGATATTGCAGAACTATCATTTATCAAAGGAATCCATCCAGGTTGAAATATGGATCTCTAAGGGCAGGAGTTTCTAA
AGTTACGTTTTTCTTTCTTCTTATTTAACATGAAATTTTCATTTTGCACTTCATTAACACGCAGTACACAGTGTACTGGAGCAAAATCATGGCGAATATG
CTGTTTCTGGGGGGGGGGGGGCAAAGGGTGTAAACTGAAAAAATAAAACAGTAAACCAATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAA
5' EST: CK798689 [+]ggaactgagcgcaccttccccattctgctgccccacagccactgctcgggatccccgcaccttcccaacccctaccccacggacagagtggagcagcagc
agcacattaaccctttgttttctcccccctcttccctgtatattcctgggatgatctcggccatctccagcccctggctcacccagctctcccatttttg
cgatgttgcagctttcacggcgaacagcctgagcagcttgaacgcttcggggggctaccacctctccccttccccgggggacccgtacagccagcacgaa
ccccactacgaaccctgctccgccagccaacactcgtacagcttcggccatgcctgcccagagcctgagagcggagcctcgtcctctagttgcgcctcct
ccaccccggggagcggcagtacaggcagcagcagcggcaacaaggctccggtgaagaagaaccccaaggtggccaacatcaccgtgcagctggagatgaa
agctctgtgggacgagttcaaccagctgggcactgaaatgatagtcaccaaggctggcaggcgtatgttccccaccttccaggttaagatttttgggatg
gatcccatggccgattacatgttattaatggactttgtaccagtggacgataaacggtatcggtatgcctttcacagctcctcgtggttagtggcaggca
aagcagacccggccacacccggtagagttcactatcacgcggattctccagccaggggggctcagtggatgaagcagatcgtgtcctttgacaaactgaa
actgacaaataaattattggaacaaaacgggccccttcatcctgaaatccctggccacgggatccgcccccgctttcctgttgggtctccggtggaattc
caagaaagg
Library:
Description: RNA obtained from 6 adult male testes. cDNA was primed using oligo-dT primer: 5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)25-3' and cloned into the EcoRV/NotI sites of pExpress-1. Size-selection >1kb resulted in an average insert size of 1.15 kb. This primary, microquantity library is normalized to Cot5 (non-normalized primary library is NICHD_XGC_Te2) and was constructed by Express Genomics (Frederick, MD). Note: this is a Xenopus Gene Collection library.Anatomy: testis | Stage: |
Normalized: No | Subtraction: No |
Vector Details | Vector Map |
Name: pExpress-1
Type: plasmid
5' Restriction Site: EcoRV
3' Restriction Site: NotI | |
Usage:
Whole mount in situ hybridization:
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Expressed in branchial arches.
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Probes:
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Type |
Size |
Promoter |
Linearization Site |
Description |
antisense |
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T7 |
SacII |
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Suppliers:
Search for clone at:
EXRC